(Circular) (Six-base) MAPSORT of: pFN21AB7355.seq Check: 3186 from: 1 to: 7969 pFN21AB7355 7969 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 5779 278 Size: 53 83 186 936 1942 2301 2468 Fragments arranged by size: 2468 2301 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3492 5312 1937 Size: 1555 1820 4594 Fragments arranged by size: 4594 1820 1555 Acc65I G'GTAC_C Cuts at: 5290 5290 Size: 7969 AclI AA'CG_TT Cuts at: 1563 6096 6469 1563 Size: 4533 373 3063 Fragments arranged by size: 4533 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2374 2496 2606 3081 3827 3970 4244 4378 Size: 742 122 110 475 746 143 274 134 Cuts at: 4378 5104 6032 7416 7684 1632 Size: 726 928 1384 268 1917 Fragments arranged by size: 1917 1384 928 746 742 726 475 274 268 143 134 122 110 AfeI AGC'GCT Cuts at: 4014 4014 Size: 7969 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7772 AflIII A'CryG_T Cuts at: 1203 2198 3371 4295 6868 7845 1203 Size: 995 1173 924 2573 977 1327 Fragments arranged by size: 2573 1327 1173 995 977 924 AgeI A'CCGG_T Cuts at: 6800 6800 Size: 7969 AhdI GACnn_n'nnGTC Cuts at: 6698 6698 Size: 7969 AleI CACnn'nnGTG Cuts at: 1166 3933 1166 Size: 2767 5202 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7969 AlwNI CAG_nnn'CTG Cuts at: 4438 4803 5152 5712 7284 4438 Size: 365 349 560 1572 5123 Fragments arranged by size: 5123 1572 560 365 349 ApaLI G'TGCA_C Cuts at: 3340 6026 7182 3340 Size: 2686 1156 4127 Fragments arranged by size: 4127 2686 1156 ApoI r'AATT_y Cuts at: 1493 2987 3113 3911 4058 4066 4357 4383 5278 Size: 1494 126 798 147 8 291 26 895 Cuts at: 5278 5504 5656 7765 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1494 895 798 291 226 152 147 126 26 8 AseI AT'TA_AT Cuts at: 160 1032 6523 160 Size: 872 5491 1606 Fragments arranged by size: 5491 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7969 AvaI C'yCGr_G Cuts at: 1870 1943 5294 1870 Size: 73 3351 4545 Fragments arranged by size: 4545 3351 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4773 4806 4773 Size: 33 7936 BamHI G'GATC_C Cuts at: 5299 5666 5299 Size: 367 7602 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5290 6745 618 Size: 325 217 627 3503 1455 1842 Fragments arranged by size: 3503 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 5028 5288 1391 Size: 3637 260 4072 Fragments arranged by size: 4072 3637 260 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2380 4546 4989 928 Size: 971 481 2166 443 3908 Fragments arranged by size: 3908 2166 971 481 443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3386 3420 6160 6194 1903 Size: 34 1449 34 2740 34 3678 Fragments arranged by size: 3678 2740 1449 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2934 3598 5017 5861 7071 2934 Size: 664 1419 844 1210 3832 Fragments arranged by size: 3832 1419 1210 844 664 BclI T'GATC_A Cuts at: 1548 3691 1548 Size: 2143 5826 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6580 136 Size: 107 122 71 631 605 4908 1525 Fragments arranged by size: 4908 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4036 4171 7964 4036 Size: 135 3793 4041 Fragments arranged by size: 4041 3793 135 BlpI GC'TnA_GC Cuts at: 2181 5386 2181 Size: 3205 4764 Bme1580I G_kGCm'C Cuts at: 1790 3344 6030 7186 1790 Size: 1554 2686 1156 2573 Fragments arranged by size: 2686 2573 1554 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7969 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3118 3817 5107 Size: 416 53 83 399 24 1701 699 1290 Cuts at: 5107 5700 6658 442 Size: 593 958 1753 Fragments arranged by size: 1753 1701 1290 958 699 593 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2579 1056 Size: 1523 6446 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 4577 4780 6629 1239 Size: 1169 2169 203 1849 2579 Fragments arranged by size: 2579 2169 1849 1169 203 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4469 4499 6094 6959 7257 4469 Size: 30 1595 865 298 5181 Fragments arranged by size: 5181 1595 865 298 30 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6632 882 Size: 617 5133 2219 Fragments arranged by size: 5133 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2281 5760 493 Size: 711 1077 3479 2702 Fragments arranged by size: 3479 2702 1077 711 BsaBI GATnn'nnATC Cuts at: 2255 7963 2255 Size: 5708 2261 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3475 4551 5776 6158 Size: 53 83 186 936 1942 1076 1225 382 Cuts at: 6158 6793 275 Size: 635 1451 Fragments arranged by size: 1942 1451 1225 1076 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3608 4647 6402 6800 7074 7221 7632 3608 Size: 1039 1755 398 274 147 411 3945 Fragments arranged by size: 3945 1755 1039 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3024 3054 3323 3353 1178 Size: 30 165 30 1621 30 269 30 5794 Fragments arranged by size: 5794 1621 269 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4783 1186 Size: 3597 4372 BseYI C'CCAG_C Cuts at: 3028 4347 7172 7668 3028 Size: 1319 2825 496 3329 Fragments arranged by size: 3329 2825 1319 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1997 2759 3407 1163 Size: 573 261 762 648 5725 Fragments arranged by size: 5725 762 648 573 261 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5419 6180 7208 1540 Size: 275 186 3418 761 1028 2301 Fragments arranged by size: 3418 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3344 3379 6030 6115 7186 3344 Size: 35 2651 85 1071 4127 Fragments arranged by size: 4127 2651 1071 85 35 BsmI GAATG_Cn' Cuts at: 4270 5480 5573 4270 Size: 1210 93 6666 Fragments arranged by size: 6666 1210 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3764 3764 Size: 7969 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3344 3379 5028 5288 6030 6115 7186 Size: 399 1554 35 1649 260 742 85 1071 Cuts at: 7186 1391 Size: 2174 Fragments arranged by size: 2174 1649 1554 1071 742 399 260 85 35 BspEI T'CCGG_A Cuts at: 7632 7632 Size: 7969 BspHI T'CATG_A Cuts at: 2983 3777 4962 5858 7588 2983 Size: 794 1185 896 1730 3364 Fragments arranged by size: 3364 1730 1185 896 794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2644 5324 844 Size: 1800 2680 3489 Fragments arranged by size: 3489 2680 1800 BsrBI CCG'CTC Cuts at: 1394 1823 5856 1394 Size: 429 4033 3507 Fragments arranged by size: 4033 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2334 6464 6638 65 Size: 1183 1086 4130 174 1396 Fragments arranged by size: 4130 1396 1183 1086 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6613 6800 1700 Size: 110 4803 187 2869 Fragments arranged by size: 4803 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4298 96 Size: 4202 3767 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7969 BssSI C'ACGA_G Cuts at: 3931 6029 7041 3931 Size: 2098 1012 4859 Fragments arranged by size: 4859 2098 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7969 BstBI TT'CG_AA Cuts at: 6773 6773 Size: 7969 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7969 BstXI CCAn_nnnn'nTGG Cuts at: 2754 2754 Size: 7969 BstYI r'GATC_y Cuts at: 1972 2788 4036 4171 4343 4434 5299 5666 6051 Size: 816 1248 135 172 91 865 367 385 Cuts at: 6051 6068 7520 7606 7618 7964 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1248 865 816 385 367 346 172 135 91 86 17 12 BstZ17I GTA'TAC Cuts at: 3493 3493 Size: 7969 Bsu36I CC'TnA_GG Cuts at: 2955 2955 Size: 7969 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6478 Fragments arranged by size: 6478 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7230 Fragments arranged by size: 7230 683 56 BtsI GCAGTG_nn' Cuts at: 2440 5233 5485 6279 6299 2440 Size: 2793 252 794 20 4110 Fragments arranged by size: 4110 2793 794 252 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7969 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7934 DraI TTT'AAA Cuts at: 2592 2616 3012 3882 4129 5273 2592 Size: 24 396 870 247 1144 5288 Fragments arranged by size: 5288 1144 870 396 247 24 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2384 2875 4823 6976 809 Size: 507 1068 491 1948 2153 1802 Fragments arranged by size: 2153 1948 1802 1068 507 491 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5416 6305 7934 Size: 54 1425 50 275 18 3586 889 1629 Cuts at: 7934 8 Size: 43 Fragments arranged by size: 3586 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5416 1537 Size: 275 3604 4090 Fragments arranged by size: 4090 3604 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3384 3846 4397 4841 5197 5899 1353 Size: 2031 462 551 444 356 702 3423 Fragments arranged by size: 3423 2031 702 551 462 444 356 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6556 6930 7076 387 Size: 1064 138 4967 374 146 1280 Fragments arranged by size: 4967 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2374 2408 2496 2606 3081 3827 3970 Size: 393 742 34 88 110 475 746 143 Cuts at: 3970 4244 4378 4577 4780 5104 6032 6629 7416 Size: 274 134 199 203 324 928 597 787 Cuts at: 7416 7684 1239 Size: 268 1524 Fragments arranged by size: 1524 928 787 746 742 597 475 393 324 274 268 203 199 143 134 110 88 34 EcoO109I rG'GnC_Cy Cuts at: 2877 5104 2877 Size: 2227 5742 EcoRI G'AATT_C Cuts at: 4383 5278 5656 4383 Size: 895 378 6696 Fragments arranged by size: 6696 895 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3494 4540 3494 Size: 1046 6923 FspI TGC'GCA Cuts at: 5225 6475 5225 Size: 1250 6719 HaeII r_GCGC'y Cuts at: 1678 2012 4016 7116 1678 Size: 334 2004 3100 2531 Fragments arranged by size: 3100 2531 2004 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2445 2477 3007 3039 3361 Size: 32 135 32 873 32 530 32 322 Cuts at: 3361 3393 4021 4053 4355 4387 6610 6642 6684 Size: 32 628 32 302 32 2223 32 42 Cuts at: 6684 6716 1373 Size: 32 2626 Fragments arranged by size: 2626 2223 873 628 530 322 302 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2147 5313 5559 669 Size: 988 281 209 3166 246 3079 Fragments arranged by size: 3166 3079 988 281 246 209 HindIII A'AGCT_T Cuts at: 748 748 Size: 7969 HpaI GTT'AAC Cuts at: 5559 5559 Size: 7969 KpnI G_GTAC'C Cuts at: 5294 5294 Size: 7969 MfeI C'AATT_G Cuts at: 5568 5568 Size: 7969 MluI A'CGCG_T Cuts at: 7845 7845 Size: 7969 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2771 3234 3309 4970 7083 7267 1305 Size: 1466 463 75 1661 2113 184 2007 Fragments arranged by size: 2113 2007 1661 1466 463 184 75 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6147 Fragments arranged by size: 6147 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2845 3272 3288 3933 4873 5927 6286 Size: 648 1679 427 16 645 940 1054 359 Cuts at: 6286 6445 518 Size: 159 2042 Fragments arranged by size: 2042 1679 1054 940 648 645 427 359 159 16 MspA1I CmG'CkG Cuts at: 1606 3028 4800 5385 6062 7210 7455 1606 Size: 1422 1772 585 677 1148 245 2120 Fragments arranged by size: 2120 1772 1422 1148 677 585 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6478 Fragments arranged by size: 6478 939 552 NdeI CA'TA_TG Cuts at: 387 3682 4701 387 Size: 3295 1019 3655 Fragments arranged by size: 3655 3295 1019 NheI G'CTAG_C Cuts at: 1052 2575 1052 Size: 1523 6446 NotI GC'GGCC_GC Cuts at: 5416 5416 Size: 7969 NspI r_CATG'y Cuts at: 1132 1229 2202 2583 3375 4299 5327 6872 1132 Size: 97 973 381 792 924 1028 1545 2229 Fragments arranged by size: 2229 1545 1028 973 924 792 381 97 PacI TTA_AT'TAA Cuts at: 2925 2925 Size: 7969 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7969 PciI A'CATG_T Cuts at: 2198 3371 4295 6868 2198 Size: 1173 924 2573 3299 Fragments arranged by size: 3299 2573 1173 924 PflMI CCAn_nnn'nTGG Cuts at: 1108 2026 3440 1108 Size: 918 1414 5637 Fragments arranged by size: 5637 1414 918 PfoI T'CCnGG_A Cuts at: 2769 4677 2769 Size: 1908 6061 PmeI GTTT'AAAC Cuts at: 5273 5273 Size: 7969 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6038 6070 7551 7583 6038 Size: 32 1481 32 6424 Fragments arranged by size: 6424 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2877 5104 2877 Size: 2227 5742 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7969 PsiI TTA'TAA Cuts at: 5539 5539 Size: 7969 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3156 3188 3156 Size: 32 7937 PstI C_TGCA'G Cuts at: 830 2722 4944 5321 830 Size: 1892 2222 377 3478 Fragments arranged by size: 3478 2222 1892 377 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7969 PvuII CAG'CTG Cuts at: 3028 4800 3028 Size: 1772 6197 SalI G'TCGA_C Cuts at: 1936 5311 1936 Size: 3375 4594 SapI GCTCTTCn'nnn_ Cuts at: 3384 3384 Size: 7969 SbfI CC_TGCA'GG Cuts at: 5321 5321 Size: 7969 ScaI AGT'ACT Cuts at: 6217 7929 6217 Size: 1712 6257 SexAI A'CCwGG_T Cuts at: 2490 2490 Size: 7969 SfcI C'TryA_G Cuts at: 826 1045 2718 3101 4940 5317 6452 7133 7324 Size: 219 1673 383 1839 377 1135 681 191 Cuts at: 7324 826 Size: 1471 Fragments arranged by size: 1839 1673 1471 1135 681 383 377 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7969 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7969 SmaI CCC'GGG Cuts at: 1872 5296 1872 Size: 3424 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4484 4514 6073 6974 7236 820 Size: 197 926 2541 30 1559 901 262 1553 Fragments arranged by size: 2541 1559 1553 926 901 262 197 30 SnaBI TAC'GTA Cuts at: 493 5760 493 Size: 5267 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7969 SphI G_CATG'C Cuts at: 1132 2583 5327 1132 Size: 1451 2744 3774 Fragments arranged by size: 3774 2744 1451 SspI AAT'ATT Cuts at: 5 52 4113 5893 5 Size: 47 4061 1780 2081 Fragments arranged by size: 4061 2081 1780 47 StuI AGG'CCT Cuts at: 1517 3236 5014 1517 Size: 1719 1778 4472 Fragments arranged by size: 4472 1778 1719 StyI C'CwwG_G Cuts at: 513 1065 2004 2911 4444 5408 513 Size: 552 939 907 1533 964 3074 Fragments arranged by size: 3074 1533 964 939 907 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4031 5195 5996 6013 6166 6351 6796 Size: 1255 2451 1164 801 17 153 185 445 Cuts at: 6796 325 Size: 1498 Fragments arranged by size: 2451 1498 1255 1164 801 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2048 3536 4298 Size: 274 80 33 51 1443 71 1488 762 Cuts at: 4298 4588 4732 6215 7927 96 Size: 290 144 1483 1712 138 Fragments arranged by size: 1712 1488 1483 1443 762 290 274 144 138 80 71 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3323 3355 7929 7961 3323 Size: 32 4574 32 3331 Fragments arranged by size: 4574 3331 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7969 XbaI T'CTAG_A Cuts at: 2504 3766 5305 2504 Size: 1262 1539 5168 Fragments arranged by size: 5168 1539 1262 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5017 5017 Size: 7969 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7969 XmaI C'CCGG_G Cuts at: 1870 5294 1870 Size: 3424 4545 XmnI GAAnn'nnTTC Cuts at: 5048 6098 5048 Size: 1050 6919 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 5777 276 Size: 53 83 186 936 1942 2301 2468 Fragments arranged by size: 2468 2301 1942 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PmlI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI