(Circular) (Six-base) MAPSORT of: pFN21AB6590.seq Check: 2724 from: 1 to: 5923 pFN21AB6590 5923 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2673 3733 278 Size: 53 83 186 936 1137 1060 2468 Fragments arranged by size: 2468 1137 1060 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2877 3266 1937 Size: 940 389 4594 Fragments arranged by size: 4594 940 389 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2162 3986 5370 5638 1632 Size: 530 1824 1384 268 1917 Fragments arranged by size: 1917 1824 1384 530 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 2882 4754 2882 Size: 1872 4051 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2427 2459 2427 Size: 32 5891 AlwNI CAG_nnn'CTG Cuts at: 3666 5238 3666 Size: 1572 4351 ApaI G_GGCC'C Cuts at: 2990 3145 2990 Size: 155 5768 ApaLI G'TGCA_C Cuts at: 3088 3980 5136 3088 Size: 892 1156 3875 Fragments arranged by size: 3875 1156 892 ApoI r'AATT_y Cuts at: 1493 2704 2767 3232 3458 3610 5719 1493 Size: 1211 63 465 226 152 2109 1697 Fragments arranged by size: 2109 1697 1211 465 226 152 63 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2177 3248 1870 Size: 73 234 1071 4545 Fragments arranged by size: 4545 1071 234 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 2338 3244 4699 618 Size: 325 217 627 495 56 906 1455 1842 Fragments arranged by size: 1842 1455 906 627 495 325 217 56 BanII G_rGCy'C Cuts at: 1391 2060 2990 3145 3242 1391 Size: 669 930 155 97 4072 Fragments arranged by size: 4072 930 669 155 97 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2665 2862 928 Size: 971 766 197 3989 Fragments arranged by size: 3989 971 766 197 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3190 3224 4114 4148 1903 Size: 34 1253 34 890 34 3678 Fragments arranged by size: 3678 1253 890 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3149 4534 136 Size: 107 122 71 631 605 1477 1385 1525 Fragments arranged by size: 1525 1477 1385 631 605 122 107 71 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 2343 2990 3092 3145 3984 5140 1790 Size: 553 647 102 53 839 1156 2573 Fragments arranged by size: 2573 1156 839 647 553 102 53 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2251 3654 4612 Size: 416 53 83 399 24 834 1403 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1753 1403 958 834 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2395 4583 1239 Size: 1156 2188 2579 Fragments arranged by size: 2579 2188 1156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2096 4048 4913 5211 2096 Size: 1952 865 298 2808 Fragments arranged by size: 2808 1952 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2078 4586 882 Size: 617 579 2508 2219 Fragments arranged by size: 2508 2219 617 579 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 2276 5917 2276 Size: 3641 2282 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2670 3730 4112 4747 Size: 53 83 186 936 1137 1060 382 635 Cuts at: 4747 275 Size: 1451 Fragments arranged by size: 1451 1137 1060 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2882 4356 4754 5028 5175 5586 2882 Size: 1474 398 274 147 411 3219 Fragments arranged by size: 3219 1474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2207 2237 2427 2457 2630 Size: 30 165 30 804 30 190 30 173 Cuts at: 2630 2660 1178 Size: 30 4441 Fragments arranged by size: 4441 804 190 173 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2215 2335 1186 Size: 1029 120 4774 Fragments arranged by size: 4774 1029 120 BseYI C'CCAG_C Cuts at: 2031 2716 2734 2751 5126 5622 2031 Size: 685 18 17 2375 496 2332 Fragments arranged by size: 2375 2332 685 496 18 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2374 1163 Size: 573 638 4712 Fragments arranged by size: 4712 638 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3092 3984 4069 5140 3092 Size: 892 85 1071 3875 Fragments arranged by size: 3875 1071 892 85 BsmI GAATG_Cn' Cuts at: 2124 3434 3527 2124 Size: 1310 93 4520 Fragments arranged by size: 4520 1310 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2366 2366 Size: 5923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2343 2990 3092 3145 3242 3984 Size: 399 270 283 647 102 53 97 742 Cuts at: 3984 4069 5140 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 647 399 283 270 102 97 85 53 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3278 844 Size: 2434 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2882 4567 4754 1700 Size: 110 1072 1685 187 2869 Fragments arranged by size: 2869 1685 1072 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 2620 1184 Size: 1436 4487 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2264 2248 Size: 16 5907 BstYI r'GATC_y Cuts at: 1972 2103 3253 3620 4005 4022 5474 5560 5572 Size: 131 1150 367 385 17 1452 86 12 Cuts at: 5572 5918 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1150 385 367 346 131 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2496 3108 513 Size: 552 939 492 612 3328 Fragments arranged by size: 3328 939 612 552 492 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 2367 3439 4233 4253 2367 Size: 1072 794 20 4037 Fragments arranged by size: 4037 1072 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 2098 3227 2098 Size: 1129 4794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 2565 3096 Size: 54 1425 50 275 18 333 402 531 Cuts at: 3096 3370 4259 5888 8 Size: 274 889 1629 43 Fragments arranged by size: 1629 1425 889 531 402 333 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2192 2396 2945 3853 1353 Size: 839 204 549 908 3423 Fragments arranged by size: 3423 908 839 549 204 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2395 3986 4583 5370 5638 1239 Size: 393 530 233 1591 597 787 268 1524 Fragments arranged by size: 1591 1524 787 597 530 393 268 233 EcoO109I rG'GnC_Cy Cuts at: 2986 2987 3142 2986 Size: 1 155 5767 Fragments arranged by size: 5767 155 1 EcoRI G'AATT_C Cuts at: 2704 3232 3610 2704 Size: 528 378 5017 Fragments arranged by size: 5017 528 378 EcoRV GAT'ATC Cuts at: 3115 3115 Size: 5923 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 5070 1678 Size: 3392 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2660 2692 4564 4596 4638 Size: 32 135 32 1088 32 1872 32 42 Cuts at: 4638 4670 1373 Size: 32 2626 Fragments arranged by size: 2626 1872 1088 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2165 3098 10 Size: 54 1425 343 333 933 2835 Fragments arranged by size: 2835 1425 933 343 333 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 2540 3881 4240 4399 518 Size: 648 1329 45 1341 359 159 2042 Fragments arranged by size: 2042 1341 1329 648 359 159 45 MspA1I CmG'CkG Cuts at: 1606 3339 4016 5164 5409 1606 Size: 1733 677 1148 245 2120 Fragments arranged by size: 2120 1733 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3108 513 Size: 552 939 1104 3328 Fragments arranged by size: 3328 1104 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 3281 4826 1132 Size: 97 2052 1545 2229 Fragments arranged by size: 2229 2052 1545 97 PasI CC'CwG_GG Cuts at: 1348 3145 1348 Size: 1797 4126 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2334 2716 3109 3128 1108 Size: 1226 382 393 19 3903 Fragments arranged by size: 3903 1226 393 382 19 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2427 2459 3992 4024 5505 5537 2427 Size: 32 1533 32 1481 32 2813 Fragments arranged by size: 2813 1533 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2798 1613 Size: 1185 4738 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspOMI G'GGCC_C Cuts at: 2986 3141 2986 Size: 155 5768 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SapI GCTCTTCn'nnn_ Cuts at: 2396 2396 Size: 5923 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 2261 4171 5883 2261 Size: 1910 1712 2301 Fragments arranged by size: 2301 1910 1712 SfcI C'TryA_G Cuts at: 826 1045 3070 3271 4406 5087 5278 826 Size: 219 2025 201 1135 681 191 1471 Fragments arranged by size: 2025 1471 1135 681 219 201 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3149 3149 Size: 5923 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 2882 1700 Size: 1182 4741 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2111 4027 4928 5190 820 Size: 197 926 168 1916 901 262 1553 Fragments arranged by size: 1916 1553 926 901 262 197 168 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 2936 1517 Size: 1419 4504 StyI C'CwwG_G Cuts at: 513 1065 2004 2650 2937 3108 3213 3362 513 Size: 552 939 646 287 171 105 149 3074 Fragments arranged by size: 3074 939 646 552 287 171 149 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 2326 2489 3139 3950 3967 4120 Size: 1255 690 56 163 650 811 17 153 Cuts at: 4120 4305 4750 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 811 690 650 445 185 163 153 56 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 4169 5881 Size: 274 80 33 51 1443 282 1910 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 1910 1712 1443 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2452 2484 2496 2528 2715 2747 3084 3116 5883 Size: 32 12 32 187 32 337 32 2767 Cuts at: 5883 5915 2452 Size: 32 2460 Fragments arranged by size: 2767 2460 337 187 32 32 32 32 32 12 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3106 3142 3106 Size: 36 5887 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2671 3731 276 Size: 53 83 186 936 1137 1060 2468 Fragments arranged by size: 2468 1137 1060 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI