(Circular) (Six-base) MAPSORT of: pFN21AB6470.seq Check: 6824 from: 1 to: 5767 pFN21AB6470 5767 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3577 278 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3110 1937 Size: 1173 4594 Acc65I G'GTAC_C Cuts at: 2635 3088 2635 Size: 453 5314 AclI AA'CG_TT Cuts at: 1563 3894 4267 1563 Size: 2331 373 3063 Fragments arranged by size: 3063 2331 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2059 3830 5214 5482 1632 Size: 427 1771 1384 268 1917 Fragments arranged by size: 1917 1771 1384 427 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 2754 4666 5643 1203 Size: 1551 1912 977 1327 Fragments arranged by size: 1912 1551 1327 977 AgeI A'CCGG_T Cuts at: 4598 4598 Size: 5767 AhdI GACnn_n'nnGTC Cuts at: 4496 4496 Size: 5767 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5767 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5767 AlwNI CAG_nnn'CTG Cuts at: 2701 3510 5082 2701 Size: 809 1572 3386 Fragments arranged by size: 3386 1572 809 ApaI G_GGCC'C Cuts at: 2234 2281 2291 2234 Size: 47 10 5710 Fragments arranged by size: 5710 47 10 ApaLI G'TGCA_C Cuts at: 3824 4980 3824 Size: 1156 4611 ApoI r'AATT_y Cuts at: 1493 3076 3302 3454 5563 1493 Size: 1583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 2274 3092 1870 Size: 73 331 818 4545 Fragments arranged by size: 4545 818 331 73 BamHI G'GATC_C Cuts at: 3097 3464 3097 Size: 367 5400 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2635 3088 4543 618 Size: 325 217 627 848 453 1455 1842 Fragments arranged by size: 1842 1455 848 627 453 325 217 BanII G_rGCy'C Cuts at: 1391 2234 2281 2291 2455 2544 2778 3086 1391 Size: 843 47 10 164 89 234 308 4072 Fragments arranged by size: 4072 843 308 234 164 89 47 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2172 3050 928 Size: 971 273 878 3645 Fragments arranged by size: 3645 971 878 273 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3958 3992 1903 Size: 34 2021 34 3678 Fragments arranged by size: 3678 2021 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2960 3659 4869 2960 Size: 699 1210 3858 Fragments arranged by size: 3858 1210 699 BclI T'GATC_A Cuts at: 1548 1548 Size: 5767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3059 4378 136 Size: 107 122 71 631 605 1387 1319 1525 Fragments arranged by size: 1525 1387 1319 631 605 122 107 71 BglII A'GATC_T Cuts at: 5762 5762 Size: 5767 BlpI GC'TnA_GC Cuts at: 3184 3184 Size: 5767 Bme1580I G_kGCm'C Cuts at: 1790 2214 2234 2281 2291 3828 4984 1790 Size: 424 20 47 10 1537 1156 2573 Fragments arranged by size: 2573 1537 1156 424 47 20 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3498 4456 442 Size: 416 53 83 399 24 2081 958 1753 Fragments arranged by size: 2081 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2220 2966 4427 1239 Size: 981 746 1461 2579 Fragments arranged by size: 2579 1461 981 746 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2485 3892 4757 5055 2485 Size: 1407 865 298 3197 Fragments arranged by size: 3197 1407 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4430 882 Size: 617 2931 2219 Fragments arranged by size: 2931 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3558 493 Size: 711 2354 2702 Fragments arranged by size: 2702 2354 711 BsaBI GATnn'nnATC Cuts at: 5761 5761 Size: 5767 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2589 3574 3956 4591 Size: 53 83 186 936 1056 985 382 635 Cuts at: 4591 275 Size: 1451 Fragments arranged by size: 1451 1056 985 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2568 4200 4598 4872 5019 5430 2568 Size: 1632 398 274 147 411 2905 Fragments arranged by size: 2905 1632 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5542 Fragments arranged by size: 5542 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2482 1186 Size: 1296 4471 BseYI C'CCAG_C Cuts at: 2284 2290 2454 4970 5466 2284 Size: 6 164 2516 496 2585 Fragments arranged by size: 2585 2516 496 164 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2580 1163 Size: 573 844 4350 Fragments arranged by size: 4350 844 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3217 3978 5006 1540 Size: 275 186 1216 761 1028 2301 Fragments arranged by size: 2301 1216 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3828 3913 4984 3828 Size: 85 1071 4611 Fragments arranged by size: 4611 1071 85 BsmI GAATG_Cn' Cuts at: 2259 2824 3278 3371 2259 Size: 565 454 93 4655 Fragments arranged by size: 4655 565 454 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2234 2281 2291 2455 2544 2778 Size: 399 424 20 47 10 164 89 234 Cuts at: 2778 3086 3828 3913 4984 1391 Size: 308 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 424 399 308 234 164 89 85 47 20 10 BspEI T'CCGG_A Cuts at: 5430 5430 Size: 5767 BspHI T'CATG_A Cuts at: 3656 5386 3656 Size: 1730 4037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2718 3122 844 Size: 1874 404 3489 Fragments arranged by size: 3489 1874 404 BsrBI CCG'CTC Cuts at: 1394 1823 3654 1394 Size: 429 1831 3507 Fragments arranged by size: 3507 1831 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2223 4262 4436 65 Size: 1183 975 2039 174 1396 Fragments arranged by size: 2039 1396 1183 975 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2096 2243 4411 4598 1700 Size: 110 286 147 2168 187 2869 Fragments arranged by size: 2869 2168 286 187 147 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5767 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5767 BssSI C'ACGA_G Cuts at: 3827 4839 3827 Size: 1012 4755 BstAPI GCAn_nnn'nTGC Cuts at: 794 2265 794 Size: 1471 4296 BstBI TT'CG_AA Cuts at: 4571 4571 Size: 5767 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5767 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5767 BstYI r'GATC_y Cuts at: 1972 2595 3097 3464 3849 3866 5318 5404 5416 Size: 623 502 367 385 17 1452 86 12 Cuts at: 5416 5762 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 623 502 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2114 2475 513 Size: 552 939 110 361 3805 Fragments arranged by size: 3805 939 552 361 110 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5028 Fragments arranged by size: 5028 683 56 BtsI GCAGTG_nn' Cuts at: 2783 3283 4077 4097 2783 Size: 500 794 20 4453 Fragments arranged by size: 4453 794 500 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5732 DraI TTT'AAA Cuts at: 3071 3071 Size: 5767 DraIII CAC_nnn'GTG Cuts at: 2815 2815 Size: 5767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4774 809 Size: 507 3458 1802 Fragments arranged by size: 3458 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 3060 3214 Size: 54 1425 50 275 18 268 962 154 Cuts at: 3214 4103 5732 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 962 889 275 268 154 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3214 1537 Size: 275 1402 4090 Fragments arranged by size: 4090 1402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2397 3697 1353 Size: 1044 1300 3423 Fragments arranged by size: 3423 1300 1044 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4354 4728 4874 387 Size: 1064 138 2765 374 146 1280 Fragments arranged by size: 2765 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2220 2966 3830 4427 5214 5482 Size: 393 427 161 746 864 597 787 268 Cuts at: 5482 1239 Size: 1524 Fragments arranged by size: 1524 864 787 746 597 427 393 268 161 EcoO109I rG'GnC_Cy Cuts at: 2083 2231 2278 2083 Size: 148 47 5572 Fragments arranged by size: 5572 148 47 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 EcoRV GAT'ATC Cuts at: 2887 2887 Size: 5767 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2408 2408 Size: 5767 FspI TGC'GCA Cuts at: 4273 4273 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 2097 2702 4914 1678 Size: 419 605 2212 2531 Fragments arranged by size: 2531 2212 605 419 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2397 2429 2836 2868 4408 Size: 32 135 32 825 32 407 32 1540 Cuts at: 4408 4440 4482 4514 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1540 825 407 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3357 669 Size: 988 281 1173 246 3079 Fragments arranged by size: 3079 1173 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5767 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KpnI G_GTAC'C Cuts at: 2639 3092 2639 Size: 453 5314 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 5643 5643 Size: 5767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2292 4881 5065 1305 Size: 987 2589 184 2007 Fragments arranged by size: 2589 2007 987 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3062 10 Size: 54 1425 343 1230 2715 Fragments arranged by size: 2715 1425 1230 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2975 3725 4084 4243 518 Size: 648 843 966 750 359 159 2042 Fragments arranged by size: 2042 966 843 750 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2556 3183 3860 5008 5253 1606 Size: 950 627 677 1148 245 2120 Fragments arranged by size: 2120 1148 950 677 627 245 NaeI GCC'GGC Cuts at: 2098 2098 Size: 5767 NcoI C'CATG_G Cuts at: 513 1065 2004 2475 513 Size: 552 939 471 3805 Fragments arranged by size: 3805 939 552 471 NdeI CA'TA_TG Cuts at: 387 387 Size: 5767 NgoMIV G'CCGG_C Cuts at: 2096 2096 Size: 5767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 3214 3214 Size: 5767 NspI r_CATG'y Cuts at: 1132 1229 3125 4670 1132 Size: 97 1896 1545 2229 Fragments arranged by size: 2229 1896 1545 97 PasI CC'CwG_GG Cuts at: 1348 2680 1348 Size: 1332 4435 PciI A'CATG_T Cuts at: 4666 4666 Size: 5767 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5767 PfoI T'CCnGG_A Cuts at: 2236 2236 Size: 5767 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2741 2773 3836 3868 5349 5381 2741 Size: 32 1063 32 1481 32 3127 Fragments arranged by size: 3127 1481 1063 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 3337 3337 Size: 5767 PspOMI G'GGCC_C Cuts at: 2230 2277 2287 2230 Size: 47 10 5710 Fragments arranged by size: 5710 47 10 PstI C_TGCA'G Cuts at: 830 2715 3119 830 Size: 1885 404 3478 Fragments arranged by size: 3478 1885 404 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SfcI C'TryA_G Cuts at: 826 1045 2711 3115 4250 4931 5122 826 Size: 219 1666 404 1135 681 191 1471 Fragments arranged by size: 1666 1471 1135 681 404 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3059 3059 Size: 5767 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5767 SmaI CCC'GGG Cuts at: 1872 2276 3094 1872 Size: 404 818 4545 Fragments arranged by size: 4545 818 404 SmlI C'TyrA_G Cuts at: 820 1017 1943 2464 3871 4772 5034 820 Size: 197 926 521 1407 901 262 1553 Fragments arranged by size: 1553 1407 926 901 521 262 197 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5767 SphI G_CATG'C Cuts at: 1132 3125 1132 Size: 1993 3774 SrfI GCCC'GGGC Cuts at: 2276 2276 Size: 5767 SspI AAT'ATT Cuts at: 5 52 3691 5 Size: 47 3639 2081 Fragments arranged by size: 3639 2081 47 StuI AGG'CCT Cuts at: 1517 3021 1517 Size: 1504 4263 StyI C'CwwG_G Cuts at: 513 1065 2004 2475 3206 513 Size: 552 939 471 731 3074 Fragments arranged by size: 3074 939 731 552 471 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2459 2600 2796 3794 3811 3964 4149 Size: 1255 879 141 196 998 17 153 185 Cuts at: 4149 4594 325 Size: 445 1498 Fragments arranged by size: 1498 1255 998 879 445 196 185 153 141 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4013 5725 96 Size: 274 80 33 51 1443 2036 1712 138 Fragments arranged by size: 2036 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5727 5759 5727 Size: 32 5735 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5767 XbaI T'CTAG_A Cuts at: 3103 3103 Size: 5767 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5767 XmaI C'CCGG_G Cuts at: 1870 2274 3092 1870 Size: 404 818 4545 Fragments arranged by size: 4545 818 404 XmnI GAAnn'nnTTC Cuts at: 2756 3896 2756 Size: 1140 4627 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3575 276 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SwaI XcmI