Restriction map for
pFN21AE0409
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BpmI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII HphI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TseI Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 191 GTCTAC GT^[AC][GT]AC
AccII 39 CGCG CG^CG
AcsI 107 GAATTC [GA]^AATT[CT]
AfaI 380 GTAC GT^AC
AlwNI 353 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 230 GGGCCC GGGCC^C
ApoI 107 GAATTC [GA]^AATT[CT]
AspHI 22 GAGCTC G[AT]GC[AT]^C
BanI 573 GGTGCC G^G[CT][GA]CC
BbsI 509 GAAGAC GAAGAC(2/6)
BbvCI 342 CCTCAGC CCTCAGC(-5/-2)
BlpI 72 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 72 GCTGAGC GC^T[ACGT]AGC
BpuAI 509 GAAGAC GAAGAC(2/6)
BseRI 19 GAGGAG GAGGAG(10/8)
BsgI 13 GTGCAG GTGCAG(16/14)
BsiHKAI 22 GAGCTC G[AT]GC[AT]^C
BsmFI 94 GGGAC GGGAC(10/14)
BspHI 300 TCATGA T^CATGA
BsrBI 78 CCGCTC CCGCTC(-3/-3)
BssSI 58 CACGAG CACGAG(-5/-1)
BstUI 39 CGCG CG^CG
CelII 72 GCTGAGC GC^T[ACGT]AGC
DpnII 439 GATC ^GATC
DsaI 401 CCATGG C^C[GA][CT]GG
EaeI 35 TGGCCG [CT]^GGCC[GA]
EcoRI 107 GAATTC G^AATTC
EcoT14I 401 CCATGG C^C[AT][AT]GG
HincII 222 GTTGAC GT[CT]^[GA]AC
HindII 222 GTTGAC GT[CT]^[GA]AC
MboI 439 GATC ^GATC
MboII 509 GAAGA GAAGA(8/7)
MslI 396 CACGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 39 CGCG CG^CG
MwoI 15 GCAGGAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 401 CCATGG C^CATGG
NdeII 439 GATC ^GATC
PflMI 352 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 48 CTGCAG CTGCA^G
RcaI 300 TCATGA T^CATGA
RsaI 380 GTAC GT^AC
SacI 22 GAGCTC GAGCT^C
Sau3AI 439 GATC ^GATC
SexAI 195 ACCTGGT A^CC[AT]GGT
SfaNI 136 GATGC GCATC(5/9)
StyI 401 CCATGG C^C[AT][AT]GG
TaqI 205 TCGA T^CGA
Tsp45I 241 GTGAC ^GT[GC]AC
TthHB8I 205 TCGA T^CGA
Van91I 352 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 348 CCAACCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AvaII 30 GGTCC G^G[AT]CC
95 GGACC
BanII 22 GAGCTC G[GA]GC[CT]^C
230 GGGCCC
BmyI 22 GAGCTC G[AGT]GC[ACT]^C
230 GGGCCC
BsaJI 227 CCAGGG C^C[ACGT][ACGT]GG
401 CCATGG
BsmAI 85 GAGAC GTCTC(1/5)
554 GAGAC
Bsp1286I 22 GAGCTC G[AGT]GC[ACT]^C
230 GGGCCC
BstF5I 135 GGATG GGATG(2/0)
405 GGATG
Cac8I 50 GCAGGC GC[ACGT]^[ACGT]GC
267 GCAAGC
DraII 52 AGGCCCC [GA]G^G[ACGT]CC[CT]
229 AGGGCCC
EcoO109I 52 AGGCCCC [GA]G^G[ACGT]CC[CT]
229 AGGGCCC
Fnu4HI 37 GCCGC GC^[ACGT]GC
77 GCCGC
FokI 135 GGATG GGATG(9/13)
405 GGATG
HinfI 318 GATTC G^A[ACGT]TC
327 GAATC
ItaI 37 GCCGC GC^[ACGT]GC
77 GCCGC
MaeIII 241 GTGAC ^GT[ACGT]AC
258 GTTAC
MseI 209 TTAA T^TAA
496 TTAA
SfcI 48 CTGCAG C^T[GA][CT]AG
547 CTACAG
SmlI 262 CTTGAG C^T[CT][GA]AG
568 CTCAAG
TfiI 318 GATTC G^A[AT]TC
327 GAATC
Tru9I 209 TTAA T^TAA
496 TTAA
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AluI BsiYI BslI BsrI
BssKI BstNI Cfr13I DdeI EcoRII
HaeIII MnlI MvaI NlaIII NlaIV
Sau96I ScrFI Tsp509I TspRI