(Circular) (Six-base) MAPSORT of: pFN21AB8929.seq Check: 6704 from: 1 to: 5548 pFN21AB8929 5548 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2475 2475 Size: 5548 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3358 278 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2891 1937 Size: 954 4594 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 3675 4048 1563 Size: 2112 373 3063 Fragments arranged by size: 3063 2112 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2576 3611 4995 5263 1632 Size: 944 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1035 944 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 2100 4447 5424 1203 Size: 897 2347 977 1327 Fragments arranged by size: 2347 1327 977 897 AgeI A'CCGG_T Cuts at: 4379 4379 Size: 5548 AhdI GACnn_n'nnGTC Cuts at: 4277 4277 Size: 5548 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2557 1184 Size: 1373 4175 AlwNI CAG_nnn'CTG Cuts at: 2195 2500 3291 4863 2195 Size: 305 791 1572 2880 Fragments arranged by size: 2880 1572 791 305 ApaI G_GGCC'C Cuts at: 2085 2219 2085 Size: 134 5414 ApaLI G'TGCA_C Cuts at: 3605 4761 3605 Size: 1156 4392 ApoI r'AATT_y Cuts at: 1493 2857 3083 3235 5344 1493 Size: 1364 226 152 2109 1697 Fragments arranged by size: 2109 1697 1364 226 152 AseI AT'TA_AT Cuts at: 160 1032 4102 160 Size: 872 3070 1606 Fragments arranged by size: 3070 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2037 2057 2084 2376 2873 1870 Size: 73 94 20 27 292 497 4545 Fragments arranged by size: 4545 497 292 94 73 27 20 BamHI G'GATC_C Cuts at: 2878 3245 2878 Size: 367 5181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2560 2869 4324 618 Size: 325 217 627 773 309 1455 1842 Fragments arranged by size: 1842 1455 773 627 325 309 217 BanII G_rGCy'C Cuts at: 1391 2037 2085 2219 2376 2867 1391 Size: 646 48 134 157 491 4072 Fragments arranged by size: 4072 646 491 157 134 48 BbeI G_GCGC'C Cuts at: 2564 2564 Size: 5548 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4577 BbvCI CC'TCA_GC Cuts at: 2630 2630 Size: 5548 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2159 2193 2632 2666 3739 3773 1903 Size: 34 222 34 439 34 1073 34 3678 Fragments arranged by size: 3678 1073 439 222 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3440 4650 3440 Size: 1210 4338 BclI T'GATC_A Cuts at: 1548 1548 Size: 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2214 2380 2522 Size: 107 122 71 631 605 542 166 142 Cuts at: 2522 4159 136 Size: 1637 1525 Fragments arranged by size: 1637 1525 631 605 542 166 142 122 107 71 BglII A'GATC_T Cuts at: 5543 5543 Size: 5548 BlpI GC'TnA_GC Cuts at: 2965 2965 Size: 5548 Bme1580I G_kGCm'C Cuts at: 1790 2085 2219 3609 4765 1790 Size: 295 134 1390 1156 2573 Fragments arranged by size: 2573 1390 1156 295 134 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5548 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3279 4237 442 Size: 416 53 83 399 24 1862 958 1753 Fragments arranged by size: 1862 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2714 2735 4208 1239 Size: 1475 21 1473 2579 Fragments arranged by size: 2579 1475 1473 21 Bpu10I CC'TnA_GC Cuts at: 2630 2630 Size: 5548 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 2495 2639 3673 4538 4836 2195 Size: 300 144 1034 865 298 2907 Fragments arranged by size: 2907 1034 865 300 298 144 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4211 882 Size: 617 2712 2219 Fragments arranged by size: 2712 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3339 493 Size: 711 2135 2702 Fragments arranged by size: 2702 2135 711 BsaBI GATnn'nnATC Cuts at: 5542 5542 Size: 5548 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2561 3355 3737 4372 Size: 53 83 186 936 1028 794 382 635 Cuts at: 4372 275 Size: 1451 Fragments arranged by size: 1451 1028 936 794 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3981 4379 4653 4800 5211 3981 Size: 398 274 147 411 4318 Fragments arranged by size: 4318 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5323 Fragments arranged by size: 5323 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2384 1186 Size: 1198 4350 BseYI C'CCAG_C Cuts at: 2078 2308 4751 5247 2078 Size: 230 2443 496 2379 Fragments arranged by size: 2443 2379 496 230 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4975 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2998 3759 4787 1540 Size: 275 186 997 761 1028 2301 Fragments arranged by size: 2301 1028 997 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3609 3694 4765 3609 Size: 85 1071 4392 Fragments arranged by size: 4392 1071 85 BsmI GAATG_Cn' Cuts at: 2688 3059 3152 2688 Size: 371 93 5084 Fragments arranged by size: 5084 371 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2690 2690 Size: 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2085 2219 2376 2867 3609 3694 Size: 399 247 48 134 157 491 742 85 Cuts at: 3694 4765 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 491 399 247 157 134 85 48 BspEI T'CCGG_A Cuts at: 5211 5211 Size: 5548 BspHI T'CATG_A Cuts at: 3437 5167 3437 Size: 1730 3818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2475 2903 844 Size: 1631 428 3489 Fragments arranged by size: 3489 1631 428 BsrBI CCG'CTC Cuts at: 1394 1823 3435 1394 Size: 429 1612 3507 Fragments arranged by size: 3507 1612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4043 4217 65 Size: 1183 2795 174 1396 Fragments arranged by size: 2795 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2625 4192 4379 1700 Size: 110 815 1567 187 2869 Fragments arranged by size: 2869 1567 815 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5548 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5548 BssSI C'ACGA_G Cuts at: 3608 4620 3608 Size: 1012 4536 BstAPI GCAn_nnn'nTGC Cuts at: 794 2710 794 Size: 1916 3632 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5548 BstXI CCAn_nnnn'nTGG Cuts at: 2356 2356 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2878 3245 3630 3647 5099 5185 5197 5543 Size: 906 367 385 17 1452 86 12 346 Cuts at: 5543 1972 Size: 1977 Fragments arranged by size: 1977 1452 906 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2196 2808 2196 Size: 612 4936 BtgI C'CryG_G Cuts at: 513 1065 2011 513 Size: 552 946 4050 Fragments arranged by size: 4050 946 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2635 487 Size: 683 56 1409 3400 Fragments arranged by size: 3400 1409 683 56 BtsI GCAGTG_nn' Cuts at: 2526 2607 3064 3858 3878 2526 Size: 81 457 794 20 4196 Fragments arranged by size: 4196 794 457 81 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5548 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5513 DraI TTT'AAA Cuts at: 2852 2852 Size: 5548 DraIII CAC_nnn'GTG Cuts at: 2447 2447 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 2491 2523 Size: 54 1425 50 275 18 214 447 32 Cuts at: 2523 2814 2995 3884 5513 8 Size: 291 181 889 1629 43 Fragments arranged by size: 1629 1425 889 447 291 275 214 181 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2995 1537 Size: 275 1183 4090 Fragments arranged by size: 4090 1183 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2148 2798 3478 1353 Size: 795 650 680 3423 Fragments arranged by size: 3423 795 680 650 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2122 2786 4135 4509 4655 387 Size: 1064 138 533 664 1349 374 146 1280 Fragments arranged by size: 1349 1280 1064 664 533 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2576 2714 2735 3611 4208 4995 5263 Size: 393 944 138 21 876 597 787 268 Cuts at: 5263 1239 Size: 1524 Fragments arranged by size: 1524 944 876 787 597 393 268 138 21 EcoNI CCTnn'n_nnAGG Cuts at: 2555 2825 2555 Size: 270 5278 EcoO109I rG'GnC_Cy Cuts at: 2016 2215 2216 2312 2515 2016 Size: 199 1 96 203 5049 Fragments arranged by size: 5049 203 199 96 1 EcoRI G'AATT_C Cuts at: 2857 3235 2857 Size: 378 5170 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2177 2633 2177 Size: 456 5092 FspI TGC'GCA Cuts at: 2660 4054 2660 Size: 1394 4154 HaeII r_GCGC'y Cuts at: 1678 2195 2309 2564 2740 4695 1678 Size: 517 114 255 176 1955 2531 Fragments arranged by size: 2531 1955 517 255 176 114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373 Size: 32 135 32 2617 32 42 32 2626 Fragments arranged by size: 2626 2617 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 3138 669 Size: 988 281 954 246 3079 Fragments arranged by size: 3079 988 954 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5548 HpaI GTT'AAC Cuts at: 3138 3138 Size: 5548 KasI G'GCGC_C Cuts at: 2560 2560 Size: 5548 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4662 4846 1305 Size: 3357 184 2007 Fragments arranged by size: 3357 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2046 2525 10 Size: 54 1425 343 214 479 3033 Fragments arranged by size: 3033 1425 479 343 214 54 MslI CAynn'nnrTG Cuts at: 518 1166 2654 3506 3865 4024 518 Size: 648 1488 852 359 159 2042 Fragments arranged by size: 2042 1488 852 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2964 3641 4789 5034 1606 Size: 1358 677 1148 245 2120 Fragments arranged by size: 2120 1358 1148 677 245 NarI GG'CG_CC Cuts at: 2561 2561 Size: 5548 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4996 NdeI CA'TA_TG Cuts at: 387 387 Size: 5548 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2995 2995 Size: 5548 NspI r_CATG'y Cuts at: 1132 1229 2104 2906 4451 1132 Size: 97 875 802 1545 2229 Fragments arranged by size: 2229 1545 875 802 97 PasI CC'CwG_GG Cuts at: 1348 2333 2349 1348 Size: 985 16 4547 Fragments arranged by size: 4547 985 16 PciI A'CATG_T Cuts at: 2100 4447 2100 Size: 2347 3201 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5548 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 5130 5162 3617 Size: 32 1481 32 4003 Fragments arranged by size: 4003 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2016 2016 Size: 5548 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5548 PsiI TTA'TAA Cuts at: 3118 3118 Size: 5548 PspOMI G'GGCC_C Cuts at: 2081 2215 2081 Size: 134 5414 PspXI vC'TCGA_Gb Cuts at: 2057 2057 Size: 5548 PstI C_TGCA'G Cuts at: 830 2430 2546 2900 830 Size: 1600 116 354 3478 Fragments arranged by size: 3478 1600 354 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5548 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SapI GCTCTTCn'nnn_ Cuts at: 2148 2148 Size: 5548 SbfI CC_TGCA'GG Cuts at: 2900 2900 Size: 5548 ScaI AGT'ACT Cuts at: 3796 5508 3796 Size: 1712 3836 SfcI C'TryA_G Cuts at: 826 1045 2323 2426 2542 2896 4031 4712 4903 Size: 219 1278 103 116 354 1135 681 191 Cuts at: 4903 826 Size: 1471 Fragments arranged by size: 1471 1278 1135 681 354 219 191 116 103 SfiI GGCCn_nnn'nGGCC Cuts at: 2214 2522 2214 Size: 308 5240 SfoI GGC'GCC Cuts at: 2562 2562 Size: 5548 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5548 SmaI CCC'GGG Cuts at: 1872 2039 2086 2378 2875 1872 Size: 167 47 292 497 4545 Fragments arranged by size: 4545 497 292 167 47 SmlI C'TyrA_G Cuts at: 820 1017 1943 2057 2210 2510 2618 3652 4553 Size: 197 926 114 153 300 108 1034 901 Cuts at: 4553 4815 820 Size: 262 1553 Fragments arranged by size: 1553 1034 926 901 300 262 197 153 114 108 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2906 1132 Size: 1774 3774 SspI AAT'ATT Cuts at: 5 52 3472 5 Size: 47 3420 2081 Fragments arranged by size: 3420 2081 47 StuI AGG'CCT Cuts at: 1517 2117 2208 1517 Size: 600 91 4857 Fragments arranged by size: 4857 600 91 StyI C'CwwG_G Cuts at: 513 1065 2987 513 Size: 552 1922 3074 Fragments arranged by size: 3074 1922 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2780 3575 3592 3745 3930 4375 325 Size: 1255 1200 795 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1200 795 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2718 3794 5506 Size: 274 80 33 51 1443 741 1076 1712 Cuts at: 5506 96 Size: 138 Fragments arranged by size: 1712 1443 1076 741 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5508 5540 5508 Size: 32 5516 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5548 XbaI T'CTAG_A Cuts at: 2884 2884 Size: 5548 XhoI C'TCGA_G Cuts at: 1943 2057 1943 Size: 114 5434 XmaI C'CCGG_G Cuts at: 1870 2037 2084 2376 2873 1870 Size: 167 47 292 497 4545 Fragments arranged by size: 4545 497 292 167 47 XmnI GAAnn'nnTTC Cuts at: 3677 3677 Size: 5548 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3356 276 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI PvuII RsrII SacI SacII SanDI SexAI SrfI SwaI XcmI