(Circular) (Six-base) MAPSORT of: pFN21AE0041.seq Check: 9262 from: 1 to: 6265 pFN21AE0041 6265 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 3002 4328 5712 5980 1632 Size: 602 768 1326 1384 268 1917 Fragments arranged by size: 1917 1384 1326 768 602 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 3189 3218 5164 6141 1203 Size: 1986 29 1946 977 1327 Fragments arranged by size: 1986 1946 1327 977 29 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 3299 3377 1166 Size: 2133 78 4054 Fragments arranged by size: 4054 2133 78 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2052 2084 2052 Size: 32 6233 AlwNI CAG_nnn'CTG Cuts at: 2140 4008 5580 2140 Size: 1868 1572 2825 Fragments arranged by size: 2825 1868 1572 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 2053 2403 2747 3038 3341 3574 3800 3952 Size: 560 350 344 291 303 233 226 152 Cuts at: 3952 6061 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 560 350 344 303 291 233 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2268 3590 1870 Size: 73 325 1322 4545 Fragments arranged by size: 4545 1322 325 73 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2157 3586 5041 618 Size: 325 217 627 370 1429 1455 1842 Fragments arranged by size: 1842 1455 1429 627 370 325 217 BanII G_rGCy'C Cuts at: 1391 2677 3584 1391 Size: 1286 907 4072 Fragments arranged by size: 4072 1286 907 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2710 3192 3457 928 Size: 971 811 482 265 3736 Fragments arranged by size: 3736 971 811 482 265 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BfrBI ATG'CAT Cuts at: 2478 2478 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4876 136 Size: 107 122 71 631 605 3204 1525 Fragments arranged by size: 3204 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 2160 2501 3020 4326 5482 1790 Size: 370 341 519 1306 1156 2573 Fragments arranged by size: 2573 1306 1156 519 370 341 BmgBI CAC'GTC Cuts at: 1318 2602 1318 Size: 1284 4981 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3996 4954 442 Size: 416 53 83 399 24 2579 958 1753 Fragments arranged by size: 2579 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2278 2522 2929 4925 1239 Size: 1039 244 407 1996 2579 Fragments arranged by size: 2579 1996 1039 407 244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2190 4390 5255 5553 2190 Size: 2200 865 298 2902 Fragments arranged by size: 2902 2200 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2890 4056 493 Size: 711 1686 1166 2702 Fragments arranged by size: 2702 1686 1166 711 BsaBI GATnn'nnATC Cuts at: 2522 6259 2522 Size: 3737 2528 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4072 4454 5089 275 Size: 53 83 186 936 2539 382 635 1451 Fragments arranged by size: 2539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2052 2082 1178 Size: 30 165 30 649 30 5361 Fragments arranged by size: 5361 649 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3218 1186 Size: 2032 4233 BseYI C'CCAG_C Cuts at: 2579 2586 3254 5468 5964 2579 Size: 7 668 2214 496 2880 Fragments arranged by size: 2880 2214 668 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5692 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4326 4411 5482 4326 Size: 85 1071 5109 Fragments arranged by size: 5109 1071 85 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2501 2677 3020 3584 4326 4411 Size: 399 370 341 176 343 564 742 85 Cuts at: 4411 5482 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 564 399 370 343 341 176 85 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 3270 4154 5884 3270 Size: 884 1730 3651 Fragments arranged by size: 3651 1730 884 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2017 3620 844 Size: 1173 1603 3489 Fragments arranged by size: 3489 1603 1173 BsrBI CCG'CTC Cuts at: 1394 1823 2105 4152 1394 Size: 429 282 2047 3507 Fragments arranged by size: 3507 2047 429 282 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 4934 65 Size: 1183 3512 174 1396 Fragments arranged by size: 3512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4909 5096 1700 Size: 110 3099 187 2869 Fragments arranged by size: 3099 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6265 BstBI TT'CG_AA Cuts at: 2701 5069 2701 Size: 2368 3897 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstYI r'GATC_y Cuts at: 1972 3595 3962 4347 4364 5816 5902 5914 6260 Size: 1623 367 385 17 1452 86 12 346 Cuts at: 6260 1972 Size: 1977 Fragments arranged by size: 1977 1623 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4774 Fragments arranged by size: 4774 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 3781 4575 4595 3781 Size: 794 20 5451 Fragments arranged by size: 5451 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 2692 3569 2692 Size: 877 5388 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3712 4601 6230 Size: 54 1425 50 275 18 1882 889 1629 Cuts at: 6230 8 Size: 43 Fragments arranged by size: 1882 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2112 2280 2755 4195 1353 Size: 759 168 475 1440 3423 Fragments arranged by size: 3423 1440 759 475 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4852 5226 5372 387 Size: 1064 138 3263 374 146 1280 Fragments arranged by size: 3263 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2278 2522 2929 3002 4328 4925 Size: 393 602 44 244 407 73 1326 597 Cuts at: 4925 5712 5980 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1326 787 602 597 407 393 268 244 73 44 EcoNI CCTnn'n_nnAGG Cuts at: 2618 2639 2618 Size: 21 6244 EcoRI G'AATT_C Cuts at: 3038 3574 3952 3038 Size: 536 378 5351 Fragments arranged by size: 5351 536 378 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 5412 1678 Size: 3734 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2273 2305 2542 2574 3427 Size: 32 135 32 701 32 237 32 853 Cuts at: 3427 3459 4906 4938 4980 5012 1373 Size: 32 1447 32 42 32 2626 Fragments arranged by size: 2626 1447 853 701 237 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3067 3285 5379 5563 1305 Size: 1762 218 2094 184 2007 Fragments arranged by size: 2094 2007 1762 218 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4443 Fragments arranged by size: 4443 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2428 3206 3299 3377 4223 4582 4741 Size: 648 1262 778 93 78 846 359 159 Cuts at: 4741 518 Size: 2042 Fragments arranged by size: 2042 1262 846 778 648 359 159 93 78 MspA1I CmG'CkG Cuts at: 1606 2339 3681 4358 5506 5751 1606 Size: 733 1342 677 1148 245 2120 Fragments arranged by size: 2120 1342 1148 733 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4774 Fragments arranged by size: 4774 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NsiI A_TGCA'T Cuts at: 2480 2480 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2482 3193 3222 3623 5168 1132 Size: 97 1253 711 29 401 1545 2229 Fragments arranged by size: 2229 1545 1253 711 401 97 29 PacI TTA_AT'TAA Cuts at: 2184 2184 Size: 6265 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 3189 3218 5164 3189 Size: 29 1946 4290 Fragments arranged by size: 4290 1946 29 PflMI CCAn_nnn'nTGG Cuts at: 1108 2941 1108 Size: 1833 4432 PfoI T'CCnGG_A Cuts at: 2634 2634 Size: 6265 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2052 2084 4334 4366 5847 5879 2052 Size: 32 2250 32 1481 32 2438 Fragments arranged by size: 2438 2250 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2215 1613 Size: 602 5663 PsiI TTA'TAA Cuts at: 3069 3835 3069 Size: 766 5499 PstI C_TGCA'G Cuts at: 830 2036 2622 3617 830 Size: 1206 586 995 3478 Fragments arranged by size: 3478 1206 995 586 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2339 2339 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SapI GCTCTTCn'nnn_ Cuts at: 2112 2112 Size: 6265 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SexAI A'CCwGG_T Cuts at: 2012 2012 Size: 6265 SfcI C'TryA_G Cuts at: 826 1045 2032 2618 3613 4748 5429 5620 826 Size: 219 987 586 995 1135 681 191 1471 Fragments arranged by size: 1471 1135 995 987 681 586 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2205 2268 4369 5270 5532 820 Size: 197 926 262 63 2101 901 262 1553 Fragments arranged by size: 2101 1553 926 901 262 262 197 63 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 3148 4189 5 Size: 47 3096 1041 2081 Fragments arranged by size: 3096 2081 1041 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6265 StyI C'CwwG_G Cuts at: 513 1065 2004 3399 3704 513 Size: 552 939 1395 305 3074 Fragments arranged by size: 3074 1395 939 552 305 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2600 3570 4292 4309 4462 4647 5092 Size: 1255 1020 970 722 17 153 185 445 Cuts at: 5092 325 Size: 1498 Fragments arranged by size: 1498 1255 1020 970 722 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2592 4511 6223 Size: 274 80 33 51 1443 615 1919 1712 Cuts at: 6223 96 Size: 138 Fragments arranged by size: 1919 1712 1443 615 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6225 6257 6225 Size: 32 6233 Tth111I GACn'n_nGTC Cuts at: 1316 2437 1316 Size: 1121 5144 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3301 3301 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 2268 1943 Size: 325 5940 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI