################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MroI MseI MslI MspA1I MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AciI 235 GCGG CCGC(-3/-1) AfaI 156 GTAC GT^AC AspHI 130 GAGCTC G[AT]GC[AT]^C BalI 59 TGGCCA TGG^CCA BanI 280 GGTGCC G^G[CT][GA]CC BanII 130 GAGCTC G[GA]GC[CT]^C BseRI 127 GAGGAG GAGGAG(10/8) BsiHKAI 130 GAGCTC G[AT]GC[AT]^C BsrDI 184 GCAATG GCAATG(2/0) BsrI 111 ACTGG ACTGG(1/-1) BstAPI 219 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 56 GGATG GGATG(2/0) Cac8I 215 GCCTGC GC[ACGT]^[ACGT]GC EaeI 59 TGGCCA [CT]^GGCC[GA] FokI 56 GGATG GGATG(9/13) HapII 54 CCGG C^CGG HincII 163 GTCAAC GT[CT]^[GA]AC HindII 163 GTCAAC GT[CT]^[GA]AC HpaII 54 CCGG C^CGG HphI 35 TCACC GGTGA(8/7) MboII 169 GAAGA GAAGA(8/7) MluNI 59 TGGCCA TGG^CCA MscI 59 TGGCCA TGG^CCA MspI 54 CCGG C^CGG NlaIII 90 CATG CATG^ RsaI 156 GTAC GT^AC SacI 130 GAGCTC GAGCT^C SmlI 196 CTCAAG C^T[CT][GA]AG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaII 119 GGACC G^G[AT]CC 143 GGACC BmyI 14 GTGCCC G[AGT]GC[ACT]^C 130 GAGCTC BsaJI 146 CCTTGG C^C[ACGT][ACGT]GG 176 CCAAGG BsiYI 18 CCTCCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 21 CCTGCTGGTGG BslI 18 CCTCCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 21 CCTGCTGGTGG BsmFI 118 GGGAC GGGAC(10/14) 142 GGGAC Bsp1286I 14 GTGCCC G[AGT]GC[ACT]^C 130 GAGCTC DraII 118 GGGACCC [GA]G^G[ACGT]CC[CT] 142 GGGACCT EcoO109I 118 GGGACCC [GA]G^G[ACGT]CC[CT] 142 GGGACCT EcoT14I 146 CCTTGG C^C[AT][AT]GG 176 CCAAGG PpuMI 118 GGGACCC [GA]G^G[AT]CC[CT] 142 GGGACCT PstI 204 CTGCAG CTGCA^G 217 CTGCAG SfaNI 172 GATGC GCATC(5/9) 47 GCATC SfcI 204 CTGCAG C^T[GA][CT]AG 217 CTGCAG StyI 146 CCTTGG C^C[AT][AT]GG 176 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AluI BbvI Cfr13I Fnu4HI HaeIII ItaI MnlI MwoI NlaIV Sau96I TseI