(Circular) (Six-base) MAPSORT of: pFN21AE0205.seq Check: 321 from: 1 to: 5347 pFN21AE0205 5347 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3157 278 Size: 53 83 186 936 1621 2468 Fragments arranged by size: 2468 1621 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2690 1937 Size: 753 4594 Acc65I G'GTAC_C Cuts at: 2668 2668 Size: 5347 AclI AA'CG_TT Cuts at: 1563 3474 3847 1563 Size: 1911 373 3063 Fragments arranged by size: 3063 1911 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2211 2414 3410 4794 5062 1632 Size: 579 203 996 1384 268 1917 Fragments arranged by size: 1917 1384 996 579 268 203 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5150 AflIII A'CryG_T Cuts at: 1203 4246 5223 1203 Size: 3043 977 1327 Fragments arranged by size: 3043 1327 977 AgeI A'CCGG_T Cuts at: 2031 4178 2031 Size: 2147 3200 AhdI GACnn_n'nnGTC Cuts at: 4076 4076 Size: 5347 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5347 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5347 AlwNI CAG_nnn'CTG Cuts at: 3090 4662 3090 Size: 1572 3775 ApaLI G'TGCA_C Cuts at: 3404 4560 3404 Size: 1156 4191 ApoI r'AATT_y Cuts at: 1493 2543 2656 2882 3034 5143 1493 Size: 1050 113 226 152 2109 1697 Fragments arranged by size: 2109 1697 1050 226 152 113 AseI AT'TA_AT Cuts at: 160 1032 3901 160 Size: 872 2869 1606 Fragments arranged by size: 2869 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5347 AvaI C'yCGr_G Cuts at: 1870 1943 2385 2672 1870 Size: 73 442 287 4545 Fragments arranged by size: 4545 442 287 73 BamHI G'GATC_C Cuts at: 2677 3044 2677 Size: 367 4980 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2010 2354 2668 4123 618 Size: 325 217 627 223 344 314 1455 1842 Fragments arranged by size: 1842 1455 627 344 325 314 223 217 BanII G_rGCy'C Cuts at: 1391 2666 1391 Size: 1275 4072 BbeI G_GCGC'C Cuts at: 2014 2014 Size: 5347 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2178 2556 928 Size: 971 279 378 3719 Fragments arranged by size: 3719 971 378 279 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3538 3572 1903 Size: 34 1601 34 3678 Fragments arranged by size: 3678 1601 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3239 4449 3239 Size: 1210 4137 BclI T'GATC_A Cuts at: 1548 1548 Size: 5347 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2201 3958 136 Size: 107 122 71 631 605 529 1757 1525 Fragments arranged by size: 1757 1525 631 605 529 122 107 71 BglII A'GATC_T Cuts at: 5342 5342 Size: 5347 BlpI GC'TnA_GC Cuts at: 2764 2764 Size: 5347 Bme1580I G_kGCm'C Cuts at: 1790 3408 4564 1790 Size: 1618 1156 2573 Fragments arranged by size: 2573 1618 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5347 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2601 3078 4036 Size: 416 53 83 399 24 1184 477 958 Cuts at: 4036 442 Size: 1753 Fragments arranged by size: 1753 1184 958 477 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5347 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2291 2291 Size: 5347 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2321 4007 1239 Size: 980 102 1686 2579 Fragments arranged by size: 2579 1686 980 102 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3472 4337 4635 3472 Size: 865 298 4184 Fragments arranged by size: 4184 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4010 882 Size: 617 2511 2219 Fragments arranged by size: 2511 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3138 493 Size: 711 1934 2702 Fragments arranged by size: 2702 1934 711 BsaBI GATnn'nnATC Cuts at: 2099 2501 5341 2099 Size: 402 2840 2105 Fragments arranged by size: 2840 2105 402 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2011 2488 3154 3536 Size: 53 83 186 936 478 477 666 382 Cuts at: 3536 4171 275 Size: 635 1451 Fragments arranged by size: 1451 936 666 635 478 477 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2031 3780 4178 4452 4599 5010 2031 Size: 1749 398 274 147 411 2368 Fragments arranged by size: 2368 1749 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5122 Fragments arranged by size: 5122 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2453 1186 Size: 1267 4080 BseYI C'CCAG_C Cuts at: 2321 4550 5046 2321 Size: 2229 496 2622 Fragments arranged by size: 2622 2229 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2624 1163 Size: 573 888 3886 Fragments arranged by size: 3886 888 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2797 3558 4586 1540 Size: 275 186 796 761 1028 2301 Fragments arranged by size: 2301 1028 796 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3408 3493 4564 3408 Size: 85 1071 4191 Fragments arranged by size: 4191 1071 85 BsmI GAATG_Cn' Cuts at: 2858 2951 2858 Size: 93 5254 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2666 3408 3493 4564 1391 Size: 399 876 742 85 1071 2174 Fragments arranged by size: 2174 1071 876 742 399 85 BspEI T'CCGG_A Cuts at: 5010 5010 Size: 5347 BspHI T'CATG_A Cuts at: 3236 4966 3236 Size: 1730 3617 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2702 844 Size: 1858 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3234 1394 Size: 429 1411 3507 Fragments arranged by size: 3507 1411 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3842 4016 65 Size: 1183 2594 174 1396 Fragments arranged by size: 2594 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 3991 4178 1700 Size: 110 221 1960 187 2869 Fragments arranged by size: 2869 1960 221 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5347 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5347 BssSI C'ACGA_G Cuts at: 3407 4419 3407 Size: 1012 4335 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5347 BstBI TT'CG_AA Cuts at: 4151 4151 Size: 5347 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5347 BstYI r'GATC_y Cuts at: 1972 2094 2677 3044 3429 3446 4898 4984 4996 Size: 122 583 367 385 17 1452 86 12 Cuts at: 4996 5342 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 583 385 367 346 122 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3856 Fragments arranged by size: 3856 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4608 Fragments arranged by size: 4608 683 56 BtsI GCAGTG_nn' Cuts at: 2863 3657 3677 2863 Size: 794 20 4533 Fragments arranged by size: 4533 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5347 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5312 DraI TTT'AAA Cuts at: 2651 2651 Size: 5347 DraIII CAC_nnn'GTG Cuts at: 2150 2161 2150 Size: 11 5336 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4354 809 Size: 507 3038 1802 Fragments arranged by size: 3038 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2152 2559 2794 Size: 54 1425 50 275 18 322 407 235 Cuts at: 2794 3683 5312 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 407 322 275 235 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2794 1537 Size: 275 982 4090 Fragments arranged by size: 4090 982 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3277 1353 Size: 1924 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3934 4308 4454 387 Size: 1064 138 2345 374 146 1280 Fragments arranged by size: 2345 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 2219 2321 2414 3410 4007 4794 Size: 393 579 8 102 93 996 597 787 Cuts at: 4794 5062 1239 Size: 268 1524 Fragments arranged by size: 1524 996 787 597 579 393 268 102 93 8 EcoNI CCTnn'n_nnAGG Cuts at: 2461 2461 Size: 5347 EcoRI G'AATT_C Cuts at: 2656 3034 2656 Size: 378 4969 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2536 2536 Size: 5347 FspI TGC'GCA Cuts at: 2462 3853 2462 Size: 1391 3956 HaeII r_GCGC'y Cuts at: 1678 2014 4494 1678 Size: 336 2480 2531 Fragments arranged by size: 2531 2480 336 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2168 2200 3988 4020 4062 Size: 32 135 32 596 32 1788 32 42 Cuts at: 4062 4094 1373 Size: 32 2626 Fragments arranged by size: 2626 1788 596 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2691 2937 669 Size: 988 281 753 246 3079 Fragments arranged by size: 3079 988 753 281 246 HindIII A'AGCT_T Cuts at: 748 2426 748 Size: 1678 3669 HpaI GTT'AAC Cuts at: 2937 2937 Size: 5347 KasI G'GCGC_C Cuts at: 2010 2010 Size: 5347 KpnI G_GTAC'C Cuts at: 2672 2672 Size: 5347 MfeI C'AATT_G Cuts at: 2946 2946 Size: 5347 MluI A'CGCG_T Cuts at: 5223 5223 Size: 5347 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4461 4645 1305 Size: 3156 184 2007 Fragments arranged by size: 3156 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2154 2561 10 Size: 54 1425 343 322 407 2796 Fragments arranged by size: 2796 1425 407 343 322 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 3305 3664 3823 518 Size: 648 1101 1038 359 159 2042 Fragments arranged by size: 2042 1101 1038 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2182 2763 3440 4588 4833 1606 Size: 576 581 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 581 576 245 NarI GG'CG_CC Cuts at: 2011 2011 Size: 5347 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3856 Fragments arranged by size: 3856 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5347 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5347 NotI GC'GGCC_GC Cuts at: 2794 2794 Size: 5347 NspI r_CATG'y Cuts at: 1132 1229 2266 2447 2705 4250 1132 Size: 97 1037 181 258 1545 2229 Fragments arranged by size: 2229 1545 1037 258 181 97 PasI CC'CwG_GG Cuts at: 1348 2371 1348 Size: 1023 4324 PciI A'CATG_T Cuts at: 4246 4246 Size: 5347 PflMI CCAn_nnn'nTGG Cuts at: 1108 2150 2514 1108 Size: 1042 364 3941 Fragments arranged by size: 3941 1042 364 PmeI GTTT'AAAC Cuts at: 2651 2651 Size: 5347 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3416 3448 4929 4961 3416 Size: 32 1481 32 3802 Fragments arranged by size: 3802 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5347 PsiI TTA'TAA Cuts at: 2917 2917 Size: 5347 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2036 2068 2036 Size: 32 5315 PstI C_TGCA'G Cuts at: 830 2699 830 Size: 1869 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5347 PvuII CAG'CTG Cuts at: 2182 2182 Size: 5347 SalI G'TCGA_C Cuts at: 1936 2689 1936 Size: 753 4594 SbfI CC_TGCA'GG Cuts at: 2699 2699 Size: 5347 ScaI AGT'ACT Cuts at: 3595 5307 3595 Size: 1712 3635 SfcI C'TryA_G Cuts at: 826 1045 2379 2695 3830 4511 4702 826 Size: 219 1334 316 1135 681 191 1471 Fragments arranged by size: 1471 1334 1135 681 316 219 191 SfoI GGC'GCC Cuts at: 2012 2012 Size: 5347 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5347 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5347 SmaI CCC'GGG Cuts at: 1872 2387 2674 1872 Size: 515 287 4545 Fragments arranged by size: 4545 515 287 SmlI C'TyrA_G Cuts at: 820 1017 1943 3451 4352 4614 820 Size: 197 926 1508 901 262 1553 Fragments arranged by size: 1553 1508 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3138 493 Size: 2645 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5347 SphI G_CATG'C Cuts at: 1132 2705 1132 Size: 1573 3774 SspI AAT'ATT Cuts at: 5 52 3271 5 Size: 47 3219 2081 Fragments arranged by size: 3219 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5347 StyI C'CwwG_G Cuts at: 513 1065 2004 2619 2786 513 Size: 552 939 615 167 3074 Fragments arranged by size: 3074 939 615 552 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3374 3391 3544 3729 4174 325 Size: 1255 1794 17 153 185 445 1498 Fragments arranged by size: 1794 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3593 5305 96 Size: 274 80 33 51 1443 1616 1712 138 Fragments arranged by size: 1712 1616 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5307 5339 5307 Size: 32 5315 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5347 XbaI T'CTAG_A Cuts at: 2683 2683 Size: 5347 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2227 2227 Size: 5347 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5347 XmaI C'CCGG_G Cuts at: 1870 2385 2672 1870 Size: 515 287 4545 Fragments arranged by size: 4545 515 287 XmnI GAAnn'nnTTC Cuts at: 3476 3476 Size: 5347 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3155 276 Size: 53 83 186 936 1621 2468 Fragments arranged by size: 2468 1621 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI