(Circular) (Six-base) MAPSORT of: pFN21AB5988.seq Check: 2532 from: 1 to: 6379 pFN21AB5988 6379 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4189 278 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2665 2919 3559 3722 1937 Size: 728 254 640 163 4594 Fragments arranged by size: 4594 728 640 254 163 Acc65I G'GTAC_C Cuts at: 3700 3700 Size: 6379 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 2141 2553 4442 5826 6094 1632 Size: 425 84 412 1889 1384 268 1917 Fragments arranged by size: 1917 1889 1384 425 412 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6182 AflIII A'CryG_T Cuts at: 1203 2962 3364 5278 6255 1203 Size: 1759 402 1914 977 1327 Fragments arranged by size: 1914 1759 1327 977 402 AgeI A'CCGG_T Cuts at: 5210 5210 Size: 6379 AhdI GACnn_n'nnGTC Cuts at: 5108 5108 Size: 6379 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6379 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6379 AlwNI CAG_nnn'CTG Cuts at: 3164 4122 5694 3164 Size: 958 1572 3849 Fragments arranged by size: 3849 1572 958 ApaLI G'TGCA_C Cuts at: 4436 5592 4436 Size: 1156 5223 ApoI r'AATT_y Cuts at: 1493 2250 2881 3083 3688 3914 4066 6175 1493 Size: 757 631 202 605 226 152 2109 1697 Fragments arranged by size: 2109 1697 757 631 605 226 202 152 AseI AT'TA_AT Cuts at: 160 1032 3404 4933 160 Size: 872 2372 1529 1606 Fragments arranged by size: 2372 1606 1529 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 3704 1870 Size: 73 1761 4545 Fragments arranged by size: 4545 1761 73 BamHI G'GATC_C Cuts at: 2829 3709 4076 2829 Size: 880 367 5132 Fragments arranged by size: 5132 880 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3700 5155 618 Size: 325 217 627 1913 1455 1842 Fragments arranged by size: 1913 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2341 2724 3698 1391 Size: 950 383 974 4072 Fragments arranged by size: 4072 974 950 383 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2181 928 Size: 971 282 5126 Fragments arranged by size: 5126 971 282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4570 4604 1903 Size: 34 2633 34 3678 Fragments arranged by size: 3678 2633 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3610 4271 5481 3610 Size: 661 1210 4508 Fragments arranged by size: 4508 1210 661 BclI T'GATC_A Cuts at: 1548 1548 Size: 6379 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3641 4990 136 Size: 107 122 71 631 605 1969 1349 1525 Fragments arranged by size: 1969 1525 1349 631 605 122 107 71 BglII A'GATC_T Cuts at: 2737 3439 6374 2737 Size: 702 2935 2742 Fragments arranged by size: 2935 2742 702 BlpI GC'TnA_GC Cuts at: 2379 3796 2379 Size: 1417 4962 Bme1580I G_kGCm'C Cuts at: 1790 3010 3428 4440 5596 1790 Size: 1220 418 1012 1156 2573 Fragments arranged by size: 2573 1220 1156 1012 418 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2432 4110 5068 Size: 416 53 83 399 24 1015 1678 958 Cuts at: 5068 442 Size: 1753 Fragments arranged by size: 1753 1678 1015 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3485 1056 Size: 2429 3950 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3516 3516 Size: 6379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2883 5039 1239 Size: 1644 2156 2579 Fragments arranged by size: 2579 2156 1644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2513 4504 5369 5667 2513 Size: 1991 865 298 3225 Fragments arranged by size: 3225 1991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5042 882 Size: 617 3543 2219 Fragments arranged by size: 3543 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4170 493 Size: 711 2966 2702 Fragments arranged by size: 2966 2702 711 BsaBI GATnn'nnATC Cuts at: 6373 6373 Size: 6379 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4186 4568 5203 275 Size: 53 83 186 936 2653 382 635 1451 Fragments arranged by size: 2653 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4812 5210 5484 5631 6042 4812 Size: 398 274 147 411 5149 Fragments arranged by size: 5149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6154 Fragments arranged by size: 6154 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6379 BseYI C'CCAG_C Cuts at: 3059 3636 5582 6078 3059 Size: 577 1946 496 3360 Fragments arranged by size: 3360 1946 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2309 3338 1163 Size: 573 573 1029 4204 Fragments arranged by size: 4204 1029 573 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3829 4590 5618 1540 Size: 275 186 1828 761 1028 2301 Fragments arranged by size: 2301 1828 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2724 3502 3541 4440 4525 5596 2724 Size: 778 39 899 85 1071 3507 Fragments arranged by size: 3507 1071 899 778 85 39 BsmI GAATG_Cn' Cuts at: 3890 3983 3890 Size: 93 6286 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2341 2724 3010 3428 3502 3541 3698 Size: 399 551 383 286 418 74 39 157 Cuts at: 3698 4440 4525 5596 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 551 418 399 383 286 157 85 74 39 BspEI T'CCGG_A Cuts at: 6042 6042 Size: 6379 BspHI T'CATG_A Cuts at: 3616 4268 5998 3616 Size: 652 1730 3997 Fragments arranged by size: 3997 1730 652 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3734 844 Size: 2890 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4266 1394 Size: 429 2443 3507 Fragments arranged by size: 3507 2443 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2526 4874 5048 65 Size: 1183 1278 2348 174 1396 Fragments arranged by size: 2348 1396 1278 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5023 5210 1700 Size: 110 3213 187 2869 Fragments arranged by size: 3213 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3460 96 Size: 3364 3015 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 4439 5451 4439 Size: 1012 5367 BstAPI GCAn_nnn'nTGC Cuts at: 794 2433 2885 3005 3164 794 Size: 1639 452 120 159 4009 Fragments arranged by size: 4009 1639 452 159 120 BstBI TT'CG_AA Cuts at: 5183 5183 Size: 6379 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6379 BstXI CCAn_nnnn'nTGG Cuts at: 2147 2147 Size: 6379 BstYI r'GATC_y Cuts at: 1972 2737 2829 3439 3548 3709 4076 4461 4478 Size: 765 92 610 109 161 367 385 17 Cuts at: 4478 5930 6016 6028 6374 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 765 610 385 367 346 161 109 92 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3348 3522 513 Size: 552 939 1344 174 3370 Fragments arranged by size: 3370 1344 939 552 174 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5640 Fragments arranged by size: 5640 683 56 BtsI GCAGTG_nn' Cuts at: 2431 3426 3895 4689 4709 2431 Size: 995 469 794 20 4101 Fragments arranged by size: 4101 995 794 469 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6344 DraI TTT'AAA Cuts at: 3683 3683 Size: 6379 DraIII CAC_nnn'GTG Cuts at: 3005 3005 Size: 6379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2185 5386 809 Size: 507 869 3201 1802 Fragments arranged by size: 3201 1802 869 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3021 3826 4715 Size: 54 1425 50 275 18 1191 805 889 Cuts at: 4715 6344 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1191 889 805 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3826 1537 Size: 275 2014 4090 Fragments arranged by size: 4090 2014 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2529 2555 4309 1353 Size: 1176 26 1754 3423 Fragments arranged by size: 3423 1754 1176 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4966 5340 5486 387 Size: 1064 138 3377 374 146 1280 Fragments arranged by size: 3377 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2722 2722 Size: 6379 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2141 2553 2883 4442 5039 5826 Size: 393 425 84 412 330 1559 597 787 Cuts at: 5826 6094 1239 Size: 268 1524 Fragments arranged by size: 1559 1524 787 597 425 412 393 330 268 84 EcoRI G'AATT_C Cuts at: 2250 3688 4066 2250 Size: 1438 378 4563 Fragments arranged by size: 4563 1438 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2339 2399 2339 Size: 60 6319 FspI TGC'GCA Cuts at: 4885 4885 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 5526 1678 Size: 3848 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3523 3555 3584 3616 5020 Size: 32 135 32 1951 32 29 32 1404 Cuts at: 5020 5052 5094 5126 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1951 1404 135 42 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 2053 2178 3723 3969 669 Size: 988 281 115 125 1545 246 3079 Fragments arranged by size: 3079 1545 988 281 246 125 115 HindIII A'AGCT_T Cuts at: 748 748 Size: 6379 HpaI GTT'AAC Cuts at: 3969 3969 Size: 6379 KpnI G_GTAC'C Cuts at: 3704 3704 Size: 6379 MfeI C'AATT_G Cuts at: 2874 3173 3978 2874 Size: 299 805 5275 Fragments arranged by size: 5275 805 299 MluI A'CGCG_T Cuts at: 6255 6255 Size: 6379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5493 5677 1305 Size: 4188 184 2007 Fragments arranged by size: 4188 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3023 10 Size: 54 1425 343 1191 3366 Fragments arranged by size: 3366 1425 1191 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3237 4337 4696 4855 518 Size: 648 2071 1100 359 159 2042 Fragments arranged by size: 2071 2042 1100 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 2161 2293 2638 3059 3795 4472 5620 Size: 442 113 132 345 421 736 677 1148 Cuts at: 5620 5865 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 736 677 442 421 345 245 132 113 NcoI C'CATG_G Cuts at: 513 1065 2004 3348 3522 513 Size: 552 939 1344 174 3370 Fragments arranged by size: 3370 1344 939 552 174 NdeI CA'TA_TG Cuts at: 387 2351 2913 387 Size: 1964 562 3853 Fragments arranged by size: 3853 1964 562 NheI G'CTAG_C Cuts at: 1052 3481 1052 Size: 2429 3950 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NspI r_CATG'y Cuts at: 1132 1229 2966 2996 3368 3737 5282 1132 Size: 97 1737 30 372 369 1545 2229 Fragments arranged by size: 2229 1737 1545 372 369 97 30 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6379 PciI A'CATG_T Cuts at: 2962 3364 5278 2962 Size: 402 1914 4063 Fragments arranged by size: 4063 1914 402 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6379 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4448 4480 5961 5993 4448 Size: 32 1481 32 4834 Fragments arranged by size: 4834 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3265 1613 Size: 1652 4727 PsiI TTA'TAA Cuts at: 3035 3949 3035 Size: 914 5465 PstI C_TGCA'G Cuts at: 830 3731 830 Size: 2901 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6379 PvuII CAG'CTG Cuts at: 2048 2161 2293 2638 3059 2048 Size: 113 132 345 421 5368 Fragments arranged by size: 5368 421 345 132 113 SacI G_AGCT'C Cuts at: 2724 2724 Size: 6379 SalI G'TCGA_C Cuts at: 1936 3721 1936 Size: 1785 4594 SapI GCTCTTCn'nnn_ Cuts at: 2529 2529 Size: 6379 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 4627 6339 4627 Size: 1712 4667 SfcI C'TryA_G Cuts at: 826 1045 2633 2852 3727 4862 5543 5734 826 Size: 219 1588 219 875 1135 681 191 1471 Fragments arranged by size: 1588 1471 1135 875 681 219 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 3706 1872 Size: 1834 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2492 4483 5384 5646 820 Size: 197 926 549 1991 901 262 1553 Fragments arranged by size: 1991 1553 926 901 549 262 197 SnaBI TAC'GTA Cuts at: 493 4170 493 Size: 3677 2702 SpeI A'CTAG_T Cuts at: 152 3356 152 Size: 3204 3175 SphI G_CATG'C Cuts at: 1132 2996 3737 1132 Size: 1864 741 3774 Fragments arranged by size: 3774 1864 741 SspI AAT'ATT Cuts at: 5 52 4303 5 Size: 47 4251 2081 Fragments arranged by size: 4251 2081 47 StuI AGG'CCT Cuts at: 1517 2652 3644 1517 Size: 1135 992 4252 Fragments arranged by size: 4252 1135 992 StyI C'CwwG_G Cuts at: 513 1065 2004 3348 3522 3818 513 Size: 552 939 1344 174 296 3074 Fragments arranged by size: 3074 1344 939 552 296 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2384 4406 4423 4576 4761 5206 325 Size: 1255 804 2022 17 153 185 445 1498 Fragments arranged by size: 2022 1498 1255 804 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2519 2986 3460 Size: 274 80 33 51 1443 542 467 474 Cuts at: 3460 3571 4625 6337 96 Size: 111 1054 1712 138 Fragments arranged by size: 1712 1443 1054 542 474 467 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6339 6371 6339 Size: 32 6347 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6379 XbaI T'CTAG_A Cuts at: 3442 3589 3715 3442 Size: 147 126 6106 Fragments arranged by size: 6106 147 126 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6379 XmaI C'CCGG_G Cuts at: 1870 3704 1870 Size: 1834 4545 XmnI GAAnn'nnTTC Cuts at: 4508 4508 Size: 6379 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4187 276 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI