Restriction map for
pFN21AB7355
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# NO CUT ENZYMES ( 108 enzymes) #
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Acc65I AccII AccIII AciI AclI
AflII AgeI AhdI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcnI BfrI
BglI BlnI BsaI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BstAPI BstBI BstEII
BstPI BstUI Cfr10I ClaI CpoI
DraIII DsaI EaeI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI HindIII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NsiI NspV PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1469 GACGTC GACGT^C
AccI 1486 GTATAC GT^[AC][GT]AC
Alw44I 1335 GTGCAC G^TGCAC
AocI 949 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2007 AGCGCT AGC^GCT
ApaLI 1335 GTGCAC G^TGCAC
Asp700I 3039 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3218 TGCGCA TGC^GCA
BanII 3019 GGGCTC G[GA]GC[CT]^C
BcgI 1392 CGACCAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1686 TGATCA T^GATCA
BlpI 175 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 175 GCTCAGC GC^T[ACGT]AGC
BsaAI 274 TACGTG [CT]AC^GT[GA]
BsaBI 246 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 2787 CTCCTC GAGGAG(10/8)
BsmBI 1765 GAGACG CGTCTC(1/5)
BsmI 2259 GAATGC GAATGC(1/-1)
Bsp1407I 2293 TGTACA T^GTACA
BspMI 648 GCAGGT ACCTGC(4/8)
BsrDI 330 CATTGC GCAATG(2/0)
BsrGI 2293 TGTACA T^GTACA
BssSI 1926 CACGAG CACGAG(-5/-1)
Bst1107I 1486 GTATAC GTA^TAC
BstXI 742 CCAACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1486 GTATAC GTA^TAC
Bsu36I 949 CCTCAGG CC^T[ACGT]AGG
CelII 175 GCTCAGC GC^T[ACGT]AGC
Eco47III 2007 AGCGCT AGC^GCT
Eco81I 949 CCTCAGG CC^T[ACGT]AGG
EcoRI 2378 GAATTC G^AATTC
FbaI 1686 TGATCA T^GATCA
FspI 3218 TGCGCA TGC^GCA
HgaI 2546 GCGTC GACGC(5/10)
HincII 140 GTCAAC GT[CT]^[GA]AC
HindII 140 GTCAAC GT[CT]^[GA]AC
MamI 246 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 570 GCTAGC G^CTAGC
PacI 916 TTAATTAA TTAAT^TAA
SapI 1372 GCTCTTC GCTCTTC(1/4)
SexAI 485 ACCTGGT A^CC[AT]GGT
SphI 574 GCATGC GCATG^C
SspBI 2293 TGTACA T^GTACA
SspI 2106 AATATT AAT^ATT
XcmI 3005 CCAGGCCTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3039 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1469 GACGTC G[GA]^CG[CT]C
2545 GGCGTC
AspHI 1335 GTGCAC G[AT]GC[AT]^C
1370 GTGCTC
BglII 2031 AGATCT A^GATCT
2166 AGATCT
BsaHI 1469 GACGTC G[GA]^CG[CT]C
2545 GGCGTC
BsaWI 1603 ACCGGA [AT]^CCGG[AT]
2642 ACCGGA
BsiHKAI 1335 GTGCAC G[AT]GC[AT]^C
1370 GTGCTC
DraII 871 AGGTCCT [GA]G^G[ACGT]CC[CT]
3098 AGGTCCT
EcoO109I 871 AGGTCCT [GA]G^G[ACGT]CC[CT]
3098 AGGTCCT
EcoT14I 906 CCTTGG C^C[AT][AT]GG
2439 CCTTGG
HaeII 3 GGCGCT [GA]GCGC^[CT]
2007 AGCGCT
HaeIII 1230 GGCC GG^CC
3008 GGCC
HapII 1604 CCGG C^CGG
2643 CCGG
Hin1I 1469 GACGTC G[GA]^CG[CT]C
2545 GGCGTC
HpaII 1604 CCGG C^CGG
2643 CCGG
MspA1I 1021 CAGCTG C[AC]G^C[GT]G
2793 CAGCTG
MspI 1604 CCGG C^CGG
2643 CCGG
NdeI 1676 CATATG CA^TATG
2695 CATATG
PflMI 15 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1429 CCATATTCTGG
PpuMI 871 AGGTCCT [GA]G^G[AT]CC[CT]
3098 AGGTCCT
PstI 713 CTGCAG CTGCA^G
2935 CTGCAG
PvuII 1021 CAGCTG CAG^CTG
2793 CAGCTG
SmlI 2479 CTCAAG C^T[CT][GA]AG
2509 CTCAAG
StuI 1229 AGGCCT AGG^CCT
3007 AGGCCT
StyI 906 CCTTGG C^C[AT][AT]GG
2439 CCTTGG
TfiI 709 GAATC G^A[AT]TC
738 GATTC
Van91I 15 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1429 CCATATTCTGG
XbaI 499 TCTAGA T^CTAGA
1761 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI AvaII BbsI BbvI
BciVI BfaI BmyI BpmI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspLU11I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DrdI EarI EcoRII Fnu4HI FokI
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII