(Circular) (Six-base) MAPSORT of: pFN21AB6415.seq Check: 8920 from: 1 to: 5629 pFN21AB6415 5629 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3439 278 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2430 2972 1937 Size: 493 542 4594 Fragments arranged by size: 4594 542 493 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2379 3692 5076 5344 1632 Size: 747 1313 1384 268 1917 Fragments arranged by size: 1917 1384 1313 747 268 AfeI AGC'GCT Cuts at: 2843 2843 Size: 5629 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 2202 4358 2202 Size: 2156 3473 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5629 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2541 2573 2541 Size: 32 5597 AlwNI CAG_nnn'CTG Cuts at: 2731 3372 4944 2731 Size: 641 1572 3416 Fragments arranged by size: 3416 1572 641 ApaI G_GGCC'C Cuts at: 2096 2096 Size: 5629 ApaLI G'TGCA_C Cuts at: 2571 3686 4842 2571 Size: 1115 1156 3358 Fragments arranged by size: 3358 1156 1115 ApoI r'AATT_y Cuts at: 1493 2938 3164 3316 5425 1493 Size: 1445 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 226 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2161 2222 2954 1870 Size: 73 218 61 732 4545 Fragments arranged by size: 4545 732 218 73 61 BamHI G'GATC_C Cuts at: 2959 3326 2959 Size: 367 5262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2165 2299 2804 2950 4405 Size: 325 217 627 378 134 505 146 1455 Cuts at: 4405 618 Size: 1842 Fragments arranged by size: 1842 1455 627 505 378 325 217 146 134 BanII G_rGCy'C Cuts at: 1391 2096 2948 1391 Size: 705 852 4072 Fragments arranged by size: 4072 852 705 BbeI G_GCGC'C Cuts at: 2303 2303 Size: 5629 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4658 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3820 3854 1903 Size: 34 1883 34 3678 Fragments arranged by size: 3678 1883 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3521 4731 3521 Size: 1210 4419 BclI T'GATC_A Cuts at: 1548 1548 Size: 5629 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2076 2174 4240 Size: 107 122 71 631 605 404 98 2066 Cuts at: 4240 136 Size: 1525 Fragments arranged by size: 2066 1525 631 605 404 122 107 98 71 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 2243 3046 2243 Size: 803 4826 Bme1580I G_kGCm'C Cuts at: 1790 2096 2170 2379 2575 2595 3690 4846 1790 Size: 306 74 209 196 20 1095 1156 2573 Fragments arranged by size: 2573 1156 1095 306 209 196 74 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3360 4318 442 Size: 416 53 83 399 24 1943 958 1753 Fragments arranged by size: 1943 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2560 2560 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4289 1239 Size: 3050 2579 Bpu10I CC'TnA_GC Cuts at: 2248 2248 Size: 5629 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3754 4619 4917 3754 Size: 865 298 4466 Fragments arranged by size: 4466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2647 4292 882 Size: 617 1148 1645 2219 Fragments arranged by size: 2219 1645 1148 617 BsaAI yAC'GTr Cuts at: 493 1204 3420 493 Size: 711 2216 2702 Fragments arranged by size: 2702 2216 711 BsaBI GATnn'nnATC Cuts at: 5623 5623 Size: 5629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2300 3436 3818 4453 Size: 53 83 186 936 767 1136 382 635 Cuts at: 4453 275 Size: 1451 Fragments arranged by size: 1451 1136 936 767 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4062 4460 4734 4881 5292 4062 Size: 398 274 147 411 4399 Fragments arranged by size: 4399 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2032 2062 1178 Size: 30 165 30 629 30 4745 Fragments arranged by size: 4745 629 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5629 BseYI C'CCAG_C Cuts at: 2293 4832 5328 2293 Size: 2539 496 2594 Fragments arranged by size: 2594 2539 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2093 1163 Size: 573 357 4699 Fragments arranged by size: 4699 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2181 2187 3079 3840 4868 1540 Size: 275 186 180 6 892 761 1028 2301 Fragments arranged by size: 2301 1028 892 761 275 186 180 6 BsiHKAI G_wGCw'C Cuts at: 2575 3690 3775 4846 2575 Size: 1115 85 1071 3358 Fragments arranged by size: 3358 1115 1071 85 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2170 2379 2575 2595 2948 3690 Size: 399 306 74 209 196 20 353 742 Cuts at: 3690 3775 4846 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 353 306 209 196 85 74 20 BspEI T'CCGG_A Cuts at: 5292 5292 Size: 5629 BspHI T'CATG_A Cuts at: 3518 5248 3518 Size: 1730 3899 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2151 2984 844 Size: 1307 833 3489 Fragments arranged by size: 3489 1307 833 BsrBI CCG'CTC Cuts at: 1394 1823 2033 3516 1394 Size: 429 210 1483 3507 Fragments arranged by size: 3507 1483 429 210 BsrDI GCAATG_nn' Cuts at: 65 1248 2708 4124 4298 65 Size: 1183 1460 1416 174 1396 Fragments arranged by size: 1460 1416 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2125 4273 4460 1700 Size: 110 315 2148 187 2869 Fragments arranged by size: 2869 2148 315 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5629 BssHII G'CGCG_C Cuts at: 1925 2327 1925 Size: 402 5227 BssSI C'ACGA_G Cuts at: 2603 2634 3689 4701 2603 Size: 31 1055 1012 3531 Fragments arranged by size: 3531 1055 1012 31 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5629 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstXI CCAn_nnnn'nTGG Cuts at: 2401 2401 Size: 5629 BstYI r'GATC_y Cuts at: 1972 2959 3326 3711 3728 5180 5266 5278 5624 Size: 987 367 385 17 1452 86 12 346 Cuts at: 5624 1972 Size: 1977 Fragments arranged by size: 1977 1452 987 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2044 2181 2484 513 Size: 552 979 137 303 3658 Fragments arranged by size: 3658 979 552 303 137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4890 Fragments arranged by size: 4890 683 56 BtsI GCAGTG_nn' Cuts at: 2473 3145 3939 3959 2473 Size: 672 794 20 4143 Fragments arranged by size: 4143 794 672 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5594 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2184 2470 Size: 54 1425 50 275 18 348 6 286 Cuts at: 2470 2481 3076 3965 5594 8 Size: 11 595 889 1629 43 Fragments arranged by size: 1629 1425 889 595 348 286 275 54 50 43 18 11 6 EagI C'GGCC_G Cuts at: 1537 1812 2178 2184 3076 1537 Size: 275 366 6 892 4090 Fragments arranged by size: 4090 892 366 275 6 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 3559 1353 Size: 1053 1153 3423 Fragments arranged by size: 3423 1153 1053 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4216 4590 4736 387 Size: 1064 138 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 3692 4289 5076 5344 1239 Size: 393 747 1313 597 787 268 1524 Fragments arranged by size: 1524 1313 787 747 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2092 2093 2196 2204 2092 Size: 1 103 8 5517 Fragments arranged by size: 5517 103 8 1 EcoRI G'AATT_C Cuts at: 2938 3316 2938 Size: 378 5251 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2548 2863 2902 2548 Size: 315 39 5275 Fragments arranged by size: 5275 315 39 FspI TGC'GCA Cuts at: 2765 4135 2765 Size: 1370 4259 HaeII r_GCGC'y Cuts at: 1678 2071 2156 2303 2322 2732 2845 4776 1678 Size: 393 85 147 19 410 113 1931 2531 Fragments arranged by size: 2531 1931 410 393 147 113 85 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2188 2220 4270 4302 4344 Size: 32 135 32 616 32 2050 32 42 Cuts at: 4344 4376 1373 Size: 32 2626 Fragments arranged by size: 2626 2050 616 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2973 3219 669 Size: 988 281 1035 246 3079 Fragments arranged by size: 3079 1035 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KasI G'GCGC_C Cuts at: 2299 2299 Size: 5629 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4743 4927 1305 Size: 3438 184 2007 Fragments arranged by size: 3438 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2483 10 Size: 54 1425 343 651 3156 Fragments arranged by size: 3156 1425 651 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 2382 2399 2483 2603 2718 3587 Size: 648 960 256 17 84 120 115 869 Cuts at: 3587 3946 4105 518 Size: 359 159 2042 Fragments arranged by size: 2042 960 869 648 359 256 159 120 115 84 17 MspA1I CmG'CkG Cuts at: 1606 2028 2046 2107 2183 2460 2563 2705 3045 Size: 422 18 61 76 277 103 142 340 Cuts at: 3045 3722 4870 5115 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 422 340 277 245 142 103 76 61 18 NarI GG'CG_CC Cuts at: 2300 2300 Size: 5629 NcoI C'CATG_G Cuts at: 513 1065 2484 513 Size: 552 1419 3658 Fragments arranged by size: 3658 1419 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5629 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 2178 3076 2178 Size: 898 4731 NspI r_CATG'y Cuts at: 1132 1229 2125 2987 4532 1132 Size: 97 896 862 1545 2229 Fragments arranged by size: 2229 1545 896 862 97 PasI CC'CwG_GG Cuts at: 1348 2088 2200 1348 Size: 740 112 4777 Fragments arranged by size: 4777 740 112 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 2479 1108 Size: 1371 4258 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2196 2204 2196 Size: 8 5621 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5629 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PspOMI G'GGCC_C Cuts at: 2092 2092 Size: 5629 PstI C_TGCA'G Cuts at: 830 2148 2981 830 Size: 1318 833 3478 Fragments arranged by size: 3478 1318 833 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 PvuII CAG'CTG Cuts at: 2563 2563 Size: 5629 SacII CC_GC'GG Cuts at: 2047 2184 2047 Size: 137 5492 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SanDI GG'GwC_CC Cuts at: 2196 2204 2196 Size: 8 5621 SbfI CC_TGCA'GG Cuts at: 2148 2981 2148 Size: 833 4796 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SexAI A'CCwGG_T Cuts at: 2622 2622 Size: 5629 SfcI C'TryA_G Cuts at: 826 1045 2144 2977 4112 4793 4984 826 Size: 219 1099 833 1135 681 191 1471 Fragments arranged by size: 1471 1135 1099 833 681 219 191 SfoI GGC'GCC Cuts at: 2301 2301 Size: 5629 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2163 2956 1872 Size: 291 793 4545 Fragments arranged by size: 4545 793 291 SmlI C'TyrA_G Cuts at: 820 1017 1943 3733 4634 4896 820 Size: 197 926 1790 901 262 1553 Fragments arranged by size: 1790 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2125 2987 1132 Size: 993 862 3774 Fragments arranged by size: 3774 993 862 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 2543 1517 Size: 1026 4603 StyI C'CwwG_G Cuts at: 513 1065 2484 2824 3068 513 Size: 552 1419 340 244 3074 Fragments arranged by size: 3074 1419 552 340 244 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3656 3673 3826 4011 4456 325 Size: 1255 2076 17 153 185 445 1498 Fragments arranged by size: 2076 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2508 3875 5587 Size: 274 80 33 51 1443 531 1367 1712 Cuts at: 5587 96 Size: 138 Fragments arranged by size: 1712 1443 1367 531 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2032 2064 5589 5621 2032 Size: 32 3525 32 2040 Fragments arranged by size: 3525 2040 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5629 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2602 2602 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2161 2954 1870 Size: 291 793 4545 Fragments arranged by size: 4545 793 291 XmnI GAAnn'nnTTC Cuts at: 2843 3758 2843 Size: 915 4714 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3437 276 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI