(Circular) (Six-base) MAPSORT of: pFN21AE6147.seq Check: 759 from: 1 to: 5140 pFN21AE6147 5140 bp With 182 enzymes: SgfI * December 8, 2014 13:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2950 278 Size: 53 83 186 936 1414 2468 Fragments arranged by size: 2468 1414 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2400 2483 1937 Size: 463 83 4594 Fragments arranged by size: 4594 463 83 Acc65I G'GTAC_C Cuts at: 2461 2461 Size: 5140 AclI AA'CG_TT Cuts at: 1563 3267 3640 1563 Size: 1704 373 3063 Fragments arranged by size: 3063 1704 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2047 3203 4587 4855 1632 Size: 415 1156 1384 268 1917 Fragments arranged by size: 1917 1384 1156 415 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4943 AflIII A'CryG_T Cuts at: 1203 4039 5016 1203 Size: 2836 977 1327 Fragments arranged by size: 2836 1327 977 AgeI A'CCGG_T Cuts at: 3971 3971 Size: 5140 AhdI GACnn_n'nnGTC Cuts at: 3869 3869 Size: 5140 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5140 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5140 AlwNI CAG_nnn'CTG Cuts at: 2883 4455 2883 Size: 1572 3568 ApaLI G'TGCA_C Cuts at: 3197 4353 3197 Size: 1156 3984 ApoI r'AATT_y Cuts at: 1493 2090 2449 2675 2827 4936 1493 Size: 597 359 226 152 2109 1697 Fragments arranged by size: 2109 1697 597 359 226 152 AseI AT'TA_AT Cuts at: 160 1032 3694 160 Size: 872 2662 1606 Fragments arranged by size: 2662 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5140 AvaI C'yCGr_G Cuts at: 1870 1943 2465 1870 Size: 73 522 4545 Fragments arranged by size: 4545 522 73 BamHI G'GATC_C Cuts at: 2470 2837 2470 Size: 367 4773 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2461 3916 618 Size: 325 217 627 674 1455 1842 Fragments arranged by size: 1842 1455 674 627 325 217 BanII G_rGCy'C Cuts at: 1391 2459 1391 Size: 1068 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4169 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3331 3365 1903 Size: 34 1394 34 3678 Fragments arranged by size: 3678 1394 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3032 4242 3032 Size: 1210 3930 BclI T'GATC_A Cuts at: 1548 2269 1548 Size: 721 4419 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3751 136 Size: 107 122 71 631 605 2079 1525 Fragments arranged by size: 2079 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5135 5135 Size: 5140 BlpI GC'TnA_GC Cuts at: 2557 2557 Size: 5140 Bme1580I G_kGCm'C Cuts at: 1790 2157 3201 4357 1790 Size: 367 1044 1156 2573 Fragments arranged by size: 2573 1156 1044 367 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5140 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2871 3829 442 Size: 416 53 83 399 24 1454 958 1753 Fragments arranged by size: 1753 1454 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5140 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2342 3800 1239 Size: 1103 1458 2579 Fragments arranged by size: 2579 1458 1103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3265 4130 4428 3265 Size: 865 298 3977 Fragments arranged by size: 3977 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3803 882 Size: 617 2304 2219 Fragments arranged by size: 2304 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2931 493 Size: 711 1727 2702 Fragments arranged by size: 2702 1727 711 BsaBI GATnn'nnATC Cuts at: 5134 5134 Size: 5140 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2947 3329 3964 275 Size: 53 83 186 936 1414 382 635 1451 Fragments arranged by size: 1451 1414 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3573 3971 4245 4392 4803 3573 Size: 398 274 147 411 3910 Fragments arranged by size: 3910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4915 Fragments arranged by size: 4915 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 1186 Size: 1075 4065 BseYI C'CCAG_C Cuts at: 4343 4839 4343 Size: 496 4644 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4567 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2590 3351 4379 1540 Size: 275 186 589 761 1028 2301 Fragments arranged by size: 2301 1028 761 589 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2321 3201 3286 4357 2012 Size: 309 880 85 1071 2795 Fragments arranged by size: 2795 1071 880 309 85 BsmI GAATG_Cn' Cuts at: 2651 2744 2651 Size: 93 5047 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2157 2321 2459 3201 3286 4357 Size: 399 222 145 164 138 742 85 1071 Cuts at: 4357 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 222 164 145 138 85 BspEI T'CCGG_A Cuts at: 4803 4803 Size: 5140 BspHI T'CATG_A Cuts at: 3029 4759 3029 Size: 1730 3410 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2495 844 Size: 1651 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3027 1394 Size: 429 1204 3507 Fragments arranged by size: 3507 1204 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3635 3809 65 Size: 1183 2387 174 1396 Fragments arranged by size: 2387 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3784 3971 1700 Size: 110 1974 187 2869 Fragments arranged by size: 2869 1974 187 110 BsrGI T'GTAC_A Cuts at: 96 2082 96 Size: 1986 3154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5140 BssSI C'ACGA_G Cuts at: 3200 4212 3200 Size: 1012 4128 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5140 BstBI TT'CG_AA Cuts at: 3944 3944 Size: 5140 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5140 BstYI r'GATC_y Cuts at: 1972 2470 2837 3222 3239 4691 4777 4789 5135 Size: 498 367 385 17 1452 86 12 346 Cuts at: 5135 1972 Size: 1977 Fragments arranged by size: 1977 1452 498 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2401 2401 Size: 5140 BtgI C'CryG_G Cuts at: 513 1065 2149 513 Size: 552 1084 3504 Fragments arranged by size: 3504 1084 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4401 Fragments arranged by size: 4401 683 56 BtsI GCAGTG_nn' Cuts at: 2656 3450 3470 2656 Size: 794 20 4326 Fragments arranged by size: 4326 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5140 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5105 DraI TTT'AAA Cuts at: 2444 2444 Size: 5140 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4147 809 Size: 507 2831 1802 Fragments arranged by size: 2831 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2023 2146 2587 Size: 54 1425 50 275 18 193 123 441 Cuts at: 2587 3476 5105 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 441 275 193 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2587 1537 Size: 275 775 4090 Fragments arranged by size: 4090 775 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3070 1353 Size: 1717 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3727 4101 4247 387 Size: 1064 138 2138 374 146 1280 Fragments arranged by size: 2138 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2342 3203 3800 4587 4855 1239 Size: 393 415 295 861 597 787 268 1524 Fragments arranged by size: 1524 861 787 597 415 393 295 268 EcoRI G'AATT_C Cuts at: 2449 2827 2449 Size: 378 4762 FspI TGC'GCA Cuts at: 3646 3646 Size: 5140 HaeII r_GCGC'y Cuts at: 1678 4287 1678 Size: 2609 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3781 3813 3855 3887 1373 Size: 32 135 32 2209 32 42 32 2626 Fragments arranged by size: 2626 2209 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2484 2730 669 Size: 988 281 546 246 3079 Fragments arranged by size: 3079 988 546 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5140 HpaI GTT'AAC Cuts at: 2730 2730 Size: 5140 KpnI G_GTAC'C Cuts at: 2465 2465 Size: 5140 MfeI C'AATT_G Cuts at: 2739 2739 Size: 5140 MluI A'CGCG_T Cuts at: 5016 5016 Size: 5140 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4254 4438 1305 Size: 2949 184 2007 Fragments arranged by size: 2949 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2148 10 Size: 54 1425 343 316 3002 Fragments arranged by size: 3002 1425 343 316 54 MslI CAynn'nnrTG Cuts at: 518 1166 3098 3457 3616 518 Size: 648 1932 359 159 2042 Fragments arranged by size: 2042 1932 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2556 3233 4381 4626 1606 Size: 950 677 1148 245 2120 Fragments arranged by size: 2120 1148 950 677 245 NcoI C'CATG_G Cuts at: 513 1065 2149 513 Size: 552 1084 3504 Fragments arranged by size: 3504 1084 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5140 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5140 NotI GC'GGCC_GC Cuts at: 2587 2587 Size: 5140 NspI r_CATG'y Cuts at: 1132 1229 2498 4043 1132 Size: 97 1269 1545 2229 Fragments arranged by size: 2229 1545 1269 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5140 PciI A'CATG_T Cuts at: 4039 4039 Size: 5140 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5140 PmeI GTTT'AAAC Cuts at: 2444 2444 Size: 5140 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3209 3241 4722 4754 3209 Size: 32 1481 32 3595 Fragments arranged by size: 3595 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5140 PsiI TTA'TAA Cuts at: 2163 2388 2430 2710 2163 Size: 225 42 280 4593 Fragments arranged by size: 4593 280 225 42 PstI C_TGCA'G Cuts at: 830 2492 830 Size: 1662 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5140 SalI G'TCGA_C Cuts at: 1936 2482 1936 Size: 546 4594 SbfI CC_TGCA'GG Cuts at: 2492 2492 Size: 5140 ScaI AGT'ACT Cuts at: 3388 5100 3388 Size: 1712 3428 SfcI C'TryA_G Cuts at: 826 1045 2373 2488 3623 4304 4495 826 Size: 219 1328 115 1135 681 191 1471 Fragments arranged by size: 1471 1328 1135 681 219 191 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5140 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5140 SmaI CCC'GGG Cuts at: 1872 2467 1872 Size: 595 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3244 4145 4407 820 Size: 197 926 1301 901 262 1553 Fragments arranged by size: 1553 1301 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2931 493 Size: 2438 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5140 SphI G_CATG'C Cuts at: 1132 2498 1132 Size: 1366 3774 SspI AAT'ATT Cuts at: 5 52 2419 3064 5 Size: 47 2367 645 2081 Fragments arranged by size: 2367 2081 645 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5140 StyI C'CwwG_G Cuts at: 513 1065 2149 2579 513 Size: 552 1084 430 3074 Fragments arranged by size: 3074 1084 552 430 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3167 3184 3337 3522 3967 325 Size: 1255 1587 17 153 185 445 1498 Fragments arranged by size: 1587 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2082 2179 3386 Size: 274 80 33 51 1443 105 97 1207 Cuts at: 3386 5098 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1207 274 138 105 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5100 5132 5100 Size: 32 5108 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5140 XbaI T'CTAG_A Cuts at: 2476 2476 Size: 5140 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5140 XmaI C'CCGG_G Cuts at: 1870 2465 1870 Size: 595 4545 XmnI GAAnn'nnTTC Cuts at: 3269 3269 Size: 5140 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2948 276 Size: 53 83 186 936 1414 2468 Fragments arranged by size: 2468 1414 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI