Restriction map for
pFN21AE0205
##################################
# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvrII BamHI
BanII BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KpnI
Ksp632I KspI MaeI MfeI MluI
MroI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp45I TspRI Tth111I TthHB8I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcsI 538 GAATTT [GA]^AATT[CT]
AgeI 26 ACCGGT A^CCGGT
AlwI 90 GATCC GGATC(4/5)
ApoI 538 GAATTT [GA]^AATT[CT]
AvaI 380 CCCGGG C^[CT]CG[GA]G
AvaII 384 GGTCC G^G[AT]CC
AviII 455 TGCGCA TGC^GCA
BbeI 5 GGCGCC GGCGC^C
BbvI 174 GCAGC GCAGC(8/12)
BglI 190 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 26 ACCGGT [AT]^CCGG[AT]
BseRI 433 GAGGAG GAGGAG(10/8)
BsgI 634 CTGCAC GTGCAG(16/14)
BsoBI 380 CCCGGG C^[CT]CG[GA]G
BsrFI 26 ACCGGT [GA]^CCGG[CT]
BsrI 602 CCAGT ACTGG(1/-1)
BstYI 89 AGATCC [GA]^GATC[CT]
Cac8I 16 GCTCGC GC[ACGT]^[ACGT]GC
Cfr10I 26 ACCGGT [GA]^CCGG[CT]
DpnII 90 GATC ^GATC
EcoNI 452 CCTTGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 614 CCTTGG C^C[AT][AT]GG
FauI 627 GCGGG CCCGC(4/6)
FspI 455 TGCGCA TGC^GCA
HaeII 5 GGCGCC [GA]GCGC^[CT]
HgaI 482 GACGC GACGC(5/10)
HindIII 421 AAGCTT A^AGCTT
HphI 228 GGTGA GGTGA(8/7)
KasI 5 GGCGCC G^GCGCC
MaeII 596 ACGT A^CGT
MaeIII 23 GTTAC ^GT[ACGT]AC
MboI 90 GATC ^GATC
MflI 89 AGATCC [GA]^GATC[CT]
MseI 527 TTAA T^TAA
MslI 258 CATGCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 175 CAGCTG C[AC]G^C[GT]G
NarI 5 GGCGCC GG^CGCC
NdeII 90 GATC ^GATC
PinAI 26 ACCGGT A^CCGGT
PleI 587 GAGTC GAGTC(4/5)
PvuII 175 CAGCTG CAG^CTG
Sau3AI 90 GATC ^GATC
SfcI 374 CTGTAG C^T[GA][CT]AG
SfoI 5 GGCGCC GGC^GCC
SmaI 380 CCCGGG CCC^GGG
StyI 614 CCTTGG C^C[AT][AT]GG
TfiI 492 GATTC G^A[AT]TC
Tru9I 527 TTAA T^TAA
TseI 174 GCAGC G^C[AT]GC
Tsp509I 539 AATT ^AATT
XcmI 215 CCAACAAATACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 89 AGATCC [GA]^GATC[CT]
XmaCI 380 CCCGGG C^CCGGG
XmaI 380 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 5 GGCGCC G[GA]^CG[CT]C
482 GACGCC
BalI 147 TGGCCA TGG^CCA
554 TGGCCA
BanI 5 GGCGCC G^G[CT][GA]CC
349 GGTGCC
BbsI 544 GAAGAC GAAGAC(2/6)
180 GTCTTC
BcnI 380 CCCGG CC^[GC]GG
381 CCGGG
BpmI 331 CTCCAG CTGGAG(16/14)
193 CTGGAG
BpuAI 544 GAAGAC GAAGAC(2/6)
180 GTCTTC
BsaBI 90 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
492 GATTCATATC
BsaHI 5 GGCGCC G[GA]^CG[CT]C
482 GACGCC
CfoI 6 GCGC GCG^C
456 GCGC
DraIII 140 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
151 CACATGGTG
EaeI 147 TGGCCA [CT]^GGCC[GA]
554 TGGCCA
HapII 27 CCGG C^CGG
381 CCGG
HhaI 6 GCGC GCG^C
456 GCGC
Hin1I 5 GGCGCC G[GA]^CG[CT]C
482 GACGCC
HinP1I 6 GCGC G^CGC
456 GCGC
HinfI 492 GATTC G^A[ACGT]TC
587 GAGTC
HpaII 27 CCGG C^CGG
381 CCGG
MamI 90 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
492 GATTCATATC
MluNI 147 TGGCCA TGG^CCA
554 TGGCCA
MscI 147 TGGCCA TGG^CCA
554 TGGCCA
MspI 27 CCGG C^CGG
381 CCGG
NciI 380 CCCGG CC^[GC]GG
381 CCGGG
NspI 257 ACATGC [GA]CATG^[CT]
438 GCATGT
PflMI 139 CCACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
503 CCAAGAAGTGG
Van91I 139 CCACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
503 CCAAGAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AfaI AluI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cfr13I
EcoRII Fnu4HI FokI HaeIII ItaI
MboII MnlI MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI