Restriction map for
pFN21AB3558
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AlwI AocI ApoI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BfaI BfrI BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BseRI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeIII MamI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SapI SbfI SexAI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 69 GACGTC GACGT^C
AccI 72 GTCGAC GT^[AC][GT]AC
AcyI 69 GACGTC G[GA]^CG[CT]C
AhdI 718 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 763 GTGCAC G^TGCAC
ApaI 223 GGGCCC GGGCC^C
ApaLI 763 GTGCAC G^TGCAC
Asp700I 828 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 718 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 241 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 955 CCCGAG C^[CT]CG[GA]G
BalI 770 TGGCCA TGG^CCA
BanI 583 GGTGCC G^G[CT][GA]CC
BbsI 405 GAAGAC GAAGAC(2/6)
BclI 593 TGATCA T^GATCA
BglI 850 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 524 AGATCT A^GATCT
BpuAI 405 GAAGAC GAAGAC(2/6)
BsaAI 397 TACGTG [CT]AC^GT[GA]
BsaHI 69 GACGTC G[GA]^CG[CT]C
BsgI 622 GTGCAG GTGCAG(16/14)
BsiEI 240 CGACCG CG[GA][CT]^CG
BsoBI 955 CCCGAG C^[CT]CG[GA]G
BsrDI 920 GCAATG GCAATG(2/0)
BsrFI 10 GCCGGT [GA]^CCGG[CT]
BssSI 700 CTCGTG CACGAG(-5/-1)
BstAPI 867 GCAGTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 524 AGATCT [GA]^GATC[CT]
Cfr10I 10 GCCGGT [GA]^CCGG[CT]
Eam1105I 718 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 593 TGATCA T^GATCA
Hin1I 69 GACGTC G[GA]^CG[CT]C
HincII 72 GTCGAC GT[CT]^[GA]AC
HindII 72 GTCGAC GT[CT]^[GA]AC
MflI 524 AGATCT [GA]^GATC[CT]
MluNI 770 TGGCCA TGG^CCA
MscI 770 TGGCCA TGG^CCA
NcoI 116 CCATGG C^CATGG
NspI 736 GCATGT [GA]CATG^[CT]
PflFI 241 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 641 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 948 GAGTC GAGTC(4/5)
PstI 877 CTGCAG CTGCA^G
PvuII 536 CAGCTG CAG^CTG
SalI 72 GTCGAC G^TCGAC
ScaI 791 AGTACT AGT^ACT
SgrAI 9 CGCCGGTG C[GA]^CCGG[CT]G
Tsp509I 483 AATT ^AATT
Tth111I 241 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 641 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 524 AGATCT [GA]^GATC[CT]
XmnI 828 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 270 GTAC GT^AC
792 GTAC
Aor51HI 322 AGCGCT AGC^GCT
488 AGCGCT
AspHI 301 GAGCAC G[AT]GC[AT]^C
763 GTGCAC
BcnI 579 CCGGG CC^[GC]GG
905 CCCGG
BsaI 926 GAGACC GGTCTC(1/5)
958 GAGACC
BsiHKAI 301 GAGCAC G[AT]GC[AT]^C
763 GTGCAC
BsmI 832 GCATTC GAATGC(1/-1)
859 GCATTC
BspMI 92 GCAGGT ACCTGC(4/8)
315 GCAGGT
BstXI 371 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
464 CCATTGGGGTGG
DsaI 116 CCATGG C^C[GA][CT]GG
309 CCACGG
EaeI 770 TGGCCA [CT]^GGCC[GA]
806 CGGCCA
Eco47III 322 AGCGCT AGC^GCT
488 AGCGCT
EcoT14I 116 CCATGG C^C[AT][AT]GG
371 CCAAGG
HaeII 322 AGCGCT [GA]GCGC^[CT]
488 AGCGCT
HgaI 32 GACGC GACGC(5/10)
757 GACGC
MslI 465 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
615 CACTGACGTG
NciI 579 CCGGG CC^[GC]GG
905 CCCGG
NlaIII 117 CATG CATG^
737 CATG
RsaI 270 GTAC GT^AC
792 GTAC
SfcI 513 CTATAG C^T[GA][CT]AG
877 CTGCAG
StyI 116 CCATGG C^C[AT][AT]GG
371 CCAAGG
TfiI 78 GATTC G^A[AT]TC
507 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BanII BbvI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIV
PpuMI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TspRI TthHB8I XcmI