Restriction map for
pFN21AB7253
##################################
# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
Bsp1286I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HaeII HapII Hin1I HindIII HpaI
HpaII KasI KpnI KspI MaeIII
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StyI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AlwI 1106 GGATC GGATC(4/5)
BalI 996 TGGCCA TGG^CCA
BciVI 1020 GTATCC GTATCC(6/5)
BclI 1056 TGATCA T^GATCA
BpmI 17 CTCCAG CTGGAG(16/14)
BsaJI 276 CCCTGG C^C[ACGT][ACGT]GG
BstAPI 937 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1106 GGATCT [GA]^GATC[CT]
CfoI 117 GCGC GCG^C
DraII 135 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1192 GACAAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 996 TGGCCA [CT]^GGCC[GA]
EarI 977 CTCTTC CTCTTC(1/4)
EcoO109I 135 GGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 1056 TGATCA T^GATCA
HgaI 906 GACGC GACGC(5/10)
HhaI 117 GCGC GCG^C
HinP1I 117 GCGC G^CGC
HincII 505 GTCAAC GT[CT]^[GA]AC
HindII 505 GTCAAC GT[CT]^[GA]AC
Ksp632I 977 CTCTTC CTCTTC(1/4)
MaeII 326 ACGT A^CGT
MflI 1106 GGATCT [GA]^GATC[CT]
MluNI 996 TGGCCA TGG^CCA
MscI 996 TGGCCA TGG^CCA
MslI 46 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 574 GCTAGC G^CTAGC
NspI 509 ACATGC [GA]CATG^[CT]
PpuMI 135 GGGACCT [GA]G^G[AT]CC[CT]
SexAI 81 ACCAGGT A^CC[AT]GGT
SmlI 725 CTCAAG C^T[CT][GA]AG
SspI 808 AATATT AAT^ATT
StuI 1079 AGGCCT AGG^CCT
XhoII 1106 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 5 CCGC CCGC(-3/-1)
26 CCGC
AluI 21 AGCT AG^CT
1131 AGCT
Asp700I 338 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
620 GAAATAATTC
BbsI 342 GTCTTC GAAGAC(2/6)
1005 GTCTTC
BbvI 937 GCAGC GCAGC(8/12)
202 GCTGC
BpuAI 342 GTCTTC GAAGAC(2/6)
1005 GTCTTC
BsgI 948 CTGCAC GTGCAG(16/14)
935 GTGCAG
BsmFI 135 GGGAC GGGAC(10/14)
904 GGGAC
BsmI 314 GAATGC GAATGC(1/-1)
570 GAATGC
Bsp1407I 87 TGTACA T^GTACA
1036 TGTACA
BsrGI 87 TGTACA T^GTACA
1036 TGTACA
DpnII 1057 GATC ^GATC
1107 GATC
FauI 4 CCCGC CCCGC(4/6)
25 CCCGC
Fnu4HI 202 GCTGC GC^[ACGT]GC
937 GCAGC
ItaI 202 GCTGC GC^[ACGT]GC
937 GCAGC
MboI 1057 GATC ^GATC
1107 GATC
NdeII 1057 GATC ^GATC
1107 GATC
NlaIV 135 GGGACC GG[ACGT]^[ACGT]CC
374 GGAACC
Sau3AI 1057 GATC ^GATC
1107 GATC
SspBI 87 TGTACA T^GTACA
1036 TGTACA
TaqI 423 TCGA T^CGA
831 TCGA
TseI 202 GCTGC G^C[AT]GC
937 GCAGC
TthHB8I 423 TCGA T^CGA
831 TCGA
XmnI 338 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
620 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI ApoI AvaII BfaI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
FokI HaeIII HinfI HphI MaeI
MboII MnlI MseI MvaI MwoI
NlaIII PflMI PstI RsaI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
Tsp509I TspRI Van91I