Restriction map for
pFN21AB3558
(Circular) (Six-base) MAPSORT of: pFN21AB3558.seq Check: 9417 from: 1 to: 5704
pFN21AB3558 5704 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2078 3514 278
Size: 53 83 186 936 542 1436 2468
Fragments arranged by size:
2468 1436 936 542 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2078 3047 1937
Size: 141 969 4594
Fragments arranged by size:
4594 969 141
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2827 3767 5151 5419 1632
Size: 1195 940 1384 268 1917
Fragments arranged by size:
1917 1384 1195 940 268
AfeI AGC'GCT
Cuts at: 2329 2495 2329
Size: 166 5538
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 4603 5580 1203
Size: 3400 977 1327
Fragments arranged by size:
3400 1327 977
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 2728 4433 2728
Size: 1705 3999
AleI CACnn'nnGTG
Cuts at: 1166 2624 1166
Size: 1458 4246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2707 2922 1184
Size: 1523 215 3966
Fragments arranged by size:
3966 1523 215
AlwNI CAG_nnn'CTG
Cuts at: 2635 2765 2890 3447 5019 2635
Size: 130 125 557 1572 3320
Fragments arranged by size:
3320 1572 557 130 125
ApaI G_GGCC'C
Cuts at: 2232 2232
Size: 5704
ApaLI G'TGCA_C
Cuts at: 2768 3761 4917 2768
Size: 993 1156 3555
Fragments arranged by size:
3555 1156 993
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 2960 3029 1870
Size: 73 1017 69 4545
Fragments arranged by size:
4545 1017 73 69
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2588 3025 4480 618
Size: 325 217 627 801 437 1455 1842
Fragments arranged by size:
1842 1455 801 627 437 325 217
BanII G_rGCy'C
Cuts at: 1391 2169 2232 2367 3023 1391
Size: 778 63 135 656 4072
Fragments arranged by size:
4072 778 656 135 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2417 928
Size: 971 518 4215
Fragments arranged by size:
4215 971 518
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 2598 1548
Size: 1050 4654
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2861 4315 136
Size: 107 122 71 631 605 1189 1454 1525
Fragments arranged by size:
1525 1454 1189 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2529 5699 2529
Size: 3170 2534
BlpI GC'TnA_GC
Cuts at: 3121 3121
Size: 5704
Bme1580I G_kGCm'C
Cuts at: 1790 2232 2593 2772 2911 3765 4921 1790
Size: 442 361 179 139 854 1156 2573
Fragments arranged by size:
2573 1156 854 442 361 179 139
BmgBI CAC'GTC
Cuts at: 1318 2626 1318
Size: 1308 4396
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3435 4393 442
Size: 416 53 83 399 24 2018 958 1753
Fragments arranged by size:
2018 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2510 2657 2900 2912 2971 4364 1239
Size: 1271 147 243 12 59 1393 2579
Fragments arranged by size:
2579 1393 1271 243 147 59 12
Bpu10I CC'TnA_GC
Cuts at: 2143 2300 2143
Size: 157 5547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4694 4992 3829
Size: 865 298 4541
Fragments arranged by size:
4541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2925 2957 4367 882
Size: 617 1426 32 1410 2219
Fragments arranged by size:
2219 1426 1410 617 32
BsaAI yAC'GTr
Cuts at: 493 1204 2404 3495 493
Size: 711 1200 1091 2702
Fragments arranged by size:
2702 1200 1091 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2075 3511 3893 4528
Size: 53 83 186 936 542 1436 382 635
Cuts at: 4528 275
Size: 1451
Fragments arranged by size:
1451 1436 936 635 542 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5479
Fragments arranged by size:
5479 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5704
BseYI C'CCAG_C
Cuts at: 2169 2507 2543 4907 5403 2169
Size: 338 36 2364 496 2470
Fragments arranged by size:
2470 2364 496 338 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2648 1163
Size: 573 912 4219
Fragments arranged by size:
4219 912 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2248 3154 3915 4943 1540
Size: 275 186 247 906 761 1028 2301
Fragments arranged by size:
2301 1028 906 761 275 247 186
BsiHKAI G_wGCw'C
Cuts at: 2310 2772 3765 3850 4921 2310
Size: 462 993 85 1071 3093
Fragments arranged by size:
3093 1071 993 462 85
BsmI GAATG_Cn'
Cuts at: 2837 2864 3215 3308 2837
Size: 27 351 93 5233
Fragments arranged by size:
5233 351 93 27
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2232 2310 2367 2593 2772 2911
Size: 399 379 63 78 57 226 179 139
Cuts at: 2911 3023 3765 3850 4921 1391
Size: 112 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 226 179 139
112 85 78 63 57
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2311 3059 844
Size: 1244 223 748 3489
Fragments arranged by size:
3489 1244 748 223
BsrBI CCG'CTC
Cuts at: 1394 1823 3591 1394
Size: 429 1768 3507
Fragments arranged by size:
3507 1768 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2932 4199 4373 65
Size: 1183 1684 1267 174 1396
Fragments arranged by size:
1684 1396 1267 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 4348 4535 1700
Size: 110 205 2333 187 2869
Fragments arranged by size:
2869 2333 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 2705 3764 4776 2705
Size: 1059 1012 3633
Fragments arranged by size:
3633 1059 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2878 794
Size: 2084 3620
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstXI CCAn_nnnn'nTGG
Cuts at: 2383 2476 2383
Size: 93 5611
BstYI r'GATC_y
Cuts at: 1972 2529 3034 3401 3786 3803 5255 5341 5353
Size: 557 505 367 385 17 1452 86 12
Cuts at: 5353 5699 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 557 505 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2121 2314 513
Size: 552 939 117 193 3903
Fragments arranged by size:
3903 939 552 193 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2169 2239 2551 487
Size: 683 56 943 70 312 3640
Fragments arranged by size:
3640 943 683 312 70 56
BtsI GCAGTG_nn'
Cuts at: 2657 2911 3220 4014 4034 2657
Size: 254 309 794 20 4327
Fragments arranged by size:
4327 794 309 254 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2414 2449 529
Size: 35 1850 35 3784
Fragments arranged by size:
3784 1850 35 35
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2775 2811 3151
Size: 54 1425 50 275 18 945 36 340
Cuts at: 3151 4040 5669 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 945 889 340 275 54 50
43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3634 1353
Size: 2281 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2163 2946 4291 4665 4811 387
Size: 1064 138 574 783 1345 374 146 1280
Fragments arranged by size:
1345 1280 1064 783 574 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 2657 2827 2900 2912 2971 3767
Size: 393 878 147 170 73 12 59 796
Cuts at: 3767 4364 5151 5419 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 878 796 787 597 393 268 170
147 73 59 12
EcoO109I rG'GnC_Cy
Cuts at: 2100 2208 2228 2229 2722 2887 2982 2100
Size: 108 20 1 493 165 95 4822
Fragments arranged by size:
4822 493 165 108 95 20 1
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 5704
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 2331 2497 4851 1678
Size: 653 166 2354 2531
Fragments arranged by size:
2531 2354 653 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2714 2746 2786 2818 2922
Size: 32 135 32 1142 32 40 32 104
Cuts at: 2922 2954 4345 4377 4419 4451 1373
Size: 32 1391 32 42 32 2626
Fragments arranged by size:
2626 1391 1142 135 104 42 40 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2079 3048 3294 669
Size: 988 281 141 969 246 3079
Fragments arranged by size:
3079 988 969 281 246 141
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2078 2637 4818 5002 1305
Size: 773 559 2181 184 2007
Fragments arranged by size:
2181 2007 773 559 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2777 10
Size: 54 1425 343 945 2937
Fragments arranged by size:
2937 1425 945 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 2624 3662 4021 4180 518
Size: 648 1308 150 1038 359 159 2042
Fragments arranged by size:
2042 1308 1038 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2045 2138 2543 3120 3797 4945 5190 1606
Size: 439 93 405 577 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 577 439 405 245 93
NcoI C'CATG_G
Cuts at: 513 1065 2004 2121 513
Size: 552 939 117 4096
Fragments arranged by size:
4096 939 552 117
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 2745 3062 4607 1132
Size: 97 1516 317 1545 2229
Fragments arranged by size:
2229 1545 1516 317 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2652 1108
Size: 1544 4160
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2100 2208 2722 2887 2100
Size: 108 514 165 4917
Fragments arranged by size:
4917 514 165 108
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PspOMI G'GGCC_C
Cuts at: 2228 2228
Size: 5704
PstI C_TGCA'G
Cuts at: 830 2886 3056 830
Size: 2056 170 3478
Fragments arranged by size:
3478 2056 170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
PvuII CAG'CTG
Cuts at: 2543 2543
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 2077 3046 1936
Size: 141 969 4594
Fragments arranged by size:
4594 969 141
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 2798 3952 5664 2798
Size: 1154 1712 2838
Fragments arranged by size:
2838 1712 1154
SfcI C'TryA_G
Cuts at: 826 1045 2518 2882 3052 4187 4868 5059 826
Size: 219 1473 364 170 1135 681 191 1471
Fragments arranged by size:
1473 1471 1135 681 364 219 191 170
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 2015 1700
Size: 315 5389
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3808 4709 4971 820
Size: 197 926 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5704
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2376 3143 513
Size: 552 939 117 255 767 3074
Fragments arranged by size:
3074 939 767 552 255 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3731 3748 3901 4086 4531 325
Size: 1255 2151 17 153 185 445 1498
Fragments arranged by size:
2151 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2796 3950 5662
Size: 274 80 33 51 1443 819 1154 1712
Cuts at: 5662 96
Size: 138
Fragments arranged by size:
1712 1443 1154 819 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5664 5696 5664
Size: 32 5672
Tth111I GACn'n_nGTC
Cuts at: 1316 2249 1316
Size: 933 4771
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2383 2473 2653 2924 2383
Size: 90 180 271 5163
Fragments arranged by size:
5163 271 180 90
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 2837 3833 2837
Size: 996 4708
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2076 3512 276
Size: 53 83 186 936 542 1436 2468
Fragments arranged by size:
2468 1436 936 542 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI