Restriction map for
pFN21AB8237
(Linear) (Six-base) MAPSORT of: KIBB8237.seq Check: 4859 from: 1 to: 777
KIBB8237.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 217 334 777
Size: 92 125 117 443
Fragments arranged by size:
443 125 117 92
AhdI GACnn_n'nnGTC
Cuts at: 0 270 777
Size: 270 507
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 65 775 777
Size: 65 710 2
Fragments arranged by size:
710 65 2
AlwNI CAG_nnn'CTG
Cuts at: 0 109 777
Size: 109 668
AvaI C'yCGr_G
Cuts at: 0 629 777
Size: 629 148
BanI G'GyrC_C
Cuts at: 0 436 759 777
Size: 436 323 18
Fragments arranged by size:
436 323 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 29 80 777
Size: 29 51 697
Fragments arranged by size:
697 51 29
BciVI GTATCCnnnnn_n'
Cuts at: 0 691 777
Size: 691 86
BglI GCCn_nnn'nGGC
Cuts at: 0 625 777
Size: 625 152
Bme1580I G_kGCm'C
Cuts at: 0 764 777
Size: 764 13
BmgBI CAC'GTC
Cuts at: 0 537 777
Size: 537 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 547 777
Size: 448 99 230
Fragments arranged by size:
448 230 99
Bpu10I CC'TnA_GC
Cuts at: 0 140 777
Size: 140 637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 777
Size: 721 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 403 777
Size: 403 374
BsaAI yAC'GTr
Cuts at: 0 603 777
Size: 603 174
BsaHI Gr'CG_yC
Cuts at: 0 571 777
Size: 571 206
BsaWI w'CCGG_w
Cuts at: 0 159 701 777
Size: 159 542 76
Fragments arranged by size:
542 159 76
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 777
Size: 100 677
BseYI C'CCAG_C
Cuts at: 0 11 38 777
Size: 11 27 739
Fragments arranged by size:
739 27 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 777
Size: 117 660
Bsp1286I G_dGCh'C
Cuts at: 0 764 777
Size: 764 13
BsrFI r'CCGG_y
Cuts at: 0 221 252 777
Size: 221 31 525
Fragments arranged by size:
525 221 31
BtgI C'CryG_G
Cuts at: 0 686 777
Size: 686 91
BtsI GCAGTG_nn'
Cuts at: 0 574 777
Size: 574 203
DrdI GACnn_nn'nnGTC
Cuts at: 0 112 343 777
Size: 112 231 434
Fragments arranged by size:
434 231 112
EaeI y'GGCC_r
Cuts at: 0 254 485 777
Size: 254 231 292
Fragments arranged by size:
292 254 231
EarI CTCTTCn'nnn_
Cuts at: 0 748 777
Size: 748 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 381 777
Size: 381 396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 217 334 448 547 777
Size: 92 125 117 114 99 230
Fragments arranged by size:
230 125 117 114 99 92
EcoO109I rG'GnC_Cy
Cuts at: 0 66 777
Size: 66 711
HaeII r_GCGC'y
Cuts at: 0 476 638 777
Size: 476 162 139
Fragments arranged by size:
476 162 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 777
Size: 256 32 489
Fragments arranged by size:
489 256 32
HincII GTy'rAC
Cuts at: 0 348 777
Size: 348 429
MscI TGG'CCA
Cuts at: 0 487 777
Size: 487 290
MslI CAynn'nnrTG
Cuts at: 0 673 777
Size: 673 104
MspA1I CmG'CkG
Cuts at: 0 312 766 777
Size: 312 454 11
Fragments arranged by size:
454 312 11
NaeI GCC'GGC
Cuts at: 0 254 777
Size: 254 523
NgoMIV G'CCGG_C
Cuts at: 0 252 777
Size: 252 525
PasI CC'CwG_GG
Cuts at: 0 693 777
Size: 693 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 453 777
Size: 453 324
PmlI CAC'GTG
Cuts at: 0 603 777
Size: 603 174
PpuMI rG'GwC_Cy
Cuts at: 0 66 777
Size: 66 711
PstI C_TGCA'G
Cuts at: 0 21 96 777
Size: 21 75 681
Fragments arranged by size:
681 75 21
PvuII CAG'CTG
Cuts at: 0 312 777
Size: 312 465
SanDI GG'GwC_CC
Cuts at: 0 66 777
Size: 66 711
SapI GCTCTTCn'nnn_
Cuts at: 0 748 777
Size: 748 29
SexAI A'CCwGG_T
Cuts at: 0 755 777
Size: 755 22
SfcI C'TryA_G
Cuts at: 0 17 92 777
Size: 17 75 685
Fragments arranged by size:
685 75 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 625 777
Size: 625 152
SmlI C'TyrA_G
Cuts at: 0 736 777
Size: 736 41
StuI AGG'CCT
Cuts at: 0 619 777
Size: 619 158
Enzymes that do cut:
AcuI AhdI AlfI AlwNI AvaI BanI BbsI BciVI
BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsgI Bsp1286I BsrFI BtgI
BtsI DrdI EaeI EarI EciI Eco57MI EcoO109I HaeII
Hin4I HincII MscI MslI MspA1I NaeI NgoMIV PasI
PflMI PmlI PpuMI PstI PvuII SanDI SapI SexAI
SfcI SfiI SmlI StuI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BclI BfrBI BglII BlpI BmrI BmtI BplI BsaBI
BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EagI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI