Restriction map for
pFN21AB6470
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI AspEI AspHI AspI AvaII
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 630 GGTACC G^GTACC
AccII 27 CGCG CG^CG
AcyI 583 GACGCC G[GA]^CG[CT]C
AflIII 749 ACGTGT A^C[GA][CT]GT
AlwNI 691 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 747 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 630 GGTACC G^GTACC
AvaI 269 CCCGGG C^[CT]CG[GA]G
BalI 1055 TGGCCA TGG^CCA
BanI 630 GGTACC G^G[CT][GA]CC
BbvI 86 GCAGC GCAGC(8/12)
BciVI 961 GGATAC GTATCC(6/5)
BglI 1048 GCCCCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 583 GACGCC G[GA]^CG[CT]C
BsaWI 563 TCCGGT [AT]^CCGG[AT]
BseRI 462 GAGGAG GAGGAG(10/8)
BsgI 554 GTGCAG GTGCAG(16/14)
BsmAI 680 GAGAC GTCTC(1/5)
BsmFI 546 GTCCC GGGAC(10/14)
BsoBI 269 CCCGGG C^[CT]CG[GA]G
BspMI 704 ACCTGC ACCTGC(4/8)
BsrDI 219 CATTGC GCAATG(2/0)
BstAPI 254 GCATTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 420 GGATG GGATG(2/0)
BstUI 27 CGCG CG^CG
BstYI 590 AGATCC [GA]^GATC[CT]
DdeI 720 CTCAG C^T[ACGT]AG
DraIII 805 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 386 CTCTTC CTCTTC(1/4)
EcoRV 880 GATATC GAT^ATC
EcoT14I 470 CCATGG C^C[AT][AT]GG
Fnu4HI 86 GCAGC GC^[ACGT]GC
FokI 420 GGATG GGATG(9/13)
HgaI 583 GACGC GACGC(5/10)
Hin1I 583 GACGCC G[GA]^CG[CT]C
ItaI 86 GCAGC GC^[ACGT]GC
KpnI 630 GGTACC GGTAC^C
Ksp632I 386 CTCTTC CTCTTC(1/4)
MflI 590 AGATCC [GA]^GATC[CT]
MluNI 1055 TGGCCA TGG^CCA
MscI 1055 TGGCCA TGG^CCA
MslI 966 CACTCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 549 CCGCTG C[AC]G^C[GT]G
MvnI 27 CGCG CG^CG
NaeI 91 GCCGGC GCC^GGC
NcoI 470 CCATGG C^CATGG
NgoMIV 91 GCCGGC G^CCGGC
PleI 49 GACTC GAGTC(4/5)
PstI 706 CTGCAG CTGCA^G
SfcI 706 CTGCAG C^T[GA][CT]AG
SfiI 1047 GGCCCCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 269 CCCGGG CCC^GGG
SmlI 459 CTTGAG C^T[CT][GA]AG
StuI 1014 AGGCCT AGG^CCT
StyI 470 CCATGG C^C[AT][AT]GG
TfiI 840 GATTC G^A[AT]TC
TseI 86 GCAGC G^C[AT]GC
XhoII 590 AGATCC [GA]^GATC[CT]
XmaCI 269 CCCGGG C^CCGGG
XmaI 269 CCCGGG C^CCGGG
XmnI 747 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 567 GTAC GT^AC
631 GTAC
AluI 137 AGCT AG^CT
575 AGCT
AlwI 591 GATCC GGATC(4/5)
798 GATCC
BbsI 160 GAAGAC GAAGAC(2/6)
1038 GAAGAC
BfaI 178 CTAG C^TAG
652 CTAG
BpmI 230 CTCCAG CTGGAG(16/14)
940 CTGGAG
BpuAI 160 GAAGAC GAAGAC(2/6)
1038 GAAGAC
BsmI 813 GAATGC GAATGC(1/-1)
254 GCATTC
BsrFI 91 GCCGGC [GA]^CCGG[CT]
238 ACCGGC
Cfr10I 91 GCCGGC [GA]^CCGG[CT]
238 ACCGGC
DsaI 109 CCGTGG C^C[GA][CT]GG
470 CCATGG
EaeI 93 CGGCCA [CT]^GGCC[GA]
1055 TGGCCA
HaeII 88 AGCGCC [GA]GCGC^[CT]
693 GGCGCT
HinfI 49 GACTC G^A[ACGT]TC
840 GATTC
HphI 596 TCACC GGTGA(8/7)
871 TCACC
MaeI 178 CTAG C^TAG
652 CTAG
MaeII 341 ACGT A^CGT
749 ACGT
MaeIII 382 GTCAC ^GT[ACGT]AC
764 GTGAC
MseI 359 TTAA T^TAA
737 TTAA
RsaI 567 GTAC GT^AC
631 GTAC
SfaNI 298 GATGC GCATC(5/9)
858 GCATC
Tru9I 359 TTAA T^TAA
737 TTAA
Tsp45I 382 GTCAC ^GT[GC]AC
764 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI ApaI BanII BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DpnII DraII EcoO109I EcoRII FauI
HaeIII HapII HhaI HinP1I HpaII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TaqI Tsp509I
TspRI TthHB8I