(Circular) (Six-base) MAPSORT of: pFN21AB6385.seq Check: 2873 from: 1 to: 5524 pFN21AB6385 5524 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3334 278 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2430 2867 1937 Size: 493 437 4594 Fragments arranged by size: 4594 493 437 Acc65I G'GTAC_C Cuts at: 2388 2845 2388 Size: 457 5067 AclI AA'CG_TT Cuts at: 1563 3651 4024 1563 Size: 2088 373 3063 Fragments arranged by size: 3063 2088 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2030 2368 3587 4971 5239 1632 Size: 398 338 1219 1384 268 1917 Fragments arranged by size: 1917 1384 1219 398 338 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5327 AflIII A'CryG_T Cuts at: 1203 2077 2319 4423 5400 1203 Size: 874 242 2104 977 1327 Fragments arranged by size: 2104 1327 977 874 242 AgeI A'CCGG_T Cuts at: 2541 4355 2541 Size: 1814 3710 AhdI GACnn_n'nnGTC Cuts at: 4253 4253 Size: 5524 AleI CACnn'nnGTG Cuts at: 1166 2403 1166 Size: 1237 4287 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5524 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2067 2099 2067 Size: 32 5492 AlwNI CAG_nnn'CTG Cuts at: 2581 3267 4839 2581 Size: 686 1572 3266 Fragments arranged by size: 3266 1572 686 ApaLI G'TGCA_C Cuts at: 3581 4737 3581 Size: 1156 4368 ApoI r'AATT_y Cuts at: 1493 2833 3059 3211 5320 1493 Size: 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 226 152 AseI AT'TA_AT Cuts at: 160 1032 4078 160 Size: 872 3046 1606 Fragments arranged by size: 3046 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 AvaI C'yCGr_G Cuts at: 1870 1943 2028 2086 2849 1870 Size: 73 85 58 763 4545 Fragments arranged by size: 4545 763 85 73 58 BamHI G'GATC_C Cuts at: 2854 3221 2854 Size: 367 5157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2165 2388 2666 2845 4300 Size: 325 217 627 378 223 278 179 1455 Cuts at: 4300 618 Size: 1842 Fragments arranged by size: 1842 1455 627 378 325 278 223 217 179 BanII G_rGCy'C Cuts at: 1391 2028 2094 2343 2507 2627 2693 2706 2843 Size: 637 66 249 164 120 66 13 137 Cuts at: 2843 1391 Size: 4072 Fragments arranged by size: 4072 637 249 164 137 120 66 66 13 BbeI G_GCGC'C Cuts at: 2169 2670 2169 Size: 501 5023 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2544 928 Size: 971 645 3908 Fragments arranged by size: 3908 971 645 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3715 3749 1903 Size: 34 50 34 1694 34 3678 Fragments arranged by size: 3678 1694 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3416 4626 3416 Size: 1210 4314 BclI T'GATC_A Cuts at: 1548 2652 1548 Size: 1104 4420 BfrBI ATG'CAT Cuts at: 2066 2066 Size: 5524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4135 136 Size: 107 122 71 631 605 2463 1525 Fragments arranged by size: 2463 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5519 5519 Size: 5524 BlpI GC'TnA_GC Cuts at: 2941 2941 Size: 5524 Bme1580I G_kGCm'C Cuts at: 1790 3585 4741 1790 Size: 1795 1156 2573 Fragments arranged by size: 2573 1795 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3255 4213 442 Size: 416 53 83 399 24 1838 958 1753 Fragments arranged by size: 1838 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2280 2585 2804 4184 1239 Size: 803 238 305 219 1380 2579 Fragments arranged by size: 2579 1380 803 305 238 219 Bpu10I CC'TnA_GC Cuts at: 2574 2574 Size: 5524 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2078 3649 4514 4812 2078 Size: 1571 865 298 2790 Fragments arranged by size: 2790 1571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4187 882 Size: 617 2688 2219 Fragments arranged by size: 2688 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2401 3315 493 Size: 711 1197 914 2702 Fragments arranged by size: 2702 1197 914 711 BsaBI GATnn'nnATC Cuts at: 5518 5518 Size: 5524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 2274 2300 2667 Size: 53 83 186 936 633 108 26 367 Cuts at: 2667 3331 3713 4348 275 Size: 664 382 635 1451 Fragments arranged by size: 1451 936 664 635 633 382 367 186 108 83 53 26 BsaWI w'CCGG_w Cuts at: 2541 3957 4355 4629 4776 5187 2541 Size: 1416 398 274 147 411 2878 Fragments arranged by size: 2878 1416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2067 2097 2609 2639 1178 Size: 30 165 30 664 30 512 30 4063 Fragments arranged by size: 4063 664 512 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5524 BseYI C'CCAG_C Cuts at: 4727 5223 4727 Size: 496 5028 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2825 1163 Size: 573 1089 3862 Fragments arranged by size: 3862 1089 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2196 2974 3735 4763 1540 Size: 275 186 195 778 761 1028 2301 Fragments arranged by size: 2301 1028 778 761 275 195 186 BsiHKAI G_wGCw'C Cuts at: 2693 3585 3670 4741 2693 Size: 892 85 1071 3476 Fragments arranged by size: 3476 1071 892 85 BsmI GAATG_Cn' Cuts at: 3035 3128 3035 Size: 93 5431 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2094 2343 2507 2627 2693 2706 Size: 399 238 66 249 164 120 66 13 Cuts at: 2706 2843 3585 3670 4741 1391 Size: 137 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 249 238 164 137 120 85 66 66 13 BspEI T'CCGG_A Cuts at: 5187 5187 Size: 5524 BspHI T'CATG_A Cuts at: 3413 5143 3413 Size: 1730 3794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2879 844 Size: 2035 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3411 1394 Size: 429 1588 3507 Fragments arranged by size: 3507 1588 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2641 4019 4193 65 Size: 1183 1393 1378 174 1396 Fragments arranged by size: 1396 1393 1378 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2541 4168 4355 1700 Size: 110 731 1627 187 2869 Fragments arranged by size: 2869 1627 731 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5524 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5524 BssSI C'ACGA_G Cuts at: 3584 4596 3584 Size: 1012 4512 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5524 BstBI TT'CG_AA Cuts at: 4328 4328 Size: 5524 BstEII G'GTnAC_C Cuts at: 1184 2615 1184 Size: 1431 4093 BstXI CCAn_nnnn'nTGG Cuts at: 2293 2293 Size: 5524 BstYI r'GATC_y Cuts at: 1972 2220 2607 2854 3221 3606 3623 5075 5161 Size: 248 387 247 367 385 17 1452 86 Cuts at: 5161 5173 5519 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 387 385 367 346 248 247 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2268 2352 2467 513 Size: 552 1203 84 115 3570 Fragments arranged by size: 3570 1203 552 115 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2113 2215 2394 487 Size: 683 56 887 102 179 3617 Fragments arranged by size: 3617 887 683 179 102 56 BtsI GCAGTG_nn' Cuts at: 3040 3834 3854 3040 Size: 794 20 4710 Fragments arranged by size: 4710 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5489 DraI TTT'AAA Cuts at: 2828 2828 Size: 5524 DraIII CAC_nnn'GTG Cuts at: 2734 2734 Size: 5524 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4531 809 Size: 507 3215 1802 Fragments arranged by size: 3215 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 2719 2971 Size: 54 1425 50 275 18 519 370 252 Cuts at: 2971 3860 5489 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 519 370 275 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2971 1537 Size: 275 1159 4090 Fragments arranged by size: 4090 1159 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2387 2705 3454 1353 Size: 1034 318 749 3423 Fragments arranged by size: 3423 1034 749 318 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2216 4111 4485 4631 387 Size: 1064 138 627 1895 374 146 1280 Fragments arranged by size: 1895 1280 1064 627 374 146 138 EcoICRI GAG'CTC Cuts at: 2691 2691 Size: 5524 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2042 2280 2368 2585 2804 3587 Size: 393 398 12 238 88 217 219 783 Cuts at: 3587 4184 4971 5239 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 783 597 398 393 268 238 219 217 88 12 EcoO109I rG'GnC_Cy Cuts at: 2098 2098 Size: 5524 EcoRI G'AATT_C Cuts at: 2833 3211 2833 Size: 378 5146 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2242 2242 Size: 5524 FspI TGC'GCA Cuts at: 4030 4030 Size: 5524 HaeII r_GCGC'y Cuts at: 1678 2169 2670 4671 1678 Size: 491 501 2001 2531 Fragments arranged by size: 2531 2001 501 491 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2433 2465 2609 2641 4165 Size: 32 135 32 861 32 144 32 1524 Cuts at: 4165 4197 4239 4271 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1524 861 144 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2868 3114 669 Size: 988 281 930 246 3079 Fragments arranged by size: 3079 988 930 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5524 HpaI GTT'AAC Cuts at: 3114 3114 Size: 5524 KasI G'GCGC_C Cuts at: 2165 2666 2165 Size: 501 5023 KpnI G_GTAC'C Cuts at: 2392 2849 2392 Size: 457 5067 MfeI C'AATT_G Cuts at: 3123 3123 Size: 5524 MluI A'CGCG_T Cuts at: 2077 2319 5400 2077 Size: 242 3081 2201 Fragments arranged by size: 3081 2201 242 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4638 4822 1305 Size: 3333 184 2007 Fragments arranged by size: 3333 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2351 10 Size: 54 1425 343 519 3183 Fragments arranged by size: 3183 1425 519 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2291 2403 2466 3482 3841 4000 518 Size: 648 1125 112 63 1016 359 159 2042 Fragments arranged by size: 2042 1125 1016 648 359 159 112 63 MspA1I CmG'CkG Cuts at: 1606 2270 2750 2940 3617 4765 5010 1606 Size: 664 480 190 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 480 245 190 NarI GG'CG_CC Cuts at: 2166 2667 2166 Size: 501 5023 NcoI C'CATG_G Cuts at: 513 1065 2467 513 Size: 552 1402 3570 Fragments arranged by size: 3570 1402 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5524 NotI GC'GGCC_GC Cuts at: 2971 2971 Size: 5524 NsiI A_TGCA'T Cuts at: 2068 2068 Size: 5524 NspI r_CATG'y Cuts at: 1132 1229 2649 2882 4427 1132 Size: 97 1420 233 1545 2229 Fragments arranged by size: 2229 1545 1420 233 97 PasI CC'CwG_GG Cuts at: 1348 2500 1348 Size: 1152 4372 PciI A'CATG_T Cuts at: 4423 4423 Size: 5524 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5524 PmeI GTTT'AAAC Cuts at: 2828 2828 Size: 5524 PmlI CAC'GTG Cuts at: 2401 2401 Size: 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2067 2099 3593 3625 5106 5138 2067 Size: 32 1494 32 1481 32 2453 Fragments arranged by size: 2453 1494 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2098 2098 Size: 5524 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5524 PsiI TTA'TAA Cuts at: 3094 3094 Size: 5524 PstI C_TGCA'G Cuts at: 830 2876 830 Size: 2046 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5524 PvuII CAG'CTG Cuts at: 2750 2750 Size: 5524 SacI G_AGCT'C Cuts at: 2693 2693 Size: 5524 SacII CC_GC'GG Cuts at: 2271 2271 Size: 5524 SalI G'TCGA_C Cuts at: 1936 2866 1936 Size: 930 4594 SapI GCTCTTCn'nnn_ Cuts at: 2387 2387 Size: 5524 SbfI CC_TGCA'GG Cuts at: 2876 2876 Size: 5524 ScaI AGT'ACT Cuts at: 3772 5484 3772 Size: 1712 3812 SfcI C'TryA_G Cuts at: 826 1045 2476 2872 4007 4688 4879 826 Size: 219 1431 396 1135 681 191 1471 Fragments arranged by size: 1471 1431 1135 681 396 219 191 SfoI GGC'GCC Cuts at: 2167 2668 2167 Size: 501 5023 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5524 SmaI CCC'GGG Cuts at: 1872 2088 2851 1872 Size: 216 763 4545 Fragments arranged by size: 4545 763 216 SmlI C'TyrA_G Cuts at: 820 1017 1943 2093 3628 4529 4791 820 Size: 197 926 150 1535 901 262 1553 Fragments arranged by size: 1553 1535 926 901 262 197 150 SnaBI TAC'GTA Cuts at: 493 3315 493 Size: 2822 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5524 SphI G_CATG'C Cuts at: 1132 2882 1132 Size: 1750 3774 SspI AAT'ATT Cuts at: 5 52 3448 5 Size: 47 3396 2081 Fragments arranged by size: 3396 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5524 StyI C'CwwG_G Cuts at: 513 1065 2467 2963 513 Size: 552 1402 496 3074 Fragments arranged by size: 3074 1402 552 496 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2182 2393 2603 2710 3551 3568 3721 Size: 1255 602 211 210 107 841 17 153 Cuts at: 3721 3906 4351 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 841 602 445 211 210 185 153 107 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3770 5482 96 Size: 274 80 33 51 1443 1793 1712 138 Fragments arranged by size: 1793 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2599 2631 5484 5516 2599 Size: 32 2853 32 2607 Fragments arranged by size: 2853 2607 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2153 1316 Size: 837 4687 XbaI T'CTAG_A Cuts at: 2860 2860 Size: 5524 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2134 2293 2735 2134 Size: 159 442 4923 Fragments arranged by size: 4923 442 159 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5524 XmaI C'CCGG_G Cuts at: 1870 2086 2849 1870 Size: 216 763 4545 Fragments arranged by size: 4545 763 216 XmnI GAAnn'nnTTC Cuts at: 3653 3653 Size: 5524 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3332 276 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI