Restriction map for
pFN21AB8903
(Linear) (Six-base) MAPSORT of: KIBB8903.seq Check: 9406 from: 1 to: 705
KIBB8903.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 0 59 705
Size: 59 646
Acc65I G'GTAC_C
Cuts at: 0 449 705
Size: 449 256
AlwNI CAG_nnn'CTG
Cuts at: 0 459 705
Size: 459 246
ApoI r'AATT_y
Cuts at: 0 3 387 475 705
Size: 3 384 88 230
Fragments arranged by size:
384 230 88 3
AvaI C'yCGr_G
Cuts at: 0 618 705
Size: 618 87
BanI G'GyrC_C
Cuts at: 0 449 503 705
Size: 449 54 202
Fragments arranged by size:
449 202 54
BbeI G_GCGC'C
Cuts at: 0 507 705
Size: 507 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 501 535 705
Size: 501 34 170
Fragments arranged by size:
501 170 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 367 705
Size: 367 338
BglII A'GATC_T
Cuts at: 0 437 705
Size: 437 268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 705
Size: 487 218
Bpu10I CC'TnA_GC
Cuts at: 0 302 705
Size: 302 403
BsaBI GATnn'nnATC
Cuts at: 0 556 705
Size: 556 149
BsaHI Gr'CG_yC
Cuts at: 0 56 504 705
Size: 56 448 201
Fragments arranged by size:
448 201 56
BsaWI w'CCGG_w
Cuts at: 0 140 419 705
Size: 140 279 286
Fragments arranged by size:
286 279 140
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 113 143 705
Size: 113 30 562
Fragments arranged by size:
562 113 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 703 705
Size: 703 2
BsiEI CG_ry'CG
Cuts at: 0 128 432 705
Size: 128 304 273
Fragments arranged by size:
304 273 128
BsrBI CCG'CTC
Cuts at: 0 124 415 705
Size: 124 291 290
Fragments arranged by size:
291 290 124
BsrFI r'CCGG_y
Cuts at: 0 506 705
Size: 506 199
BstYI r'GATC_y
Cuts at: 0 143 217 437 705
Size: 143 74 220 268
Fragments arranged by size:
268 220 143 74
Bsu36I CC'TnA_GG
Cuts at: 0 273 705
Size: 273 432
EaeI y'GGCC_r
Cuts at: 0 125 429 705
Size: 125 304 276
Fragments arranged by size:
304 276 125
EagI C'GGCC_G
Cuts at: 0 125 429 705
Size: 125 304 276
Fragments arranged by size:
304 276 125
EarI CTCTTCn'nnn_
Cuts at: 0 671 680 705
Size: 671 9 25
Fragments arranged by size:
671 25 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 644 671 705
Size: 644 27 34
Fragments arranged by size:
644 34 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 705
Size: 487 218
EcoRI G'AATT_C
Cuts at: 0 3 387 705
Size: 3 384 318
Fragments arranged by size:
384 318 3
HaeII r_GCGC'y
Cuts at: 0 507 705
Size: 507 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 235 267 705
Size: 202 32 1 32 438
Fragments arranged by size:
438 202 32 32 1
KasI G'GCGC_C
Cuts at: 0 503 705
Size: 503 202
KpnI G_GTAC'C
Cuts at: 0 453 705
Size: 453 252
NaeI GCC'GGC
Cuts at: 0 508 705
Size: 508 197
NarI GG'CG_CC
Cuts at: 0 504 705
Size: 504 201
NgoMIV G'CCGG_C
Cuts at: 0 506 705
Size: 506 199
NotI GC'GGCC_GC
Cuts at: 0 429 705
Size: 429 276
NspI r_CATG'y
Cuts at: 0 249 705
Size: 249 456
PflMI CCAn_nnn'nTGG
Cuts at: 0 459 705
Size: 459 246
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 130 162 705
Size: 130 32 543
Fragments arranged by size:
543 130 32
PstI C_TGCA'G
Cuts at: 0 262 705
Size: 262 443
RsrII CG'GwC_CG
Cuts at: 0 417 705
Size: 417 288
SfcI C'TryA_G
Cuts at: 0 258 705
Size: 258 447
SfoI GGC'GCC
Cuts at: 0 505 705
Size: 505 200
SgrAI Cr'CCGG_yG
Cuts at: 0 506 705
Size: 506 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 556 705
Size: 556 149
XmnI GAAnn'nnTTC
Cuts at: 0 387 705
Size: 387 318
ZraI GAC'GTC
Cuts at: 0 57 705
Size: 57 648
Enzymes that do cut:
AatII Acc65I AlwNI ApoI AvaI BanI BbeI BcgI
BciVI BglII BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI
BseRI BsiEI BsrBI BsrFI BstYI Bsu36I EaeI EagI
EarI EciI Eco57MI EcoRI HaeII Hin4I KasI KpnI
NaeI NarI NgoMIV NotI NspI PflMI PsrI PstI
RsrII SfcI SfoI SgrAI TaqII XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbsI BbvCI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BseYI BsgI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI