################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI BstYI Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MroI MseI MslI MspA1I MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI Tru9I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 394 GTATAC GT^[AC][GT]AC AccII 63 CGCG CG^CG AcsI 85 AAATTT [GA]^AATT[CT] AluI 330 AGCT AG^CT ApoI 85 AAATTT [GA]^AATT[CT] BalI 141 TGGCCA TGG^CCA BclI 264 TGATCA T^GATCA BpmI 352 CTCCAG CTGGAG(16/14) BsaJI 144 CCATGG C^C[ACGT][ACGT]GG BseRI 241 GAGGAG GAGGAG(10/8) Bsp1407I 77 TGTACA T^GTACA BsrGI 77 TGTACA T^GTACA BssKI 30 CCAGG ^CC[ACGT]GG Bst1107I 394 GTATAC GTA^TAC BstF5I 285 GGATG GGATG(2/0) BstNI 30 CCAGG CC^[AT]GG BstUI 63 CGCG CG^CG BstZ17I 394 GTATAC GTA^TAC Cac8I 64 GCGAGC GC[ACGT]^[ACGT]GC CfoI 62 GCGC GCG^C DdeI 91 CTAAG C^T[ACGT]AG DsaI 144 CCATGG C^C[GA][CT]GG EcoRII 30 CCAGG ^CC[AT]GG EcoT14I 144 CCATGG C^C[AT][AT]GG FauI 44 CCCGC CCCGC(4/6) FbaI 264 TGATCA T^GATCA FokI 285 GGATG GGATG(9/13) HhaI 62 GCGC GCG^C HinP1I 62 GCGC G^CGC MaeIII 169 GTGAC ^GT[ACGT]AC MluNI 141 TGGCCA TGG^CCA MscI 141 TGGCCA TGG^CCA MvaI 30 CCAGG CC^[AT]GG MvnI 63 CGCG CG^CG MwoI 20 GCCGTCGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 144 CCATGG C^CATGG ScrFI 30 CCAGG CC^[ACGT]GG SfaNI 50 GCATC GCATC(5/9) SfcI 368 CTGTAG C^T[GA][CT]AG SspBI 77 TGTACA T^GTACA SspI 412 AATATT AAT^ATT StyI 144 CCATGG C^C[AT][AT]GG Tsp45I 169 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 45 CCGC CCGC(-3/-1) 48 CCGC AfaI 78 GTAC GT^AC 175 GTAC AspHI 3 GAGCAC G[AT]GC[AT]^C 312 GAGCAC BbvI 255 GCAGC GCAGC(8/12) 26 GCTGC BsiHKAI 3 GAGCAC G[AT]GC[AT]^C 312 GAGCAC BsiYI 192 CCCTGCTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 193 CCTGCTACGGG BslI 192 CCCTGCTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 193 CCTGCTACGGG BsmFI 344 GGGAC GGGAC(10/14) 42 GTCCC DpnII 223 GATC ^GATC 265 GATC EaeI 18 TGGCCG [CT]^GGCC[GA] 141 TGGCCA HaeIII 19 GGCC GG^CC 142 GGCC HinfI 213 GAATC G^A[ACGT]TC 273 GAATC HphI 168 GGTGA GGTGA(8/7) 309 GGTGA MboI 223 GATC ^GATC 265 GATC MboII 100 GAAGA GAAGA(8/7) 363 TCTTC NdeII 223 GATC ^GATC 265 GATC NlaIII 145 CATG CATG^ 178 CATG RsaI 78 GTAC GT^AC 175 GTAC Sau3AI 223 GATC ^GATC 265 GATC TfiI 213 GAATC G^A[AT]TC 273 GAATC TseI 26 GCTGC G^C[AT]GC 255 GCAGC Tsp509I 86 AATT ^AATT 154 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### BmyI Bsp1286I Fnu4HI ItaI MnlI