Restriction map for
pFN21AE0041
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRV
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tsp45I XbaI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 98 CCGC CCGC(-3/-1)
AlwNI 130 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 429 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 263 CTCGAG C^[CT]CG[GA]G
BanI 152 GGCACC G^G[CT][GA]CC
BanII 668 GAGCCC G[GA]GC[CT]^C
BcnI 495 CCCGG CC^[GC]GG
BsaAI 883 TACGTG [CT]AC^GT[GA]
BsaBI 513 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1222 CTCCTC GAGGAG(10/8)
BsoBI 263 CTCGAG C^[CT]CG[GA]G
BspHI 1265 TCATGA T^CATGA
BspMI 21 GCAGGT ACCTGC(4/8)
BsrBI 98 CCGCTC CCGCTC(-3/-3)
BstBI 695 TTCGAA TT^CGAA
Cac8I 615 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 1402 GCGC GCG^C
DraI 685 TTTAAA TTT^AAA
EcoRI 1033 GAATTC G^AATTC
EcoT14I 1394 CCTTGG C^C[AT][AT]GG
EcoT22I 471 ATGCAT ATGCA^T
HapII 496 CCGG C^CGG
HhaI 1402 GCGC GCG^C
HinP1I 1402 GCGC G^CGC
HpaII 496 CCGG C^CGG
MaeIII 1387 GTTAC ^GT[ACGT]AC
MamI 513 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 332 CAGCTG C[AC]G^C[GT]G
MspI 496 CCGG C^CGG
NciI 495 CCCGG CC^[GC]GG
NsiI 471 ATGCAT ATGCA^T
NspV 695 TTCGAA TT^CGAA
PacI 175 TTAATTAA TTAAT^TAA
PaeR7I 263 CTCGAG C^TCGAG
PflFI 429 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 930 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 283 GAGTC GAGTC(4/5)
PshAI 206 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 332 CAGCTG CAG^CTG
RcaI 1265 TCATGA T^CATGA
SapI 100 GCTCTTC GCTCTTC(1/4)
SexAI 7 ACCTGGT A^CC[AT]GGT
SfaNI 315 GATGC GCATC(5/9)
SfuI 695 TTCGAA TT^CGAA
SspI 1141 AATATT AAT^ATT
StyI 1394 CCTTGG C^C[AT][AT]GG
Tth111I 429 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 930 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1289 CCACACCTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 263 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 570 GTAC GT^AC
588 GTAC
AflIII 1184 ACATGT A^C[GA][CT]GT
1213 ACATGT
BsaJI 239 CCCTGG C^C[ACGT][ACGT]GG
1394 CCTTGG
BsmFI 213 GTCCC GGGAC(10/14)
1452 GTCCC
BspLU11I 1184 ACATGT A^CATGT
1213 ACATGT
Cfr13I 95 GGGCC G^G[ACGT]CC
618 GGCCC
DdeI 548 CTAAG C^T[ACGT]AG
845 CTGAG
EcoNI 609 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
630 CCTGGATGAGG
MaeII 596 ACGT A^CGT
884 ACGT
PstI 27 CTGCAG CTGCA^G
613 CTGCAG
RsaI 570 GTAC GT^AC
588 GTAC
Sau96I 95 GGGCC G^G[ACGT]CC
618 GGCCC
SfcI 27 CTGCAG C^T[GA][CT]AG
613 CTGCAG
SmlI 200 CTCAAG C^T[CT][GA]AG
263 CTCGAG
TfiI 841 GATTC G^A[AT]TC
1087 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI Sau3AI ScrFI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I