(Circular) (Six-base) MAPSORT of: pFN21AE6135.seq Check: 8870 from: 1 to: 5020 pFN21AE6135 5020 bp With 182 enzymes: SgfI * December 8, 2014 13:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2830 278 Size: 53 83 186 936 1294 2468 Fragments arranged by size: 2468 1294 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2363 1937 Size: 426 4594 Acc65I G'GTAC_C Cuts at: 2341 2341 Size: 5020 AclI AA'CG_TT Cuts at: 1563 3147 3520 1563 Size: 1584 373 3063 Fragments arranged by size: 3063 1584 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2207 3083 4467 4735 1632 Size: 575 876 1384 268 1917 Fragments arranged by size: 1917 1384 876 575 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4823 AflIII A'CryG_T Cuts at: 1203 3919 4896 1203 Size: 2716 977 1327 Fragments arranged by size: 2716 1327 977 AgeI A'CCGG_T Cuts at: 3851 3851 Size: 5020 AhdI GACnn_n'nnGTC Cuts at: 3749 3749 Size: 5020 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5020 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5020 AlwNI CAG_nnn'CTG Cuts at: 2763 4335 2763 Size: 1572 3448 ApaI G_GGCC'C Cuts at: 2212 2212 Size: 5020 ApaLI G'TGCA_C Cuts at: 3077 4233 3077 Size: 1156 3864 ApoI r'AATT_y Cuts at: 1493 2216 2329 2555 2707 4816 1493 Size: 723 113 226 152 2109 1697 Fragments arranged by size: 2109 1697 723 226 152 113 AscI GG'CGCG_CC Cuts at: 2170 2170 Size: 5020 AseI AT'TA_AT Cuts at: 160 1032 3574 160 Size: 872 2542 1606 Fragments arranged by size: 2542 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5020 AvaI C'yCGr_G Cuts at: 1870 1943 2345 1870 Size: 73 402 4545 Fragments arranged by size: 4545 402 73 BamHI G'GATC_C Cuts at: 2350 2717 2350 Size: 367 4653 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2341 3796 618 Size: 325 217 627 554 1455 1842 Fragments arranged by size: 1842 1455 627 554 325 217 BanII G_rGCy'C Cuts at: 1391 2212 2339 1391 Size: 821 127 4072 Fragments arranged by size: 4072 821 127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4049 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3211 3245 1903 Size: 34 1274 34 3678 Fragments arranged by size: 3678 1274 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2912 4122 2912 Size: 1210 3810 BclI T'GATC_A Cuts at: 1548 1548 Size: 5020 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3631 136 Size: 107 122 71 631 605 1959 1525 Fragments arranged by size: 1959 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5015 5015 Size: 5020 BlpI GC'TnA_GC Cuts at: 2273 2437 2273 Size: 164 4856 Bme1580I G_kGCm'C Cuts at: 1790 2212 3081 4237 1790 Size: 422 869 1156 2573 Fragments arranged by size: 2573 1156 869 422 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5020 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2751 3709 442 Size: 416 53 83 399 24 1334 958 1753 Fragments arranged by size: 1753 1334 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5020 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3680 1239 Size: 2441 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3145 4010 4308 3145 Size: 865 298 3857 Fragments arranged by size: 3857 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2197 3683 882 Size: 617 698 1486 2219 Fragments arranged by size: 2219 1486 698 617 BsaAI yAC'GTr Cuts at: 493 1204 2811 493 Size: 711 1607 2702 Fragments arranged by size: 2702 1607 711 BsaBI GATnn'nnATC Cuts at: 5014 5014 Size: 5020 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2089 2098 2827 3209 Size: 53 83 186 936 556 9 729 382 Cuts at: 3209 3844 275 Size: 635 1451 Fragments arranged by size: 1451 936 729 635 556 382 186 83 53 9 BsaWI w'CCGG_w Cuts at: 3453 3851 4125 4272 4683 3453 Size: 398 274 147 411 3790 Fragments arranged by size: 3790 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4795 Fragments arranged by size: 4795 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 1186 Size: 1096 3924 BseYI C'CCAG_C Cuts at: 4223 4719 4223 Size: 496 4524 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4447 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2470 3231 4259 1540 Size: 275 186 469 761 1028 2301 Fragments arranged by size: 2301 1028 761 469 275 186 BsiHKAI G_wGCw'C Cuts at: 3081 3166 4237 3081 Size: 85 1071 3864 Fragments arranged by size: 3864 1071 85 BsiWI C'GTAC_G Cuts at: 2160 2160 Size: 5020 BsmI GAATG_Cn' Cuts at: 2531 2624 2531 Size: 93 4927 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2212 2339 3081 3166 4237 1391 Size: 399 422 127 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 422 399 127 85 BspEI T'CCGG_A Cuts at: 4683 4683 Size: 5020 BspHI T'CATG_A Cuts at: 2909 4639 2909 Size: 1730 3290 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2375 844 Size: 1531 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2167 2907 1394 Size: 429 344 740 3507 Fragments arranged by size: 3507 740 429 344 BsrDI GCAATG_nn' Cuts at: 65 1248 3515 3689 65 Size: 1183 2267 174 1396 Fragments arranged by size: 2267 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3664 3851 1700 Size: 110 1854 187 2869 Fragments arranged by size: 2869 1854 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5020 BssHII G'CGCG_C Cuts at: 1925 2170 1925 Size: 245 4775 BssSI C'ACGA_G Cuts at: 3080 4092 3080 Size: 1012 4008 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5020 BstBI TT'CG_AA Cuts at: 3824 3824 Size: 5020 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5020 BstYI r'GATC_y Cuts at: 1972 2350 2717 3102 3119 4571 4657 4669 5015 Size: 378 367 385 17 1452 86 12 346 Cuts at: 5015 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 378 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2092 2175 513 Size: 552 939 24 64 83 3358 Fragments arranged by size: 3358 939 552 83 64 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4281 Fragments arranged by size: 4281 683 56 BtsI GCAGTG_nn' Cuts at: 2536 3330 3350 2536 Size: 794 20 4206 Fragments arranged by size: 4206 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5020 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4985 DraI TTT'AAA Cuts at: 2324 2324 Size: 5020 DraIII CAC_nnn'GTG Cuts at: 2053 2053 Size: 5020 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4027 809 Size: 507 2711 1802 Fragments arranged by size: 2711 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2286 2467 Size: 54 1425 50 275 18 195 261 181 Cuts at: 2467 3356 4985 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 275 261 195 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2467 1537 Size: 275 655 4090 Fragments arranged by size: 4090 655 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2950 1353 Size: 1597 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3607 3981 4127 387 Size: 1064 138 2018 374 146 1280 Fragments arranged by size: 2018 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 3083 3680 4467 4735 1239 Size: 393 575 876 597 787 268 1524 Fragments arranged by size: 1524 876 787 597 575 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2039 2039 Size: 5020 EcoO109I rG'GnC_Cy Cuts at: 2209 2209 Size: 5020 EcoRI G'AATT_C Cuts at: 2329 2707 2329 Size: 378 4642 FspI TGC'GCA Cuts at: 3526 3526 Size: 5020 HaeII r_GCGC'y Cuts at: 1678 4167 1678 Size: 2489 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3661 3693 3735 3767 1373 Size: 32 135 32 2089 32 42 32 2626 Fragments arranged by size: 2626 2089 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2364 2610 669 Size: 988 281 426 246 3079 Fragments arranged by size: 3079 988 426 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5020 HpaI GTT'AAC Cuts at: 2610 2610 Size: 5020 KpnI G_GTAC'C Cuts at: 2345 2345 Size: 5020 MfeI C'AATT_G Cuts at: 2619 2619 Size: 5020 MluI A'CGCG_T Cuts at: 4896 4896 Size: 5020 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4134 4318 1305 Size: 2829 184 2007 Fragments arranged by size: 2829 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3198 Fragments arranged by size: 3198 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2978 3337 3496 518 Size: 648 1812 359 159 2042 Fragments arranged by size: 2042 1812 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2094 2436 3113 4261 4506 1606 Size: 488 342 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 488 342 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2175 513 Size: 552 939 171 3358 Fragments arranged by size: 3358 939 552 171 NdeI CA'TA_TG Cuts at: 387 387 Size: 5020 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5020 NotI GC'GGCC_GC Cuts at: 2467 2467 Size: 5020 NspI r_CATG'y Cuts at: 1132 1229 2378 3923 1132 Size: 97 1149 1545 2229 Fragments arranged by size: 2229 1545 1149 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5020 PciI A'CATG_T Cuts at: 3919 3919 Size: 5020 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5020 PmeI GTTT'AAAC Cuts at: 2324 2324 Size: 5020 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3089 3121 4602 4634 3089 Size: 32 1481 32 3475 Fragments arranged by size: 3475 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5020 PsiI TTA'TAA Cuts at: 2590 2590 Size: 5020 PspOMI G'GGCC_C Cuts at: 2208 2208 Size: 5020 PstI C_TGCA'G Cuts at: 830 2372 830 Size: 1542 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5020 SacII CC_GC'GG Cuts at: 2095 2095 Size: 5020 SalI G'TCGA_C Cuts at: 1936 2362 1936 Size: 426 4594 SbfI CC_TGCA'GG Cuts at: 2372 2372 Size: 5020 ScaI AGT'ACT Cuts at: 3268 4980 3268 Size: 1712 3308 SfcI C'TryA_G Cuts at: 826 1045 2368 3503 4184 4375 826 Size: 219 1323 1135 681 191 1471 Fragments arranged by size: 1471 1323 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5020 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5020 SmaI CCC'GGG Cuts at: 1872 2347 1872 Size: 475 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3124 4025 4287 820 Size: 197 926 1181 901 262 1553 Fragments arranged by size: 1553 1181 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2811 493 Size: 2318 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5020 SphI G_CATG'C Cuts at: 1132 2378 1132 Size: 1246 3774 SspI AAT'ATT Cuts at: 5 52 2944 5 Size: 47 2892 2081 Fragments arranged by size: 2892 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5020 StyI C'CwwG_G Cuts at: 513 1065 2004 2175 2196 2459 513 Size: 552 939 171 21 263 3074 Fragments arranged by size: 3074 939 552 263 171 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3047 3064 3217 3402 3847 325 Size: 1255 1467 17 153 185 445 1498 Fragments arranged by size: 1498 1467 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3266 4978 96 Size: 274 80 33 51 1443 1289 1712 138 Fragments arranged by size: 1712 1443 1289 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4980 5012 4980 Size: 32 4988 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5020 XbaI T'CTAG_A Cuts at: 2356 2356 Size: 5020 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5020 XmaI C'CCGG_G Cuts at: 1870 2345 1870 Size: 475 4545 XmnI GAAnn'nnTTC Cuts at: 3149 3149 Size: 5020 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2828 276 Size: 53 83 186 936 1294 2468 Fragments arranged by size: 2468 1294 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI