Restriction map for
pFN21AE0041
(Linear) (Six-base) MAPSORT of: KIEE0041.seq Check: 8299 from: 1 to: 1560
KIEE0041.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 997 1560
Size: 229 768 563
Fragments arranged by size:
768 563 229
AflIII A'CryG_T
Cuts at: 0 1184 1213 1560
Size: 1184 29 347
Fragments arranged by size:
1184 347 29
AleI CACnn'nnGTG
Cuts at: 0 1294 1372 1560
Size: 1294 78 188
Fragments arranged by size:
1294 188 78
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 47 79 1560
Size: 47 32 1481
Fragments arranged by size:
1481 47 32
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1560
Size: 135 1425
ApoI r'AATT_y
Cuts at: 0 48 398 742 1033 1336 1560
Size: 48 350 344 291 303 224
Fragments arranged by size:
350 344 303 291 224 48
AvaI C'yCGr_G
Cuts at: 0 263 1560
Size: 263 1297
BanI G'GyrC_C
Cuts at: 0 152 1560
Size: 152 1408
BanII G_rGCy'C
Cuts at: 0 672 1560
Size: 672 888
BbsI GAAGACnn'nnnn_
Cuts at: 0 705 1187 1452 1560
Size: 705 482 265 108
Fragments arranged by size:
705 482 265 108
BfrBI ATG'CAT
Cuts at: 0 473 1560
Size: 473 1087
Bme1580I G_kGCm'C
Cuts at: 0 155 496 1015 1560
Size: 155 341 519 545
Fragments arranged by size:
545 519 341 155
BmgBI CAC'GTC
Cuts at: 0 597 1560
Size: 597 963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 517 924 1560
Size: 273 244 407 636
Fragments arranged by size:
636 407 273 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 1560
Size: 185 1375
BsaAI yAC'GTr
Cuts at: 0 885 1560
Size: 885 675
BsaBI GATnn'nnATC
Cuts at: 0 517 1560
Size: 517 1043
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 47 77 1560
Size: 47 30 1483
Fragments arranged by size:
1483 47 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1213 1560
Size: 1213 347
BseYI C'CCAG_C
Cuts at: 0 574 581 1249 1560
Size: 574 7 668 311
Fragments arranged by size:
668 574 311 7
Bsp1286I G_dGCh'C
Cuts at: 0 155 496 672 1015 1560
Size: 155 341 176 343 545
Fragments arranged by size:
545 343 341 176 155
BspHI T'CATG_A
Cuts at: 0 1265 1560
Size: 1265 295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 12 1560
Size: 12 1548
BsrBI CCG'CTC
Cuts at: 0 100 1560
Size: 100 1460
BstBI TT'CG_AA
Cuts at: 0 696 1560
Size: 696 864
DraI TTT'AAA
Cuts at: 0 687 1560
Size: 687 873
EarI CTCTTCn'nnn_
Cuts at: 0 107 275 750 1560
Size: 107 168 475 810
Fragments arranged by size:
810 475 168 107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 273 517 924 997 1560
Size: 229 44 244 407 73 563
Fragments arranged by size:
563 407 244 229 73 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 634 1560
Size: 613 21 926
Fragments arranged by size:
926 613 21
EcoRI G'AATT_C
Cuts at: 0 1033 1560
Size: 1033 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 537 569 1422 1454 1560
Size: 268 32 237 32 853 32 106
Fragments arranged by size:
853 268 237 106 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1062 1280 1560
Size: 1062 218 280
Fragments arranged by size:
1062 280 218
MslI CAynn'nnrTG
Cuts at: 0 423 1201 1294 1372 1560
Size: 423 778 93 78 188
Fragments arranged by size:
778 423 188 93 78
MspA1I CmG'CkG
Cuts at: 0 334 1560
Size: 334 1226
NsiI A_TGCA'T
Cuts at: 0 475 1560
Size: 475 1085
NspI r_CATG'y
Cuts at: 0 477 1188 1217 1560
Size: 477 711 29 343
Fragments arranged by size:
711 477 343 29
PacI TTA_AT'TAA
Cuts at: 0 179 1560
Size: 179 1381
PciI A'CATG_T
Cuts at: 0 1184 1213 1560
Size: 1184 29 347
Fragments arranged by size:
1184 347 29
PflMI CCAn_nnn'nTGG
Cuts at: 0 936 1560
Size: 936 624
PfoI T'CCnGG_A
Cuts at: 0 629 1560
Size: 629 931
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 47 79 1560
Size: 47 32 1481
Fragments arranged by size:
1481 47 32
PshAI GACnn'nnGTC
Cuts at: 0 210 1560
Size: 210 1350
PsiI TTA'TAA
Cuts at: 0 1064 1560
Size: 1064 496
PstI C_TGCA'G
Cuts at: 0 31 617 1560
Size: 31 586 943
Fragments arranged by size:
943 586 31
PvuII CAG'CTG
Cuts at: 0 334 1560
Size: 334 1226
SapI GCTCTTCn'nnn_
Cuts at: 0 107 1560
Size: 107 1453
SexAI A'CCwGG_T
Cuts at: 0 7 1560
Size: 7 1553
SfcI C'TryA_G
Cuts at: 0 27 613 1560
Size: 27 586 947
Fragments arranged by size:
947 586 27
SmlI C'TyrA_G
Cuts at: 0 200 263 1560
Size: 200 63 1297
Fragments arranged by size:
1297 200 63
SspI AAT'ATT
Cuts at: 0 1143 1560
Size: 1143 417
StyI C'CwwG_G
Cuts at: 0 1394 1560
Size: 1394 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 1560
Size: 595 965
TatI w'GTAC_w
Cuts at: 0 587 1560
Size: 587 973
Tth111I GACn'n_nGTC
Cuts at: 0 432 1560
Size: 432 1128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1296 1560
Size: 1296 264
XhoI C'TCGA_G
Cuts at: 0 263 1560
Size: 263 1297
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApoI AvaI BanI
BanII BbsI BfrBI Bme1580I BmgBI BpmI BpuEI BsaAI
BsaBI BsaXI BseRI BseYI Bsp1286I BspHI BspMI BsrBI
BstBI DraI EarI Eco57MI EcoNI EcoRI Hin4I MmeI
MslI MspA1I NsiI NspI PacI PciI PflMI PfoI
PpiI PshAI PsiI PstI PvuII SapI SexAI SfcI
SmlI SspI StyI TaqII TatI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI BmrI BmtI BplI Bpu10I BsaI BsaHI
BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PasI PmeI PmlI
PpuMI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI
XbaI XmaI XmnI ZraI