Restriction map for
pFN21AB6862
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NdeI NgoMIV NheI
NotI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SalI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AclI 382 AACGTT AA^CGTT
AocI 540 CCTAAGG CC^T[ACGT]AGG
Asp700I 480 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 296 GGATCC G^GATCC
BbeI 193 GGCGCC GGCGC^C
BsaI 184 GAGACC GGTCTC(1/5)
BsgI 334 CTGCAC GTGCAG(16/14)
BsmAI 184 GAGAC GTCTC(1/5)
BsmFI 549 GTCCC GGGAC(10/14)
BspMI 514 ACCTGC ACCTGC(4/8)
BsrBI 577 GAGCGG CCGCTC(-3/-3)
BstXI 270 CCAGGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 296 GGATCC [GA]^GATC[CT]
Bsu36I 540 CCTAAGG CC^T[ACGT]AGG
DpnII 297 GATC ^GATC
DrdI 160 GACAAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 484 CTCTTC CTCTTC(1/4)
Eco81I 540 CCTAAGG CC^T[ACGT]AGG
EcoNI 561 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 193 GGCGCC [GA]GCGC^[CT]
HinfI 547 GAGTC G^A[ACGT]TC
KasI 193 GGCGCC G^GCGCC
Ksp632I 484 CTCTTC CTCTTC(1/4)
KspI 118 CCGCGG CCGC^GG
MaeIII 376 GTCAC ^GT[ACGT]AC
MboI 297 GATC ^GATC
MboII 485 TCTTC GAAGA(8/7)
MflI 296 GGATCC [GA]^GATC[CT]
NarI 193 GGCGCC GG^CGCC
NcoI 137 CCATGG C^CATGG
NdeII 297 GATC ^GATC
NruI 368 TCGCGA TCG^CGA
PleI 547 GAGTC GAGTC(4/5)
Psp1406I 382 AACGTT AA^CGTT
SacII 118 CCGCGG CCGC^GG
SapI 483 GCTCTTC GCTCTTC(1/4)
Sau3AI 297 GATC ^GATC
SbfI 231 CCTGCAGG CCTGCA^GG
SexAI 560 ACCTGGT A^CC[AT]GGT
SfoI 193 GGCGCC GGC^GCC
SmlI 58 CTCAAG C^T[CT][GA]AG
Sse8387I 231 CCTGCAGG CCTGCA^GG
StuI 330 AGGCCT AGG^CCT
Tsp45I 376 GTCAC ^GT[GC]AC
TspRI 443 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 296 GGATCC [GA]^GATC[CT]
XmnI 480 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 408 GTAC GT^AC
435 GTAC
AlwI 297 GATCC GGATC(4/5)
296 GGATC
AspHI 55 GAGCTC G[AT]GC[AT]^C
199 GAGCTC
BanI 87 GGTGCC G^G[CT][GA]CC
193 GGCGCC
BcnI 245 CCGGG CC^[GC]GG
354 CCGGG
BsiHKAI 55 GAGCTC G[AT]GC[AT]^C
199 GAGCTC
BsrI 509 ACTGG ACTGG(1/-1)
552 CCAGT
DdeI 311 CTCAG C^T[ACGT]AG
541 CTAAG
DsaI 118 CCGCGG C^C[GA][CT]GG
137 CCATGG
EaeI 242 TGGCCG [CT]^GGCC[GA]
260 CGGCCA
EcoT14I 137 CCATGG C^C[AT][AT]GG
146 CCTTGG
HapII 245 CCGG C^CGG
354 CCGG
HgaI 16 GACGC GACGC(5/10)
142 GACGC
HpaII 245 CCGG C^CGG
354 CCGG
HphI 155 TCACC GGTGA(8/7)
203 TCACC
MaeII 212 ACGT A^CGT
383 ACGT
MspI 245 CCGG C^CGG
354 CCGG
NciI 245 CCGGG CC^[GC]GG
354 CCGGG
NlaIII 138 CATG CATG^
225 CATG
PpuMI 416 AGGTCCT [GA]G^G[AT]CC[CT]
569 AGGACCT
PstI 232 CTGCAG CTGCA^G
253 CTGCAG
PvuII 250 CAGCTG CAG^CTG
290 CAGCTG
RsaI 408 GTAC GT^AC
435 GTAC
SacI 55 GAGCTC GAGCT^C
199 GAGCTC
SfcI 232 CTGCAG C^T[GA][CT]AG
253 CTGCAG
StyI 137 CCATGG C^C[AT][AT]GG
146 CCTTGG
XcmI 137 CCATGGACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
499 CCAGCCTGGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanII BbvI BmyI BpmI BsaHI
BsaJI BseRI BsiYI BslI Bsp1286I
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HhaI Hin1I HinP1I ItaI MnlI
MspA1I MvaI MvnI MwoI NlaIV
Sau96I ScrFI SfaNI TseI