Restriction map for
pFN21AE0205
(Linear) (Six-base) MAPSORT of: KIEE0205.seq Check: 9191 from: 1 to: 642
KIEE0205.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 409 642
Size: 206 203 233
Fragments arranged by size:
233 206 203
AgeI A'CCGG_T
Cuts at: 0 26 642
Size: 26 616
ApoI r'AATT_y
Cuts at: 0 538 642
Size: 538 104
AvaI C'yCGr_G
Cuts at: 0 380 642
Size: 380 262
BanI G'GyrC_C
Cuts at: 0 5 349 642
Size: 5 344 293
Fragments arranged by size:
344 293 5
BbeI G_GCGC'C
Cuts at: 0 9 642
Size: 9 633
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 551 642
Size: 173 378 91
Fragments arranged by size:
378 173 91
BglI GCCn_nnn'nGGC
Cuts at: 0 196 642
Size: 196 446
BmrI ACTGGGnnnn_n'
Cuts at: 0 596 642
Size: 596 46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 286 642
Size: 286 356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 316 642
Size: 214 102 326
Fragments arranged by size:
326 214 102
BsaBI GATnn'nnATC
Cuts at: 0 94 496 642
Size: 94 402 146
Fragments arranged by size:
402 146 94
BsaHI Gr'CG_yC
Cuts at: 0 6 483 642
Size: 6 477 159
Fragments arranged by size:
477 159 6
BsaWI w'CCGG_w
Cuts at: 0 26 642
Size: 26 616
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 448 642
Size: 448 194
BseYI C'CCAG_C
Cuts at: 0 316 642
Size: 316 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 642
Size: 619 23
BsrFI r'CCGG_y
Cuts at: 0 26 642
Size: 26 616
BstYI r'GATC_y
Cuts at: 0 89 642
Size: 89 553
DraIII CAC_nnn'GTG
Cuts at: 0 145 156 642
Size: 145 11 486
Fragments arranged by size:
486 145 11
EaeI y'GGCC_r
Cuts at: 0 147 554 642
Size: 147 407 88
Fragments arranged by size:
407 147 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 214 316 409 642
Size: 206 8 102 93 233
Fragments arranged by size:
233 206 102 93 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 456 642
Size: 456 186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 531 642
Size: 531 111
FspI TGC'GCA
Cuts at: 0 457 642
Size: 457 185
HaeII r_GCGC'y
Cuts at: 0 9 642
Size: 9 633
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 642
Size: 163 32 447
Fragments arranged by size:
447 163 32
HindIII A'AGCT_T
Cuts at: 0 421 642
Size: 421 221
KasI G'GCGC_C
Cuts at: 0 5 642
Size: 5 637
MscI TGG'CCA
Cuts at: 0 149 556 642
Size: 149 407 86
Fragments arranged by size:
407 149 86
MslI CAynn'nnrTG
Cuts at: 0 262 642
Size: 262 380
MspA1I CmG'CkG
Cuts at: 0 177 642
Size: 177 465
NarI GG'CG_CC
Cuts at: 0 6 642
Size: 6 636
NspI r_CATG'y
Cuts at: 0 261 442 642
Size: 261 181 200
Fragments arranged by size:
261 200 181
PasI CC'CwG_GG
Cuts at: 0 366 642
Size: 366 276
PflMI CCAn_nnn'nTGG
Cuts at: 0 145 509 642
Size: 145 364 133
Fragments arranged by size:
364 145 133
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 31 63 642
Size: 31 32 579
Fragments arranged by size:
579 32 31
PvuII CAG'CTG
Cuts at: 0 177 642
Size: 177 465
SfcI C'TryA_G
Cuts at: 0 374 642
Size: 374 268
SfoI GGC'GCC
Cuts at: 0 7 642
Size: 7 635
SmaI CCC'GGG
Cuts at: 0 382 642
Size: 382 260
StyI C'CwwG_G
Cuts at: 0 614 642
Size: 614 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 222 642
Size: 222 420
XmaI C'CCGG_G
Cuts at: 0 380 642
Size: 380 262
Enzymes that do cut:
AcuI AgeI ApoI AvaI BanI BbeI BbsI BglI
BmrI BplI BpmI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsrFI BstYI DraIII EaeI Eco57MI EcoNI FalI
FspI HaeII Hin4I HindIII KasI MscI MslI MspA1I
NarI NspI PasI PflMI PsrI PvuII SfcI SfoI
SmaI StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI
Bpu10I BpuEI BsaI BsaAI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EagI EarI EciI EcoICRI EcoO109I EcoRI EcoRV FseI
FspAI HincII HpaI KpnI MfeI MluI MmeI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmnI ZraI