Restriction map for
pFN21AB8903
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# NO CUT ENZYMES ( 146 enzymes) #
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AccI AccIII AclI AflII AflIII
AgeI AhdI AluI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspHI AspI AviII AvrII
BalI BamHI BanII BbrPI BbsI
BbvCI BclI BfaI BfrI BglI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaI BseAI BsgI BsiHKAI
BsiWI BsmBI BsmFI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI DraI
DraII DraIII DrdI DsaI Eam1105I
Eco47III EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
HphI KspI MaeI MaeIII MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tsp45I
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 55 GACGTC GACGT^C
Acc65I 449 GGTACC G^GTACC
AfaI 450 GTAC GT^AC
AlwNI 454 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 272 CCTTAGG CC^T[ACGT]AGG
Asp700I 383 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 449 GGTACC G^GTACC
AvaI 618 CTCGGG C^[CT]CG[GA]G
BbeI 503 GGCGCC GGCGC^C
BbvI 378 GCAGC GCAGC(8/12)
BcgI 512 GCATCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 356 GTATCC GTATCC(6/5)
BglII 437 AGATCT A^GATCT
BpmI 466 CTGGAG CTGGAG(16/14)
BsaBI 552 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 688 GAGGAG GAGGAG(10/8)
BsmAI 679 GAGAC GTCTC(1/5)
BsoBI 618 CTCGGG C^[CT]CG[GA]G
BsrFI 506 GCCGGC [GA]^CCGG[CT]
Bsu36I 272 CCTTAGG CC^T[ACGT]AGG
Cac8I 506 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 506 GCCGGC [GA]^CCGG[CT]
CpoI 416 CGGACCG CG^G[AT]CCG
Eco81I 272 CCTTAGG CC^T[ACGT]AGG
FauI 672 GCGGG CCCGC(4/6)
HaeII 503 GGCGCC [GA]GCGC^[CT]
KasI 503 GGCGCC G^GCGCC
KpnI 449 GGTACC GGTAC^C
MamI 552 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 506 GCCGGC GCC^GGC
NarI 503 GGCGCC GG^CGCC
NgoMIV 506 GCCGGC G^CCGGC
NlaIII 246 CATG CATG^
NotI 428 GCGGCCGC GC^GGCCGC
NspI 245 ACATGC [GA]CATG^[CT]
PflMI 453 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 153 GACTC GAGTC(4/5)
PstI 258 CTGCAG CTGCA^G
RsaI 450 GTAC GT^AC
RsrII 416 CGGACCG CG^G[AT]CCG
SfcI 258 CTGCAG C^T[GA][CT]AG
SfoI 503 GGCGCC GGC^GCC
SgrAI 505 CGCCGGCG C[GA]^CCGG[CT]G
TfiI 595 GAATC G^A[AT]TC
TseI 378 GCAGC G^C[AT]GC
Van91I 453 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 383 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 55 GACGTC G[GA]^CG[CT]C
503 GGCGCC
AvaII 342 GGACC G^G[AT]CC
417 GGACC
BanI 449 GGTACC G^G[CT][GA]CC
503 GGCGCC
BcnI 44 CCGGG CC^[GC]GG
198 CCGGG
BsaHI 55 GACGTC G[GA]^CG[CT]C
503 GGCGCC
BsaWI 140 ACCGGA [AT]^CCGG[AT]
419 ACCGGA
BsiEI 125 CGGCCG CG[GA][CT]^CG
429 CGGCCG
BsrBI 122 GAGCGG CCGCTC(-3/-3)
413 GAGCGG
EaeI 125 CGGCCG [CT]^GGCC[GA]
429 CGGCCG
EagI 125 CGGCCG C^GGCCG
429 CGGCCG
EarI 676 GAAGAG CTCTTC(1/4)
685 GAAGAG
EclXI 125 CGGCCG C^GGCCG
429 CGGCCG
Eco52I 125 CGGCCG C^GGCCG
429 CGGCCG
EcoRI 3 GAATTC G^AATTC
387 GAATTC
Hin1I 55 GACGTC G[GA]^CG[CT]C
503 GGCGCC
HinfI 153 GACTC G^A[ACGT]TC
595 GAATC
Ksp632I 676 GAAGAG CTCTTC(1/4)
685 GAAGAG
MaeII 56 ACGT A^CGT
68 ACGT
MboII 676 GAAGA GAAGA(8/7)
685 GAAGA
MwoI 124 GCGGCCGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
501 GCGGCGCCGGC
NciI 44 CCGGG CC^[GC]GG
198 CCGGG
SfaNI 375 GATGC GCATC(5/9)
512 GCATC
TspRI 98 CAGTG CA[GC]TG[ACGT][ACGT]^
396 CAGTG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AcsI AlwI ApoI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI BstUI BstYI CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MflI MnlI
MspI MvaI MvnI NdeII NlaIV
Sau3AI Sau96I ScrFI TaqI Tsp509I
TthHB8I XhoII