Restriction map for
pFN21AB8237
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AviII AvrII
BamHI BanII BbeI BbvCI BcgI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcyI 570 GACGCC G[GA]^CG[CT]C
AfaI 582 GTAC GT^AC
AhdI 265 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 104 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 265 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 629 CCCGAG C^[CT]CG[GA]G
BalI 485 TGGCCA TGG^CCA
BbrPI 601 CACGTG CAC^GTG
BciVI 697 GGATAC GTATCC(6/5)
BcnI 79 CCCGG CC^[GC]GG
BfaI 262 CTAG C^TAG
BglI 619 GCCTTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 760 GTGCCC G[AGT]GC[ACT]^C
BsaAI 601 CACGTG [CT]AC^GT[GA]
BsaHI 570 GACGCC G[GA]^CG[CT]C
BsaI 409 GAGACC GGTCTC(1/5)
BseRI 85 GAGGAG GAGGAG(10/8)
BsgI 132 CTGCAC GTGCAG(16/14)
BsoBI 629 CCCGAG C^[CT]CG[GA]G
Bsp1286I 760 GTGCCC G[AGT]GC[ACT]^C
BsrI 167 CCAGT ACTGG(1/-1)
DpnII 720 GATC ^GATC
DraII 65 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 686 CCGTGG C^C[GA][CT]GG
Eam1105I 265 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 742 CTCTTC CTCTTC(1/4)
EcoO109I 65 GGGACCC [GA]G^G[ACGT]CC[CT]
HgaI 570 GACGC GACGC(5/10)
Hin1I 570 GACGCC G[GA]^CG[CT]C
HincII 346 GTCAAC GT[CT]^[GA]AC
HindII 346 GTCAAC GT[CT]^[GA]AC
Ksp632I 742 CTCTTC CTCTTC(1/4)
MaeI 262 CTAG C^TAG
MboI 720 GATC ^GATC
MluNI 485 TGGCCA TGG^CCA
MscI 485 TGGCCA TGG^CCA
MslI 669 CACCCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 252 GCCGGC GCC^GGC
NciI 79 CCCGG CC^[GC]GG
NdeII 720 GATC ^GATC
NgoMIV 252 GCCGGC G^CCGGC
PflMI 447 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 601 CACGTG CAC^GTG
PmlI 601 CACGTG CAC^GTG
PpuMI 65 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 310 CAGCTG CAG^CTG
RsaI 582 GTAC GT^AC
SapI 741 GCTCTTC GCTCTTC(1/4)
Sau3AI 720 GATC ^GATC
SexAI 755 ACCTGGT A^CC[AT]GGT
SfaNI 163 GATGC GCATC(5/9)
SfiI 618 GGCCTTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 736 CTCAAG C^T[CT][GA]AG
StuI 617 AGGCCT AGG^CCT
TaqI 722 TCGA T^CGA
TfiI 150 GAATC G^A[AT]TC
TthHB8I 722 TCGA T^CGA
Van91I 447 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 364 CGCG CG^CG
592 CGCG
BanI 436 GGTGCC G^G[CT][GA]CC
759 GGTGCC
BbsI 22 GAAGAC GAAGAC(2/6)
73 GAAGAC
BpmI 427 CTGGAG CTGGAG(16/14)
526 CTGGAG
BpuAI 22 GAAGAC GAAGAC(2/6)
73 GAAGAC
BsaWI 159 ACCGGA [AT]^CCGG[AT]
701 ACCGGA
BsmAI 409 GAGAC GTCTC(1/5)
122 GTCTC
BsmFI 65 GGGAC GGGAC(10/14)
568 GGGAC
BsrFI 221 ACCGGC [GA]^CCGG[CT]
252 GCCGGC
BstUI 364 CGCG CG^CG
592 CGCG
Cfr10I 221 ACCGGC [GA]^CCGG[CT]
252 GCCGGC
DrdI 106 GACACTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
337 GACAACCTCGTC
EaeI 254 CGGCCA [CT]^GGCC[GA]
485 TGGCCA
FauI 763 CCCGC CCCGC(4/6)
593 GCGGG
HaeII 472 AGCGCC [GA]GCGC^[CT]
634 GGCGCT
MaeII 536 ACGT A^CGT
602 ACGT
MaeIII 335 GTGAC ^GT[ACGT]AC
533 GTGAC
MspA1I 310 CAGCTG C[AC]G^C[GT]G
764 CCGCTG
MvnI 364 CGCG CG^CG
592 CGCG
PleI 265 GACTC GAGTC(4/5)
658 GACTC
PstI 17 CTGCAG CTGCA^G
92 CTGCAG
SfcI 17 CTGCAG C^T[GA][CT]AG
92 CTGCAG
Tsp45I 335 GTGAC ^GT[GC]AC
533 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboII MnlI MspI MvaI MwoI
NlaIV Sau96I ScrFI TseI TspRI