Restriction map for
pFN21AB6415
(Linear) (Six-base) MAPSORT of: KIBB6415.seq Check: 6380 from: 1 to: 924
KIBB6415.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 0 425 924
Size: 425 499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 924
Size: 374 550
AfeI AGC'GCT
Cuts at: 0 838 924
Size: 838 86
AhdI GACnn_n'nnGTC
Cuts at: 0 197 924
Size: 197 727
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 536 568 924
Size: 536 32 356
Fragments arranged by size:
536 356 32
AlwNI CAG_nnn'CTG
Cuts at: 0 726 924
Size: 726 198
ApaI G_GGCC'C
Cuts at: 0 91 924
Size: 91 833
ApaLI G'TGCA_C
Cuts at: 0 566 924
Size: 566 358
AvaI C'yCGr_G
Cuts at: 0 156 217 924
Size: 156 61 707
Fragments arranged by size:
707 156 61
BanI G'GyrC_C
Cuts at: 0 160 294 799 924
Size: 160 134 505 125
Fragments arranged by size:
505 160 134 125
BanII G_rGCy'C
Cuts at: 0 91 924
Size: 91 833
BbeI G_GCGC'C
Cuts at: 0 298 924
Size: 298 626
BglI GCCn_nnn'nGGC
Cuts at: 0 71 169 924
Size: 71 98 755
Fragments arranged by size:
755 98 71
BlpI GC'TnA_GC
Cuts at: 0 238 924
Size: 238 686
Bme1580I G_kGCm'C
Cuts at: 0 91 165 374 570 590 924
Size: 91 74 209 196 20 334
Fragments arranged by size:
334 209 196 91 74 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 555 924
Size: 555 369
Bpu10I CC'TnA_GC
Cuts at: 0 243 924
Size: 243 681
BsaI GGTCTCn'nnnn_
Cuts at: 0 642 924
Size: 642 282
BsaHI Gr'CG_yC
Cuts at: 0 295 924
Size: 295 629
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 27 57 924
Size: 27 30 867
Fragments arranged by size:
867 30 27
BseYI C'CCAG_C
Cuts at: 0 288 924
Size: 288 636
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 924
Size: 88 836
BsiEI CG_ry'CG
Cuts at: 0 176 182 924
Size: 176 6 742
Fragments arranged by size:
742 176 6
BsiHKAI G_wGCw'C
Cuts at: 0 570 924
Size: 570 354
Bsp1286I G_dGCh'C
Cuts at: 0 91 165 374 570 590 924
Size: 91 74 209 196 20 334
Fragments arranged by size:
334 209 196 91 74 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 924
Size: 146 778
BsrBI CCG'CTC
Cuts at: 0 28 924
Size: 28 896
BsrDI GCAATG_nn'
Cuts at: 0 703 924
Size: 703 221
BsrFI r'CCGG_y
Cuts at: 0 120 924
Size: 120 804
BssHII G'CGCG_C
Cuts at: 0 322 924
Size: 322 602
BssSI C'ACGA_G
Cuts at: 0 598 629 924
Size: 598 31 295
Fragments arranged by size:
598 295 31
BstXI CCAn_nnnn'nTGG
Cuts at: 0 396 924
Size: 396 528
BtgI C'CryG_G
Cuts at: 0 39 176 479 924
Size: 39 137 303 445
Fragments arranged by size:
445 303 137 39
BtsI GCAGTG_nn'
Cuts at: 0 468 924
Size: 468 456
EaeI y'GGCC_r
Cuts at: 0 173 179 465 476 924
Size: 173 6 286 11 448
Fragments arranged by size:
448 286 173 11 6
EagI C'GGCC_G
Cuts at: 0 173 179 924
Size: 173 6 745
Fragments arranged by size:
745 173 6
EarI CTCTTCn'nnn_
Cuts at: 0 401 924
Size: 401 523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 924
Size: 374 550
EcoO109I rG'GnC_Cy
Cuts at: 0 87 88 191 199 924
Size: 87 1 103 8 725
Fragments arranged by size:
725 103 87 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 543 858 897 924
Size: 543 315 39 27
Fragments arranged by size:
543 315 39 27
FspI TGC'GCA
Cuts at: 0 760 924
Size: 760 164
HaeII r_GCGC'y
Cuts at: 0 66 151 298 317 727 840 924
Size: 66 85 147 19 410 113 84
Fragments arranged by size:
410 147 113 85 84 66 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 183 215 924
Size: 183 32 709
Fragments arranged by size:
709 183 32
KasI G'GCGC_C
Cuts at: 0 294 924
Size: 294 630
MscI TGG'CCA
Cuts at: 0 478 924
Size: 478 446
MslI CAynn'nnrTG
Cuts at: 0 121 377 394 478 598 713 924
Size: 121 256 17 84 120 115 211
Fragments arranged by size:
256 211 121 120 115 84 17
MspA1I CmG'CkG
Cuts at: 0 23 41 102 178 455 558 700 924
Size: 23 18 61 76 277 103 142 224
Fragments arranged by size:
277 224 142 103 76 61 23 18
NarI GG'CG_CC
Cuts at: 0 295 924
Size: 295 629
NcoI C'CATG_G
Cuts at: 0 479 924
Size: 479 445
NotI GC'GGCC_GC
Cuts at: 0 173 924
Size: 173 751
NspI r_CATG'y
Cuts at: 0 120 924
Size: 120 804
PasI CC'CwG_GG
Cuts at: 0 83 195 924
Size: 83 112 729
Fragments arranged by size:
729 112 83
PflMI CCAn_nnn'nTGG
Cuts at: 0 474 924
Size: 474 450
PpuMI rG'GwC_Cy
Cuts at: 0 191 199 924
Size: 191 8 725
Fragments arranged by size:
725 191 8
PspOMI G'GGCC_C
Cuts at: 0 87 924
Size: 87 837
PstI C_TGCA'G
Cuts at: 0 143 924
Size: 143 781
PvuII CAG'CTG
Cuts at: 0 558 924
Size: 558 366
SacII CC_GC'GG
Cuts at: 0 42 179 924
Size: 42 137 745
Fragments arranged by size:
745 137 42
SanDI GG'GwC_CC
Cuts at: 0 191 199 924
Size: 191 8 725
Fragments arranged by size:
725 191 8
SbfI CC_TGCA'GG
Cuts at: 0 143 924
Size: 143 781
SexAI A'CCwGG_T
Cuts at: 0 617 924
Size: 617 307
SfcI C'TryA_G
Cuts at: 0 139 924
Size: 139 785
SfoI GGC'GCC
Cuts at: 0 296 924
Size: 296 628
SmaI CCC'GGG
Cuts at: 0 158 924
Size: 158 766
SphI G_CATG'C
Cuts at: 0 120 924
Size: 120 804
StuI AGG'CCT
Cuts at: 0 538 924
Size: 538 386
StyI C'CwwG_G
Cuts at: 0 479 819 924
Size: 479 340 105
Fragments arranged by size:
479 340 105
TatI w'GTAC_w
Cuts at: 0 503 924
Size: 503 421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 59 924
Size: 27 32 865
Fragments arranged by size:
865 32 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 597 924
Size: 597 327
XmaI C'CCGG_G
Cuts at: 0 156 924
Size: 156 768
XmnI GAAnn'nnTTC
Cuts at: 0 838 924
Size: 838 86
Enzymes that do cut:
AccI AcuI AfeI AhdI AloI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BglI BlpI Bme1580I BplI
Bpu10I BsaI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstXI
BtgI BtsI EaeI EagI EarI Eco57MI EcoO109I FalI
FspI HaeII Hin4I KasI MscI MslI MspA1I NarI
NcoI NotI NspI PasI PflMI PpuMI PspOMI PstI
PvuII SacII SanDI SbfI SexAI SfcI SfoI SmaI
SphI StuI StyI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AleI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI
BmrI BmtI BpmI BpuEI BsaAI BsaBI BsaWI BseRI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
NaeI NdeI NgoMIV NheI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SapI ScaI SfiI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TaqII
Tth111I XbaI XhoI ZraI