(Circular) (Six-base) MAPSORT of: pFN21AB6862.seq Check: 1510 from: 1 to: 5290 pFN21AB6862 5290 bp With 182 enzymes: SgfI * October 20, 2009 10:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3100 278 Size: 53 83 186 936 1564 2468 Fragments arranged by size: 2468 1564 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2633 1937 Size: 696 4594 Acc65I G'GTAC_C Cuts at: 2611 2611 Size: 5290 AclI AA'CG_TT Cuts at: 1563 2388 3417 3790 1563 Size: 825 1029 373 3063 Fragments arranged by size: 3063 1029 825 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 2476 3353 4737 5005 1632 Size: 467 377 877 1384 268 1917 Fragments arranged by size: 1917 1384 877 467 377 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5093 AflIII A'CryG_T Cuts at: 1203 4189 5166 1203 Size: 2986 977 1327 Fragments arranged by size: 2986 1327 977 AgeI A'CCGG_T Cuts at: 4121 4121 Size: 5290 AhdI GACnn_n'nnGTC Cuts at: 4019 4019 Size: 5290 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5290 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2379 2546 1184 Size: 1195 167 3928 Fragments arranged by size: 3928 1195 167 AlwNI CAG_nnn'CTG Cuts at: 3033 4605 3033 Size: 1572 3718 ApaLI G'TGCA_C Cuts at: 3347 4503 3347 Size: 1156 4134 ApoI r'AATT_y Cuts at: 1493 2599 2825 2977 5086 1493 Size: 1106 226 152 2109 1697 Fragments arranged by size: 2109 1697 1106 226 152 AseI AT'TA_AT Cuts at: 160 1032 3844 160 Size: 872 2812 1606 Fragments arranged by size: 2812 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5290 AvaI C'yCGr_G Cuts at: 1870 1943 2615 1870 Size: 73 672 4545 Fragments arranged by size: 4545 672 73 BamHI G'GATC_C Cuts at: 2301 2620 2987 2301 Size: 319 367 4604 Fragments arranged by size: 4604 367 319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2092 2198 2611 4066 618 Size: 325 217 627 305 106 413 1455 1842 Fragments arranged by size: 1842 1455 627 413 325 305 217 106 BanII G_rGCy'C Cuts at: 1391 2064 2208 2282 2463 2609 1391 Size: 673 144 74 181 146 4072 Fragments arranged by size: 4072 673 181 146 144 74 BbeI G_GCGC'C Cuts at: 2202 2202 Size: 5290 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3481 3515 1903 Size: 34 1544 34 3678 Fragments arranged by size: 3678 1544 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3182 4392 3182 Size: 1210 4080 BclI T'GATC_A Cuts at: 1548 1548 Size: 5290 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3901 136 Size: 107 122 71 631 605 2229 1525 Fragments arranged by size: 2229 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5285 5285 Size: 5290 BlpI GC'TnA_GC Cuts at: 2707 2707 Size: 5290 Bme1580I G_kGCm'C Cuts at: 1790 3351 4507 1790 Size: 1561 1156 2573 Fragments arranged by size: 2573 1561 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5290 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2551 3021 3979 Size: 416 53 83 399 24 1134 470 958 Cuts at: 3979 442 Size: 1753 Fragments arranged by size: 1753 1134 958 470 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5290 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2207 2304 2522 2600 3950 1239 Size: 821 147 97 218 78 1350 2579 Fragments arranged by size: 2579 1350 821 218 147 97 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2048 3415 4280 4578 2048 Size: 1367 865 298 2760 Fragments arranged by size: 2760 1367 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2183 3953 882 Size: 617 684 1770 2219 Fragments arranged by size: 2219 1770 684 617 BsaAI yAC'GTr Cuts at: 493 1204 3081 493 Size: 711 1877 2702 Fragments arranged by size: 2702 1877 711 BsaBI GATnn'nnATC Cuts at: 5284 5284 Size: 5290 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 2148 2199 3097 Size: 53 83 186 936 489 126 51 898 Cuts at: 3097 3479 4114 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 898 635 489 382 186 126 83 53 51 BsaWI w'CCGG_w Cuts at: 3723 4121 4395 4542 4953 3723 Size: 398 274 147 411 4060 Fragments arranged by size: 4060 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 1178 Size: 30 165 30 1140 30 3895 Fragments arranged by size: 3895 1140 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2304 2525 1186 Size: 886 232 221 3951 Fragments arranged by size: 3951 886 232 221 BseYI C'CCAG_C Cuts at: 2297 4493 4989 2297 Size: 2196 496 2598 Fragments arranged by size: 2598 2196 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2324 1163 Size: 573 588 4129 Fragments arranged by size: 4129 588 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2740 3501 4529 1540 Size: 275 186 739 761 1028 2301 Fragments arranged by size: 2301 1028 761 739 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 2208 3351 3436 4507 2064 Size: 144 1143 85 1071 2847 Fragments arranged by size: 2847 1143 1071 144 85 BsmI GAATG_Cn' Cuts at: 2801 2894 2801 Size: 93 5197 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2208 2282 2463 2609 3351 3436 Size: 399 274 144 74 181 146 742 85 Cuts at: 3436 4507 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 274 181 146 144 85 74 BspEI T'CCGG_A Cuts at: 4953 4953 Size: 5290 BspHI T'CATG_A Cuts at: 3179 4909 3179 Size: 1730 3560 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2528 2645 844 Size: 1684 117 3489 Fragments arranged by size: 3489 1684 117 BsrBI CCG'CTC Cuts at: 1394 1823 2584 3177 1394 Size: 429 761 593 3507 Fragments arranged by size: 3507 761 593 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3785 3959 65 Size: 1183 2537 174 1396 Fragments arranged by size: 2537 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3934 4121 1700 Size: 110 2124 187 2869 Fragments arranged by size: 2869 2124 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5290 BssHII G'CGCG_C Cuts at: 1925 2011 2013 2015 2130 1925 Size: 86 2 2 115 5085 Fragments arranged by size: 5085 115 86 2 2 BssSI C'ACGA_G Cuts at: 3350 4362 3350 Size: 1012 4278 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5290 BstBI TT'CG_AA Cuts at: 4094 4094 Size: 5290 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5290 BstXI CCAn_nnnn'nTGG Cuts at: 2282 2282 Size: 5290 BstYI r'GATC_y Cuts at: 1972 2301 2620 2987 3372 3389 4841 4927 4939 Size: 329 319 367 385 17 1452 86 12 Cuts at: 4939 5285 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 329 319 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2546 2546 Size: 5290 BtgI C'CryG_G Cuts at: 513 1065 2004 2123 2142 513 Size: 552 939 119 19 3661 Fragments arranged by size: 3661 939 552 119 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4551 Fragments arranged by size: 4551 683 56 BtsI GCAGTG_nn' Cuts at: 2454 2806 3600 3620 2454 Size: 352 794 20 4124 Fragments arranged by size: 4124 794 352 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5290 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5255 DraI TTT'AAA Cuts at: 2594 2594 Size: 5290 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2171 4297 809 Size: 507 855 2126 1802 Fragments arranged by size: 2126 1802 855 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2247 2265 2737 Size: 54 1425 50 275 18 417 18 472 Cuts at: 2737 3626 5255 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 472 417 275 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2737 1537 Size: 275 925 4090 Fragments arranged by size: 4090 925 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2495 3220 1353 Size: 1142 725 3423 Fragments arranged by size: 3423 1142 725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3877 4251 4397 387 Size: 1064 138 2288 374 146 1280 Fragments arranged by size: 2288 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2062 2206 2062 Size: 144 5146 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2099 2207 2304 2476 2522 2600 Size: 393 428 39 108 97 172 46 78 Cuts at: 2600 3353 3950 4737 5005 1239 Size: 753 597 787 268 1524 Fragments arranged by size: 1524 787 753 597 428 393 268 172 108 97 78 46 39 EcoNI CCTnn'n_nnAGG Cuts at: 2570 2570 Size: 5290 EcoO109I rG'GnC_Cy Cuts at: 2308 2422 2542 2575 2308 Size: 114 120 33 5023 Fragments arranged by size: 5023 120 114 33 EcoRI G'AATT_C Cuts at: 2599 2977 2599 Size: 378 4912 FspI TGC'GCA Cuts at: 3796 3796 Size: 5290 HaeII r_GCGC'y Cuts at: 1678 2202 4437 1678 Size: 524 2235 2531 Fragments arranged by size: 2531 2235 524 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2543 2575 3931 3963 4005 Size: 32 135 32 971 32 1356 32 42 Cuts at: 4005 4037 1373 Size: 32 2626 Fragments arranged by size: 2626 1356 971 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2634 2880 669 Size: 988 281 696 246 3079 Fragments arranged by size: 3079 988 696 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5290 HpaI GTT'AAC Cuts at: 2880 2880 Size: 5290 KasI G'GCGC_C Cuts at: 2198 2198 Size: 5290 KpnI G_GTAC'C Cuts at: 2615 2615 Size: 5290 MfeI C'AATT_G Cuts at: 2889 2889 Size: 5290 MluI A'CGCG_T Cuts at: 5166 5166 Size: 5290 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4404 4588 1305 Size: 3099 184 2007 Fragments arranged by size: 3099 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3468 Fragments arranged by size: 3468 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3248 3607 3766 518 Size: 648 2082 359 159 2042 Fragments arranged by size: 2082 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2125 2257 2297 2706 3383 4531 4776 Size: 492 27 132 40 409 677 1148 245 Cuts at: 4776 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 492 409 245 132 40 27 NarI GG'CG_CC Cuts at: 2199 2199 Size: 5290 NcoI C'CATG_G Cuts at: 513 1065 2004 2142 513 Size: 552 939 138 3661 Fragments arranged by size: 3661 939 552 138 NdeI CA'TA_TG Cuts at: 387 387 Size: 5290 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5290 NotI GC'GGCC_GC Cuts at: 2737 2737 Size: 5290 NruI TCG'CGA Cuts at: 2375 2375 Size: 5290 NspI r_CATG'y Cuts at: 1132 1229 2648 4193 1132 Size: 97 1419 1545 2229 Fragments arranged by size: 2229 1545 1419 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5290 PciI A'CATG_T Cuts at: 4189 4189 Size: 5290 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5290 PfoI T'CCnGG_A Cuts at: 2028 2028 Size: 5290 PmeI GTTT'AAAC Cuts at: 2594 2594 Size: 5290 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3359 3391 4872 4904 3359 Size: 32 1481 32 3745 Fragments arranged by size: 3745 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2422 2575 2422 Size: 153 5137 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5290 PsiI TTA'TAA Cuts at: 2860 2860 Size: 5290 PstI C_TGCA'G Cuts at: 830 2241 2262 2642 830 Size: 1411 21 380 3478 Fragments arranged by size: 3478 1411 380 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5290 PvuII CAG'CTG Cuts at: 2257 2297 2257 Size: 40 5250 SacI G_AGCT'C Cuts at: 2064 2208 2064 Size: 144 5146 SacII CC_GC'GG Cuts at: 2126 2126 Size: 5290 SalI G'TCGA_C Cuts at: 1936 2632 1936 Size: 696 4594 SapI GCTCTTCn'nnn_ Cuts at: 2495 2495 Size: 5290 SbfI CC_TGCA'GG Cuts at: 2241 2642 2241 Size: 401 4889 ScaI AGT'ACT Cuts at: 3538 5250 3538 Size: 1712 3578 SexAI A'CCwGG_T Cuts at: 2565 2565 Size: 5290 SfcI C'TryA_G Cuts at: 826 1045 2237 2258 2638 3773 4454 4645 826 Size: 219 1192 21 380 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 681 380 219 191 21 SfoI GGC'GCC Cuts at: 2200 2200 Size: 5290 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5290 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5290 SmaI CCC'GGG Cuts at: 1872 2617 1872 Size: 745 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2063 3394 4295 4557 820 Size: 197 926 120 1331 901 262 1553 Fragments arranged by size: 1553 1331 926 901 262 197 120 SnaBI TAC'GTA Cuts at: 493 3081 493 Size: 2588 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5290 SphI G_CATG'C Cuts at: 1132 2648 1132 Size: 1516 3774 SspI AAT'ATT Cuts at: 5 52 3214 5 Size: 47 3162 2081 Fragments arranged by size: 3162 2081 47 StuI AGG'CCT Cuts at: 1517 2337 1517 Size: 820 4470 StyI C'CwwG_G Cuts at: 513 1065 2004 2142 2151 2729 513 Size: 552 939 138 9 578 3074 Fragments arranged by size: 3074 939 578 552 138 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3317 3334 3487 3672 4117 325 Size: 1255 1737 17 153 185 445 1498 Fragments arranged by size: 1737 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3536 5248 96 Size: 274 80 33 51 1443 1559 1712 138 Fragments arranged by size: 1712 1559 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5250 5282 5250 Size: 32 5258 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5290 XbaI T'CTAG_A Cuts at: 2626 2626 Size: 5290 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2511 2149 Size: 362 4928 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5290 XmaI C'CCGG_G Cuts at: 1870 2615 1870 Size: 745 4545 XmnI GAAnn'nnTTC Cuts at: 2489 3419 2489 Size: 930 4360 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3098 276 Size: 53 83 186 936 1564 2468 Fragments arranged by size: 2468 1564 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SfiI SrfI SwaI