(Circular) (Six-base) MAPSORT of: pFN21AB7253.seq Check: 1894 from: 1 to: 5962 pFN21AB7253 5962 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2901 4025 5409 5677 1632 Size: 1269 1124 1384 268 1917 Fragments arranged by size: 1917 1384 1269 1124 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2968 1184 Size: 1784 4178 AlwNI CAG_nnn'CTG Cuts at: 3705 5277 3705 Size: 1572 4390 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 2551 2562 2635 2758 3036 3271 3497 3649 Size: 1058 11 73 123 278 235 226 152 Cuts at: 3649 5758 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1058 278 235 226 152 123 73 11 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 3287 1870 Size: 73 1344 4545 Fragments arranged by size: 4545 1344 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3283 4738 618 Size: 325 217 627 1496 1455 1842 Fragments arranged by size: 1842 1496 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3281 1391 Size: 1890 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2340 3003 928 Size: 971 441 663 3887 Fragments arranged by size: 3887 971 663 441 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4153 4187 1903 Size: 34 50 34 2132 34 3678 Fragments arranged by size: 3678 2132 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3036 3854 5064 3036 Size: 818 1210 3934 Fragments arranged by size: 3934 1210 818 BclI T'GATC_A Cuts at: 1548 3061 1548 Size: 1513 4449 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 4023 5179 1790 Size: 2233 1156 2573 Fragments arranged by size: 2573 2233 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3014 3693 4651 Size: 416 53 83 399 24 1597 679 958 Cuts at: 4651 442 Size: 1753 Fragments arranged by size: 1753 1597 958 679 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2583 1056 Size: 1527 4435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2007 4622 1239 Size: 768 2615 2579 Fragments arranged by size: 2615 2579 768 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2715 4087 4952 5250 2715 Size: 1372 865 298 3427 Fragments arranged by size: 3427 1372 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4625 882 Size: 617 3126 2219 Fragments arranged by size: 3126 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3753 493 Size: 711 2549 2702 Fragments arranged by size: 2702 2549 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5737 Fragments arranged by size: 5737 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5962 BseYI C'CCAG_C Cuts at: 5165 5661 5165 Size: 496 5466 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2938 2961 1163 Size: 573 1202 23 4164 Fragments arranged by size: 4164 1202 573 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3412 4173 5201 1540 Size: 275 186 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4023 4108 5179 4023 Size: 85 1071 4806 Fragments arranged by size: 4806 1071 85 BsmI GAATG_Cn' Cuts at: 2325 2581 3473 3566 2325 Size: 256 892 93 4721 Fragments arranged by size: 4721 892 256 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3281 4023 4108 5179 1391 Size: 399 1491 742 85 1071 2174 Fragments arranged by size: 2174 1491 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 3851 5581 3851 Size: 1730 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3317 844 Size: 2473 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4606 4793 1700 Size: 110 2796 187 2869 Fragments arranged by size: 2869 2796 187 110 BsrGI T'GTAC_A Cuts at: 96 2092 3041 96 Size: 1996 949 3017 Fragments arranged by size: 3017 1996 949 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 2948 794 Size: 2154 3808 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstYI r'GATC_y Cuts at: 1972 3111 3292 3659 4044 4061 5513 5599 5611 Size: 1139 181 367 385 17 1452 86 12 Cuts at: 5611 5957 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1139 385 367 346 181 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5410 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 3252 3259 3478 4272 4292 3252 Size: 7 219 794 20 4922 Fragments arranged by size: 4922 794 219 20 7 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2763 2798 529 Size: 35 2199 35 3693 Fragments arranged by size: 3693 2199 35 35 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3203 4969 809 Size: 507 1887 1766 1802 Fragments arranged by size: 1887 1802 1766 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3001 3409 4298 Size: 54 1425 50 275 18 1171 408 889 Cuts at: 4298 5927 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1171 889 408 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2988 3892 1353 Size: 1635 904 3423 Fragments arranged by size: 3423 1635 904 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2901 4025 4622 5409 5677 1239 Size: 393 375 894 1124 597 787 268 1524 Fragments arranged by size: 1524 1124 894 787 597 393 375 268 EcoO109I rG'GnC_Cy Cuts at: 2141 2141 Size: 5962 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2364 2364 Size: 5962 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 5109 1678 Size: 3431 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2591 2623 4603 4635 4677 Size: 32 135 32 1019 32 1980 32 42 Cuts at: 4677 4709 1373 Size: 32 2626 Fragments arranged by size: 2626 1980 1019 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2512 3306 3552 669 Size: 988 281 574 794 246 3079 Fragments arranged by size: 3079 988 794 574 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5076 5260 1305 Size: 3771 184 2007 Fragments arranged by size: 3771 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3003 10 Size: 54 1425 343 1171 2969 Fragments arranged by size: 2969 1425 1171 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2055 3920 4279 4438 518 Size: 648 889 1865 359 159 2042 Fragments arranged by size: 2042 1865 889 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3378 4055 5203 5448 1606 Size: 1772 677 1148 245 2120 Fragments arranged by size: 2120 1772 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5410 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 2579 1052 Size: 1527 4435 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2518 3320 4865 1132 Size: 97 1289 802 1545 2229 Fragments arranged by size: 2229 1545 1289 802 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 2203 2276 2360 1108 Size: 1095 73 84 4710 Fragments arranged by size: 4710 1095 84 73 PfoI T'CCnGG_A Cuts at: 2620 2620 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2141 2141 Size: 5962 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 2487 3532 2487 Size: 1045 4917 PstI C_TGCA'G Cuts at: 830 3157 3169 3247 3314 830 Size: 2327 12 78 67 3478 Fragments arranged by size: 3478 2327 78 67 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SexAI A'CCwGG_T Cuts at: 2086 2086 Size: 5962 SfcI C'TryA_G Cuts at: 826 1045 2305 3153 3165 3243 3310 4445 5126 Size: 219 1260 848 12 78 67 1135 681 Cuts at: 5126 5317 826 Size: 191 1471 Fragments arranged by size: 1471 1260 1135 848 681 219 191 78 67 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2730 4066 4967 5229 820 Size: 197 926 787 1336 901 262 1553 Fragments arranged by size: 1553 1336 926 901 787 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 2815 3886 5 Size: 47 2763 1071 2081 Fragments arranged by size: 2763 2081 1071 47 StuI AGG'CCT Cuts at: 1517 3086 1517 Size: 1569 4393 StyI C'CwwG_G Cuts at: 513 1065 3401 513 Size: 552 2336 3074 Fragments arranged by size: 3074 2336 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2406 3003 3989 4006 4159 4344 4789 Size: 1255 826 597 986 17 153 185 445 Cuts at: 4789 325 Size: 1498 Fragments arranged by size: 1498 1255 986 826 597 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2092 2309 3041 Size: 274 80 33 51 1443 115 217 732 Cuts at: 3041 3116 4208 5920 96 Size: 75 1092 1712 138 Fragments arranged by size: 1712 1443 1092 732 274 217 138 115 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 2347 2629 4091 2347 Size: 282 1462 4218 Fragments arranged by size: 4218 1462 282 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI