Restriction map for
pFN21AB8652
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# NO CUT ENZYMES ( 171 enzymes) #
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Acc65I AccI AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BcgI BciVI
BcnI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HapII HgaI HhaI
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 220 GACGTC GACGT^C
AcsI 448 AAATTC [GA]^AATT[CT]
AcyI 220 GACGTC G[GA]^CG[CT]C
AlwI 508 GGATC GGATC(4/5)
ApoI 448 AAATTC [GA]^AATT[CT]
AvaII 27 GGACC G^G[AT]CC
BalI 413 TGGCCA TGG^CCA
BbvCI 589 GCTGAGG CCTCAGC(-5/-2)
BbvI 408 GCTGC GCAGC(8/12)
BclI 522 TGATCA T^GATCA
BfaI 550 CTAG C^TAG
BglII 290 AGATCT A^GATCT
BmyI 393 GTGCCC G[AGT]GC[ACT]^C
BsaHI 220 GACGTC G[GA]^CG[CT]C
BsaI 574 GAGACC GGTCTC(1/5)
BsmAI 574 GAGAC GTCTC(1/5)
BsmFI 275 GGGAC GGGAC(10/14)
Bsp1286I 393 GTGCCC G[AGT]GC[ACT]^C
BspHI 21 TCATGA T^CATGA
BspMI 340 GCAGGT ACCTGC(4/8)
BssKI 356 CCTGG ^CC[ACGT]GG
BstNI 356 CCTGG CC^[AT]GG
BstYI 290 AGATCT [GA]^GATC[CT]
DraI 145 TTTAAA TTT^AAA
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoRII 356 CCTGG ^CC[AT]GG
FauI 153 GCGGG CCCGC(4/6)
FbaI 522 TGATCA T^GATCA
Hin1I 220 GACGTC G[GA]^CG[CT]C
HinfI 58 GAGTC G^A[ACGT]TC
MaeI 550 CTAG C^TAG
MaeII 221 ACGT A^CGT
MaeIII 218 GTGAC ^GT[ACGT]AC
MflI 290 AGATCT [GA]^GATC[CT]
MluNI 413 TGGCCA TGG^CCA
MscI 413 TGGCCA TGG^CCA
MslI 312 CACGCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 356 CCTGG CC^[AT]GG
NlaIV 250 GGGGCC GG[ACGT]^[ACGT]CC
NotI 4 GCGGCCGC GC^GGCCGC
PleI 58 GAGTC GAGTC(4/5)
RcaI 21 TCATGA T^CATGA
ScrFI 356 CCTGG CC^[ACGT]GG
SfaNI 586 GATGC GCATC(5/9)
TaqI 485 TCGA T^CGA
TseI 408 GCTGC G^C[AT]GC
Tsp45I 218 GTGAC ^GT[GC]AC
TthHB8I 485 TCGA T^CGA
XhoII 290 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BsiEI 5 CGGCCG CG[GA][CT]^CG
348 CGACCG
BsrI 120 ACTGG ACTGG(1/-1)
214 ACTGG
Cac8I 153 GCGGGC GC[ACGT]^[ACGT]GC
411 GCTGGC
Cfr13I 27 GGACC G^G[ACGT]CC
251 GGGCC
EaeI 5 CGGCCG [CT]^GGCC[GA]
413 TGGCCA
EcoT14I 11 CCAAGG C^C[AT][AT]GG
578 CCAAGG
HphI 217 GGTGA GGTGA(8/7)
564 GGTGA
MseI 113 TTAA T^TAA
146 TTAA
Sau96I 27 GGACC G^G[ACGT]CC
251 GGGCC
StyI 11 CCAAGG C^C[AT][AT]GG
578 CCAAGG
Tru9I 113 TTAA T^TAA
146 TTAA
Tsp509I 111 AATT ^AATT
449 AATT
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BsaJI BseRI BsiYI
BslI BsmI BstF5I DdeI DpnII
EarI Fnu4HI FokI HaeIII ItaI
Ksp632I MboI MboII MnlI MwoI
NdeII NlaIII Sau3AI TspRI