(Circular) (Six-base) MAPSORT of: pFN21AE1063.seq Check: 5435 from: 1 to: 6448 pFN21AE1063 6448 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2231 2231 Size: 6448 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4258 278 Size: 53 83 186 936 2722 2468 Fragments arranged by size: 2722 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3791 1937 Size: 1854 4594 Acc65I G'GTAC_C Cuts at: 3769 3769 Size: 6448 AclI AA'CG_TT Cuts at: 1563 4575 4948 1563 Size: 3012 373 3063 Fragments arranged by size: 3063 3012 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2161 2369 2770 3169 3505 3589 4511 5895 Size: 529 208 401 399 336 84 922 1384 Cuts at: 5895 6163 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 922 529 401 399 336 268 208 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6251 AflIII A'CryG_T Cuts at: 1203 2040 5347 6324 1203 Size: 837 3307 977 1327 Fragments arranged by size: 3307 1327 977 837 AgeI A'CCGG_T Cuts at: 5279 5279 Size: 6448 AhdI GACnn_n'nnGTC Cuts at: 5177 5177 Size: 6448 AleI CACnn'nnGTG Cuts at: 1166 3063 1166 Size: 1897 4551 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6448 AlwNI CAG_nnn'CTG Cuts at: 2077 3675 4191 5763 2077 Size: 1598 516 1572 2762 Fragments arranged by size: 2762 1598 1572 516 ApaLI G'TGCA_C Cuts at: 4505 5661 4505 Size: 1156 5292 ApoI r'AATT_y Cuts at: 1493 2309 3054 3219 3471 3555 3757 3983 4135 Size: 816 745 165 252 84 202 226 152 Cuts at: 4135 6244 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 816 745 252 226 202 165 152 84 AseI AT'TA_AT Cuts at: 160 1032 5002 160 Size: 872 3970 1606 Fragments arranged by size: 3970 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6448 AvaI C'yCGr_G Cuts at: 1870 1943 3773 1870 Size: 73 1830 4545 Fragments arranged by size: 4545 1830 73 BamHI G'GATC_C Cuts at: 3778 4145 3778 Size: 367 6081 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3769 5224 618 Size: 325 217 627 1982 1455 1842 Fragments arranged by size: 1982 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3767 1391 Size: 2376 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3269 928 Size: 971 1370 4107 Fragments arranged by size: 4107 1370 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4639 4673 1903 Size: 34 2702 34 3678 Fragments arranged by size: 3678 2702 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4340 5550 4340 Size: 1210 5238 BclI T'GATC_A Cuts at: 1548 1548 Size: 6448 BfrBI ATG'CAT Cuts at: 3391 3391 Size: 6448 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5059 136 Size: 107 122 71 631 605 3387 1525 Fragments arranged by size: 3387 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6443 6443 Size: 6448 BlpI GC'TnA_GC Cuts at: 3865 3865 Size: 6448 Bme1580I G_kGCm'C Cuts at: 1790 4509 5665 1790 Size: 2719 1156 2573 Fragments arranged by size: 2719 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2041 1318 Size: 723 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2670 3108 4179 Size: 416 53 83 399 24 1253 438 1071 Cuts at: 4179 5137 442 Size: 958 1753 Fragments arranged by size: 1753 1253 1071 958 438 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6448 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 5108 1239 Size: 842 3027 2579 Fragments arranged by size: 3027 2579 842 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2138 4573 5438 5736 2138 Size: 2435 865 298 2850 Fragments arranged by size: 2850 2435 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3481 5111 882 Size: 617 1982 1630 2219 Fragments arranged by size: 2219 1982 1630 617 BsaAI yAC'GTr Cuts at: 493 1204 4239 493 Size: 711 3035 2702 Fragments arranged by size: 3035 2702 711 BsaBI GATnn'nnATC Cuts at: 6442 6442 Size: 6448 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4255 4637 5272 275 Size: 53 83 186 936 2722 382 635 1451 Fragments arranged by size: 2722 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2744 4881 5279 5553 5700 6111 2744 Size: 2137 398 274 147 411 3081 Fragments arranged by size: 3081 2137 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2415 2445 1178 Size: 30 165 30 1012 30 5181 Fragments arranged by size: 5181 1012 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 1186 Size: 892 5556 BseYI C'CCAG_C Cuts at: 5651 6147 5651 Size: 496 5952 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2259 3100 1163 Size: 573 523 841 4511 Fragments arranged by size: 4511 841 573 523 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3898 4659 5687 1540 Size: 275 186 1897 761 1028 2301 Fragments arranged by size: 2301 1897 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4509 4594 5665 4509 Size: 85 1071 5292 Fragments arranged by size: 5292 1071 85 BsmI GAATG_Cn' Cuts at: 2327 2768 2852 3335 3393 3503 3671 3686 3959 Size: 441 84 483 58 110 168 15 273 Cuts at: 3959 4052 2327 Size: 93 4723 Fragments arranged by size: 4723 483 441 273 168 110 93 84 58 15 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3767 4509 4594 5665 1391 Size: 399 1977 742 85 1071 2174 Fragments arranged by size: 2174 1977 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6111 6111 Size: 6448 BspHI T'CATG_A Cuts at: 4337 6067 4337 Size: 1730 4718 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2231 2584 3803 844 Size: 1387 353 1219 3489 Fragments arranged by size: 3489 1387 1219 353 BsrBI CCG'CTC Cuts at: 1394 1823 4335 1394 Size: 429 2512 3507 Fragments arranged by size: 3507 2512 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4943 5117 65 Size: 1183 3695 174 1396 Fragments arranged by size: 3695 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5092 5279 1700 Size: 110 3282 187 2869 Fragments arranged by size: 3282 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2725 3376 96 Size: 2629 651 3168 Fragments arranged by size: 3168 2629 651 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6448 BssSI C'ACGA_G Cuts at: 4508 5520 4508 Size: 1012 5436 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6448 BstBI TT'CG_AA Cuts at: 5252 5252 Size: 6448 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6448 BstXI CCAn_nnnn'nTGG Cuts at: 2262 2262 Size: 6448 BstYI r'GATC_y Cuts at: 1972 3778 4145 4530 4547 5999 6085 6097 6443 Size: 1806 367 385 17 1452 86 12 346 Cuts at: 6443 1972 Size: 1977 Fragments arranged by size: 1977 1806 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2142 513 Size: 552 939 138 4819 Fragments arranged by size: 4819 939 552 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5709 Fragments arranged by size: 5709 683 56 BtsI GCAGTG_nn' Cuts at: 2857 3088 3964 4758 4778 2857 Size: 231 876 794 20 4527 Fragments arranged by size: 4527 876 794 231 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6448 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3406 3441 529 Size: 35 2842 35 3536 Fragments arranged by size: 3536 2842 35 35 DraI TTT'AAA Cuts at: 2475 3752 2475 Size: 1277 5171 DraIII CAC_nnn'GTG Cuts at: 2137 2137 Size: 6448 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5455 809 Size: 507 4139 1802 Fragments arranged by size: 4139 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3895 4784 Size: 54 1425 50 275 18 177 1888 889 Cuts at: 4784 6413 8 Size: 1629 43 Fragments arranged by size: 1888 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3895 1537 Size: 275 2083 4090 Fragments arranged by size: 4090 2083 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4378 1353 Size: 3025 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5035 5409 5555 387 Size: 1064 138 3446 374 146 1280 Fragments arranged by size: 3446 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2161 2369 2770 3169 3505 3589 Size: 393 449 80 208 401 399 336 84 Cuts at: 3589 4511 5108 5895 6163 1239 Size: 922 597 787 268 1524 Fragments arranged by size: 1524 922 787 597 449 401 399 393 336 268 208 84 80 EcoNI CCTnn'n_nnAGG Cuts at: 3523 3523 Size: 6448 EcoO109I rG'GnC_Cy Cuts at: 2220 2220 Size: 6448 EcoRI G'AATT_C Cuts at: 3054 3219 3471 3555 3757 4135 3054 Size: 165 252 84 202 378 5367 Fragments arranged by size: 5367 378 252 202 165 84 FspI TGC'GCA Cuts at: 4954 4954 Size: 6448 HaeII r_GCGC'y Cuts at: 1678 5595 1678 Size: 3917 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2881 2913 2950 2982 3448 Size: 32 135 32 1309 32 37 32 466 Cuts at: 3448 3480 5089 5121 5163 5195 1373 Size: 32 1609 32 42 32 2626 Fragments arranged by size: 2626 1609 1309 466 135 42 37 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2272 2799 2883 3366 3534 3702 Size: 988 281 334 527 84 483 168 168 Cuts at: 3702 3792 4038 669 Size: 90 246 3079 Fragments arranged by size: 3079 988 527 483 334 281 246 168 168 90 84 HindIII A'AGCT_T Cuts at: 748 3181 748 Size: 2433 4015 HpaI GTT'AAC Cuts at: 4038 4038 Size: 6448 KpnI G_GTAC'C Cuts at: 3773 3773 Size: 6448 MfeI C'AATT_G Cuts at: 4047 4047 Size: 6448 MluI A'CGCG_T Cuts at: 6324 6324 Size: 6448 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5562 5746 1305 Size: 4257 184 2007 Fragments arranged by size: 4257 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4449 Fragments arranged by size: 4449 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2929 3063 4406 4765 4924 518 Size: 648 843 920 134 1343 359 159 2042 Fragments arranged by size: 2042 1343 920 843 648 359 159 134 MspA1I CmG'CkG Cuts at: 1606 3864 4541 5689 5934 1606 Size: 2258 677 1148 245 2120 Fragments arranged by size: 2258 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4957 Fragments arranged by size: 4957 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6448 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6448 NotI GC'GGCC_GC Cuts at: 3895 3895 Size: 6448 NsiI A_TGCA'T Cuts at: 3393 3393 Size: 6448 NspI r_CATG'y Cuts at: 1132 1229 3806 5351 1132 Size: 97 2577 1545 2229 Fragments arranged by size: 2577 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6448 PciI A'CATG_T Cuts at: 5347 5347 Size: 6448 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6448 PfoI T'CCnGG_A Cuts at: 2201 2201 Size: 6448 PmeI GTTT'AAAC Cuts at: 3752 3752 Size: 6448 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4517 4549 6030 6062 4517 Size: 32 1481 32 4903 Fragments arranged by size: 4903 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2220 2220 Size: 6448 PshAI GACnn'nnGTC Cuts at: 1613 2940 1613 Size: 1327 5121 PsiI TTA'TAA Cuts at: 2556 3012 4018 2556 Size: 456 1006 4986 Fragments arranged by size: 4986 1006 456 PstI C_TGCA'G Cuts at: 830 2581 3800 830 Size: 1751 1219 3478 Fragments arranged by size: 3478 1751 1219 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6448 SalI G'TCGA_C Cuts at: 1936 3790 1936 Size: 1854 4594 SanDI GG'GwC_CC Cuts at: 2220 2220 Size: 6448 SbfI CC_TGCA'GG Cuts at: 3800 3800 Size: 6448 ScaI AGT'ACT Cuts at: 4696 6408 4696 Size: 1712 4736 SexAI A'CCwGG_T Cuts at: 2133 2188 2133 Size: 55 6393 SfcI C'TryA_G Cuts at: 826 1045 2101 2125 2577 3796 4931 5612 5803 Size: 219 1056 24 452 1219 1135 681 191 Cuts at: 5803 826 Size: 1471 Fragments arranged by size: 1471 1219 1135 1056 681 452 219 191 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6448 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6448 SmaI CCC'GGG Cuts at: 1872 3775 1872 Size: 1903 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2117 4552 5453 5715 820 Size: 197 926 174 2435 901 262 1553 Fragments arranged by size: 2435 1553 926 901 262 197 174 SnaBI TAC'GTA Cuts at: 493 4239 493 Size: 3746 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6448 SphI G_CATG'C Cuts at: 1132 3806 1132 Size: 2674 3774 SspI AAT'ATT Cuts at: 5 52 2441 4372 5 Size: 47 2389 1931 2081 Fragments arranged by size: 2389 2081 1931 47 StuI AGG'CCT Cuts at: 1517 3602 1517 Size: 2085 4363 StyI C'CwwG_G Cuts at: 513 1065 2004 3887 513 Size: 552 939 1883 3074 Fragments arranged by size: 3074 1883 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4475 4492 4645 4830 5275 325 Size: 1255 2895 17 153 185 445 1498 Fragments arranged by size: 2895 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2681 2725 3026 Size: 274 80 33 51 1443 704 44 301 Cuts at: 3026 3376 4694 6406 96 Size: 350 1318 1712 138 Fragments arranged by size: 1712 1443 1318 704 350 301 274 138 80 51 44 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2123 2155 6408 6440 2123 Size: 32 4253 32 2131 Fragments arranged by size: 4253 2131 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6448 XbaI T'CTAG_A Cuts at: 3784 3784 Size: 6448 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6448 XmaI C'CCGG_G Cuts at: 1870 3773 1870 Size: 1903 4545 XmnI GAAnn'nnTTC Cuts at: 3182 3266 3602 3686 4577 3182 Size: 84 336 84 891 5053 Fragments arranged by size: 5053 891 336 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4256 276 Size: 53 83 186 936 2722 2468 Fragments arranged by size: 2722 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI