Restriction map for
pFN21AB6392
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI Ksp632I
KspI MaeI MaeII MamI MboI
MfeI MflI MluI MroI MslI
MunI MvnI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
Sau3AI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI StuI SwaI Tth111I
XbaI XcmI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 282 GGTACC G^GTACC
AlwNI 109 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 282 GGTACC G^GTACC
AspHI 529 GAGCAC G[AT]GC[AT]^C
AvaI 399 CTCGAG C^[CT]CG[GA]G
BalI 732 TGGCCA TGG^CCA
BanI 282 GGTACC G^G[CT][GA]CC
BcnI 588 CCGGG CC^[GC]GG
BlpI 5 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 529 GAGCAC G[AT]GC[AT]^C
BsoBI 399 CTCGAG C^[CT]CG[GA]G
BssSI 459 CTCGTG CACGAG(-5/-1)
CelII 5 GCTCAGC GC^T[ACGT]AGC
CfoI 617 GCGC GCG^C
DraII 250 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 246 CCACGG C^C[GA][CT]GG
EaeI 732 TGGCCA [CT]^GGCC[GA]
EcoO109I 250 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 806 CCTTGG C^C[AT][AT]GG
HhaI 617 GCGC GCG^C
HinP1I 617 GCGC G^CGC
HinfI 780 GATTC G^A[ACGT]TC
HphI 817 TCACC GGTGA(8/7)
KpnI 282 GGTACC GGTAC^C
MboII 882 GAAGA GAAGA(8/7)
MluNI 732 TGGCCA TGG^CCA
MscI 732 TGGCCA TGG^CCA
NciI 588 CCGGG CC^[GC]GG
PaeR7I 399 CTCGAG C^TCGAG
PflMI 669 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 250 GGGACCC [GA]G^G[AT]CC[CT]
PstI 256 CTGCAG CTGCA^G
SbfI 255 CCTGCAGG CCTGCA^GG
SphI 480 GCATGC GCATG^C
Sse8387I 255 CCTGCAGG CCTGCA^GG
StyI 806 CCTTGG C^C[AT][AT]GG
TfiI 780 GATTC G^A[AT]TC
Tsp45I 404 GTGAC ^GT[GC]AC
Van91I 669 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 399 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 319 AAATTT [GA]^AATT[CT]
451 AAATTC
ApoI 319 AAATTT [GA]^AATT[CT]
451 AAATTC
BanII 3 GGGCTC G[GA]GC[CT]^C
536 GAGCCC
BbvI 183 GCAGC GCAGC(8/12)
614 GCTGC
BsmFI 250 GGGAC GGGAC(10/14)
547 GGGAC
BsmI 139 GAATGC GAATGC(1/-1)
430 GAATGC
BsrDI 801 CATTGC GCAATG(2/0)
605 GCAATG
Fnu4HI 183 GCAGC GC^[ACGT]GC
614 GCTGC
HapII 70 CCGG C^CGG
588 CCGG
HpaII 70 CCGG C^CGG
588 CCGG
ItaI 183 GCAGC GC^[ACGT]GC
614 GCTGC
MseI 316 TTAA T^TAA
569 TTAA
MspA1I 8 CAGCTG C[AC]G^C[GT]G
184 CAGCTG
MspI 70 CCGG C^CGG
588 CCGG
NspI 179 ACATGC [GA]CATG^[CT]
480 GCATGC
PvuII 8 CAGCTG CAG^CTG
184 CAGCTG
SfcI 256 CTGCAG C^T[GA][CT]AG
495 CTGTAG
SmlI 399 CTCGAG C^T[CT][GA]AG
738 CTCAAG
TaqI 400 TCGA T^CGA
599 TCGA
Tru9I 316 TTAA T^TAA
569 TTAA
TseI 183 GCAGC G^C[AT]GC
614 GCTGC
Tsp509I 320 AATT ^AATT
452 AATT
TthHB8I 400 TCGA T^CGA
599 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI AvaII BmyI BpmI
BsaI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
FokI HaeIII MaeIII MnlI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI TspRI