Restriction map for
pFN21AE0117
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BclI BcnI BfaI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstF5I BstPI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MaeI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 327 ACACGT A^C[GA][CT]GT
AseI 472 ATTAAT AT^TAAT
AsnI 472 ATTAAT AT^TAAT
Asp700I 483 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 461 CCCGAG C^[CT]CG[GA]G
AvaII 284 GGTCC G^G[AT]CC
BbsI 254 GTCTTC GAAGAC(2/6)
BcgI 393 GCAAGTGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 182 AGATCT A^GATCT
BpmI 178 CTCCAG CTGGAG(16/14)
BpuAI 254 GTCTTC GAAGAC(2/6)
BsaBI 537 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 316 GAGACC GGTCTC(1/5)
BseRI 624 GAGGAG GAGGAG(10/8)
BsoBI 461 CCCGAG C^[CT]CG[GA]G
BspMI 281 GCAGGT ACCTGC(4/8)
BssSI 406 CACGAG CACGAG(-5/-1)
BstAPI 59 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 470 CCATTAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 227 GCGC GCG^C
Cfr13I 284 GGTCC G^G[ACGT]CC
DdeI 243 CTCAG C^T[ACGT]AG
DraII 283 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoNI 230 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 283 AGGTCCT [GA]G^G[ACGT]CC[CT]
HapII 27 CCGG C^CGG
HhaI 227 GCGC GCG^C
HinP1I 227 GCGC G^CGC
HpaII 27 CCGG C^CGG
KspI 194 CCGCGG CCGC^GG
MamI 537 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 194 CCGCGG C[AC]G^C[GT]G
MspI 27 CCGG C^CGG
PleI 399 GACTC GAGTC(4/5)
PpuMI 283 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 472 ATTAAT AT^TAAT
SacII 194 CCGCGG CCGC^GG
Sau96I 284 GGTCC G^G[ACGT]CC
SfcI 234 CTACAG C^T[GA][CT]AG
TaqI 155 TCGA T^CGA
TfiI 46 GATTC G^A[AT]TC
Tsp509I 345 AATT ^AATT
TspRI 53 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 155 TCGA T^CGA
XcmI 116 CCACCGTGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 483 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 195 CGCG CG^CG
226 CGCG
AluI 176 AGCT AG^CT
518 AGCT
AlwI 72 GATCC GGATC(4/5)
215 GATCC
BbvI 424 GCTGC GCAGC(8/12)
427 GCTGC
BciVI 189 GGATAC GTATCC(6/5)
381 GGATAC
BsmAI 316 GAGAC GTCTC(1/5)
506 GAGAC
BsrI 420 ACTGG ACTGG(1/-1)
21 CCAGT
BstUI 195 CGCG CG^CG
226 CGCG
BstYI 182 AGATCT [GA]^GATC[CT]
214 AGATCC
DsaI 119 CCGTGG C^C[GA][CT]GG
194 CCGCGG
FauI 140 CCCGC CCCGC(4/6)
196 GCGGG
HaeIII 6 GGCC GG^CC
411 GGCC
HinfI 46 GATTC G^A[ACGT]TC
399 GACTC
HphI 564 GGTGA GGTGA(8/7)
83 TCACC
MaeII 329 ACGT A^CGT
454 ACGT
MflI 182 AGATCT [GA]^GATC[CT]
214 AGATCC
MseI 473 TTAA T^TAA
524 TTAA
MslI 367 CACAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
471 CATTAATGTG
MvnI 195 CGCG CG^CG
226 CGCG
NlaIII 301 CATG CATG^
438 CATG
SfaNI 466 GATGC GCATC(5/9)
170 GCATC
Tru9I 473 TTAA T^TAA
524 TTAA
TseI 424 GCTGC G^C[AT]GC
427 GCTGC
XhoII 182 AGATCT [GA]^GATC[CT]
214 AGATCC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AfaI BsaJI BsiYI BslI
BssKI BstNI DpnII EarI EcoRII
Fnu4HI ItaI Ksp632I MaeIII MboI
MboII MnlI MvaI MwoI NdeII
RsaI Sau3AI ScrFI Tsp45I