(Circular) (Six-base) MAPSORT of: pFN21AB8920.seq Check: 6689 from: 1 to: 5506 pFN21AB8920 5506 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2325 3316 278 Size: 53 83 186 936 789 991 2468 Fragments arranged by size: 2468 991 936 789 186 83 53 AccI GT'mk_AC Cuts at: 1937 2849 1937 Size: 912 4594 Acc65I G'GTAC_C Cuts at: 2827 2827 Size: 5506 AclI AA'CG_TT Cuts at: 1563 3633 4006 1563 Size: 2070 373 3063 Fragments arranged by size: 3063 2070 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 3569 4953 5221 1632 Size: 410 1527 1384 268 1917 Fragments arranged by size: 1917 1527 1384 410 268 AfeI AGC'GCT Cuts at: 2414 2711 2414 Size: 297 5209 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5309 AflIII A'CryG_T Cuts at: 1203 4405 5382 1203 Size: 3202 977 1327 Fragments arranged by size: 3202 1327 977 AgeI A'CCGG_T Cuts at: 4337 4337 Size: 5506 AhdI GACnn_n'nnGTC Cuts at: 4235 4235 Size: 5506 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5506 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5506 AlwNI CAG_nnn'CTG Cuts at: 2212 3249 4821 2212 Size: 1037 1572 2897 Fragments arranged by size: 2897 1572 1037 ApaI G_GGCC'C Cuts at: 2038 2399 2608 2663 2038 Size: 361 209 55 4881 Fragments arranged by size: 4881 361 209 55 ApaLI G'TGCA_C Cuts at: 3563 4719 3563 Size: 1156 4350 ApoI r'AATT_y Cuts at: 1493 2815 3041 3193 5302 1493 Size: 1322 226 152 2109 1697 Fragments arranged by size: 2109 1697 1322 226 152 AseI AT'TA_AT Cuts at: 160 1032 4060 160 Size: 872 3028 1606 Fragments arranged by size: 3028 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 AvaI C'yCGr_G Cuts at: 1870 1943 2029 2188 2366 2831 1870 Size: 73 86 159 178 465 4545 Fragments arranged by size: 4545 465 178 159 86 73 BamHI G'GATC_C Cuts at: 2836 3203 2836 Size: 367 5139 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2085 2384 2466 2492 2827 Size: 325 217 627 298 299 82 26 335 Cuts at: 2827 4282 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 335 325 299 298 217 82 26 BanII G_rGCy'C Cuts at: 1391 2038 2373 2399 2608 2663 2825 1391 Size: 647 335 26 209 55 162 4072 Fragments arranged by size: 4072 647 335 209 162 55 26 BbeI G_GCGC'C Cuts at: 2470 2496 2470 Size: 26 5480 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2343 928 Size: 971 444 4091 Fragments arranged by size: 4091 971 444 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2567 2601 3697 3731 1903 Size: 34 50 34 546 34 1096 34 3678 Fragments arranged by size: 3678 1096 546 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3398 4608 3398 Size: 1210 4296 BclI T'GATC_A Cuts at: 1548 1548 Size: 5506 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2580 4117 136 Size: 107 122 71 631 605 908 1537 1525 Fragments arranged by size: 1537 1525 908 631 605 122 107 71 BglII A'GATC_T Cuts at: 5501 5501 Size: 5506 BlpI GC'TnA_GC Cuts at: 2923 2923 Size: 5506 Bme1580I G_kGCm'C Cuts at: 1790 2038 2399 2608 2663 3567 4723 1790 Size: 248 361 209 55 904 1156 2573 Fragments arranged by size: 2573 1156 904 361 248 209 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5506 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2465 3237 4195 Size: 416 53 83 399 24 1048 772 958 Cuts at: 4195 442 Size: 1753 Fragments arranged by size: 1753 1048 958 772 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5506 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2341 2341 Size: 5506 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2156 2546 2639 4166 1239 Size: 872 45 390 93 1527 2579 Fragments arranged by size: 2579 1527 872 390 93 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3631 4496 4794 3631 Size: 865 298 4343 Fragments arranged by size: 4343 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4169 882 Size: 617 2670 2219 Fragments arranged by size: 2670 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3297 493 Size: 711 2093 2702 Fragments arranged by size: 2702 2093 711 BsaBI GATnn'nnATC Cuts at: 5500 5500 Size: 5506 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2322 2467 2493 2747 Size: 53 83 186 936 789 145 26 254 Cuts at: 2747 3313 3695 4330 275 Size: 566 382 635 1451 Fragments arranged by size: 1451 936 789 635 566 382 254 186 145 83 53 26 BsaWI w'CCGG_w Cuts at: 3939 4337 4611 4758 5169 3939 Size: 398 274 147 411 4276 Fragments arranged by size: 4276 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5281 Fragments arranged by size: 5281 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5506 BseYI C'CCAG_C Cuts at: 2253 2439 2685 4709 5205 2253 Size: 186 246 2024 496 2554 Fragments arranged by size: 2554 2024 496 246 186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4933 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2172 2269 2511 2643 2956 3717 Size: 275 186 171 97 242 132 313 761 Cuts at: 3717 4745 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 313 275 242 186 171 132 97 BsiHKAI G_wGCw'C Cuts at: 2433 2625 2679 3567 3652 4723 2433 Size: 192 54 888 85 1071 3216 Fragments arranged by size: 3216 1071 888 192 85 54 BsmI GAATG_Cn' Cuts at: 3017 3110 3017 Size: 93 5413 BsmBI CGTCTCn'nnnn_ Cuts at: 2329 2329 Size: 5506 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2373 2399 2433 2608 2625 2663 Size: 399 248 335 26 34 175 17 38 Cuts at: 2663 2679 2825 3567 3652 4723 1391 Size: 16 146 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 335 248 175 146 85 38 34 26 17 16 BspEI T'CCGG_A Cuts at: 5169 5169 Size: 5506 BspHI T'CATG_A Cuts at: 2724 3395 5125 2724 Size: 671 1730 3105 Fragments arranged by size: 3105 1730 671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2861 844 Size: 1295 722 3489 Fragments arranged by size: 3489 1295 722 BsrBI CCG'CTC Cuts at: 1394 1823 2028 3393 1394 Size: 429 205 1365 3507 Fragments arranged by size: 3507 1365 429 205 BsrDI GCAATG_nn' Cuts at: 65 1248 4001 4175 65 Size: 1183 2753 174 1396 Fragments arranged by size: 2753 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2449 4150 4337 1700 Size: 110 639 1701 187 2869 Fragments arranged by size: 2869 1701 639 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5506 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5506 BssSI C'ACGA_G Cuts at: 3566 4578 3566 Size: 1012 4494 BstAPI GCAn_nnn'nTGC Cuts at: 794 2629 794 Size: 1835 3671 BstBI TT'CG_AA Cuts at: 4310 4310 Size: 5506 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5506 BstYI r'GATC_y Cuts at: 1972 2836 3203 3588 3605 5057 5143 5155 5501 Size: 864 367 385 17 1452 86 12 346 Cuts at: 5501 1972 Size: 1977 Fragments arranged by size: 1977 1452 864 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2355 2355 Size: 5506 BtgI C'CryG_G Cuts at: 513 1065 2067 2118 2424 513 Size: 552 1002 51 306 3595 Fragments arranged by size: 3595 1002 552 306 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2394 487 Size: 683 56 1168 3599 Fragments arranged by size: 3599 1168 683 56 BtsI GCAGTG_nn' Cuts at: 3022 3816 3836 3022 Size: 794 20 4692 Fragments arranged by size: 4692 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5506 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5471 DraI TTT'AAA Cuts at: 2810 2810 Size: 5506 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4513 809 Size: 507 3197 1802 Fragments arranged by size: 3197 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2169 2266 2508 Size: 54 1425 50 275 18 339 97 242 Cuts at: 2508 2548 2640 2953 3842 5471 8 Size: 40 92 313 889 1629 43 Fragments arranged by size: 1629 1425 889 339 313 275 242 97 92 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 2169 2266 2508 2640 2953 1537 Size: 275 357 97 242 132 313 4090 Fragments arranged by size: 4090 357 313 275 242 132 97 EarI CTCTTCn'nnn_ Cuts at: 1353 2732 3436 1353 Size: 1379 704 3423 Fragments arranged by size: 3423 1379 704 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4093 4467 4613 387 Size: 1064 138 2504 374 146 1280 Fragments arranged by size: 2504 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2111 2156 2546 2639 3569 4166 Size: 393 410 69 45 390 93 930 597 Cuts at: 4166 4953 5221 1239 Size: 787 268 1524 Fragments arranged by size: 1524 930 787 597 410 393 390 268 93 69 45 EcoO109I rG'GnC_Cy Cuts at: 2034 2035 2110 2604 2659 2660 2034 Size: 1 75 494 55 1 4880 Fragments arranged by size: 4880 494 75 55 1 1 EcoRI G'AATT_C Cuts at: 2815 3193 2815 Size: 378 5128 FspI TGC'GCA Cuts at: 4012 4012 Size: 5506 HaeII r_GCGC'y Cuts at: 1678 2416 2470 2496 2713 4653 1678 Size: 738 54 26 217 1940 2531 Fragments arranged by size: 2531 1940 738 217 54 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2489 2521 4147 4179 4221 Size: 32 135 32 917 32 1626 32 42 Cuts at: 4221 4253 1373 Size: 32 2626 Fragments arranged by size: 2626 1626 917 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2850 3096 669 Size: 988 281 912 246 3079 Fragments arranged by size: 3079 988 912 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5506 HpaI GTT'AAC Cuts at: 3096 3096 Size: 5506 KasI G'GCGC_C Cuts at: 2466 2492 2466 Size: 26 5480 KpnI G_GTAC'C Cuts at: 2831 2831 Size: 5506 MfeI C'AATT_G Cuts at: 3105 3105 Size: 5506 MluI A'CGCG_T Cuts at: 5382 5382 Size: 5506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4620 4804 1305 Size: 3315 184 2007 Fragments arranged by size: 3315 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2550 10 Size: 54 1425 343 718 2966 Fragments arranged by size: 2966 1425 718 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 3464 3823 3982 518 Size: 648 906 1392 359 159 2042 Fragments arranged by size: 2042 1392 906 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2253 2289 2298 2330 2350 2439 2685 2922 Size: 647 36 9 32 20 89 246 237 Cuts at: 2922 3599 4747 4992 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 647 246 245 237 89 36 32 20 9 NarI GG'CG_CC Cuts at: 2467 2493 2467 Size: 26 5480 NcoI C'CATG_G Cuts at: 513 1065 2067 2424 513 Size: 552 1002 357 3595 Fragments arranged by size: 3595 1002 552 357 NdeI CA'TA_TG Cuts at: 387 387 Size: 5506 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5506 NotI GC'GGCC_GC Cuts at: 2953 2953 Size: 5506 NspI r_CATG'y Cuts at: 1132 1229 2864 4409 1132 Size: 97 1635 1545 2229 Fragments arranged by size: 2229 1635 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5506 PciI A'CATG_T Cuts at: 4405 4405 Size: 5506 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5506 PfoI T'CCnGG_A Cuts at: 2559 2559 Size: 5506 PmeI GTTT'AAAC Cuts at: 2810 2810 Size: 5506 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3575 3607 5088 5120 3575 Size: 32 1481 32 3961 Fragments arranged by size: 3961 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2110 2110 Size: 5506 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5506 PsiI TTA'TAA Cuts at: 3076 3076 Size: 5506 PspOMI G'GGCC_C Cuts at: 2034 2395 2604 2659 2034 Size: 361 209 55 4881 Fragments arranged by size: 4881 361 209 55 PstI C_TGCA'G Cuts at: 830 2024 2858 830 Size: 1194 834 3478 Fragments arranged by size: 3478 1194 834 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5506 SalI G'TCGA_C Cuts at: 1936 2848 1936 Size: 912 4594 SapI GCTCTTCn'nnn_ Cuts at: 2732 2732 Size: 5506 SbfI CC_TGCA'GG Cuts at: 2858 2858 Size: 5506 ScaI AGT'ACT Cuts at: 3754 5466 3754 Size: 1712 3794 SfcI C'TryA_G Cuts at: 826 1045 2020 2665 2854 3989 4670 4861 826 Size: 219 975 645 189 1135 681 191 1471 Fragments arranged by size: 1471 1135 975 681 645 219 191 189 SfiI GGCCn_nnn'nGGCC Cuts at: 2580 2580 Size: 5506 SfoI GGC'GCC Cuts at: 2468 2494 2468 Size: 26 5480 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5506 SmaI CCC'GGG Cuts at: 1872 2190 2833 1872 Size: 318 643 4545 Fragments arranged by size: 4545 643 318 SmlI C'TyrA_G Cuts at: 820 1017 1943 3610 4511 4773 820 Size: 197 926 1667 901 262 1553 Fragments arranged by size: 1667 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3297 493 Size: 2804 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5506 SphI G_CATG'C Cuts at: 1132 2864 1132 Size: 1732 3774 SspI AAT'ATT Cuts at: 5 52 3430 5 Size: 47 3378 2081 Fragments arranged by size: 3378 2081 47 StuI AGG'CCT Cuts at: 1517 2063 2516 1517 Size: 546 453 4507 Fragments arranged by size: 4507 546 453 StyI C'CwwG_G Cuts at: 513 1065 2067 2424 2700 2945 513 Size: 552 1002 357 276 245 3074 Fragments arranged by size: 3074 1002 552 357 276 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 2274 3533 3550 3703 3888 4333 Size: 1255 651 43 1259 17 153 185 445 Cuts at: 4333 325 Size: 1498 Fragments arranged by size: 1498 1259 1255 651 445 185 153 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3752 5464 96 Size: 274 80 33 51 1443 1775 1712 138 Fragments arranged by size: 1775 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5466 5498 5466 Size: 32 5474 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5506 XbaI T'CTAG_A Cuts at: 2842 2842 Size: 5506 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5506 XmaI C'CCGG_G Cuts at: 1870 2188 2831 1870 Size: 318 643 4545 Fragments arranged by size: 4545 643 318 XmnI GAAnn'nnTTC Cuts at: 3635 3635 Size: 5506 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2323 3314 276 Size: 53 83 186 936 789 991 2468 Fragments arranged by size: 2468 991 936 789 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SrfI SwaI XcmI