Restriction map for
pFN21AB6515
(Linear) (Six-base) MAPSORT of: KIBB6515.seq Check: 8481 from: 1 to: 1080
KIBB6515.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 625 1080
Size: 418 207 455
Fragments arranged by size:
455 418 207
AflII C'TTAA_G
Cuts at: 0 996 1080
Size: 996 84
AflIII A'CryG_T
Cuts at: 0 442 1080
Size: 442 638
AleI CACnn'nnGTG
Cuts at: 0 452 1080
Size: 452 628
AlwNI CAG_nnn'CTG
Cuts at: 0 167 1080
Size: 167 913
AvaI C'yCGr_G
Cuts at: 0 836 1080
Size: 836 244
BanI G'GyrC_C
Cuts at: 0 257 1080
Size: 257 823
BanII G_rGCy'C
Cuts at: 0 922 1080
Size: 922 158
BbsI GAAGACnn'nnnn_
Cuts at: 0 27 406 721 1080
Size: 27 379 315 359
Fragments arranged by size:
379 359 315 27
BclI T'GATC_A
Cuts at: 0 488 1080
Size: 488 592
BglII A'GATC_T
Cuts at: 0 690 1080
Size: 690 390
BmrI ACTGGGnnnn_n'
Cuts at: 0 617 1080
Size: 617 463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 518 962 1080
Size: 370 148 444 118
Fragments arranged by size:
444 370 148 118
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 87 117 909 939 1080
Size: 87 30 792 30 141
Fragments arranged by size:
792 141 87 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 92 95 545 548 551 575 930 1080
Size: 92 3 450 3 3 24 355 150
Fragments arranged by size:
450 355 150 92 24 3 3 3
BsmBI CGTCTCn'nnnn_
Cuts at: 0 789 1080
Size: 789 291
Bsp1286I G_dGCh'C
Cuts at: 0 922 1080
Size: 922 158
BstYI r'GATC_y
Cuts at: 0 690 879 1080
Size: 690 189 201
Fragments arranged by size:
690 201 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 737 1080
Size: 737 343
BtsI GCAGTG_nn'
Cuts at: 0 526 1080
Size: 526 554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 418 518 625 962 1080
Size: 370 48 100 107 337 118
Fragments arranged by size:
370 337 118 107 100 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 532 1080
Size: 532 548
HindIII A'AGCT_T
Cuts at: 0 742 1080
Size: 742 338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1080
Size: 1063 17
MslI CAynn'nnrTG
Cuts at: 0 452 1080
Size: 452 628
MspA1I CmG'CkG
Cuts at: 0 254 1014 1080
Size: 254 760 66
Fragments arranged by size:
760 254 66
NspI r_CATG'y
Cuts at: 0 446 802 1080
Size: 446 356 278
Fragments arranged by size:
446 356 278
PciI A'CATG_T
Cuts at: 0 442 1080
Size: 442 638
PflMI CCAn_nnn'nTGG
Cuts at: 0 433 1080
Size: 433 647
PfoI T'CCnGG_A
Cuts at: 0 181 386 861 1080
Size: 181 205 475 219
Fragments arranged by size:
475 219 205 181
PspXI vC'TCGA_Gb
Cuts at: 0 836 1080
Size: 836 244
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 921 953 1080
Size: 921 32 127
Fragments arranged by size:
921 127 32
PvuII CAG'CTG
Cuts at: 0 254 1014 1080
Size: 254 760 66
Fragments arranged by size:
760 254 66
SmlI C'TyrA_G
Cuts at: 0 836 996 1080
Size: 836 160 84
Fragments arranged by size:
836 160 84
SpeI A'CTAG_T
Cuts at: 0 774 1080
Size: 774 306
StuI AGG'CCT
Cuts at: 0 994 1080
Size: 994 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 67 99 291 323 909 941 1080
Size: 67 32 192 32 586 32 139
Fragments arranged by size:
586 192 139 67 32 32 32
XhoI C'TCGA_G
Cuts at: 0 836 1080
Size: 836 244
Enzymes that do cut:
AcuI AflII AflIII AleI AlwNI AvaI BanI BanII
BbsI BclI BglII BmrI BpmI BsaXI BseRI BsmBI
Bsp1286I BstYI BtgZI BtsI Eco57MI FalI HindIII MmeI
MslI MspA1I NspI PciI PflMI PfoI PspXI PsrI
PvuII SmlI SpeI StuI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI StyI SwaI TaqII TatI
Tth111I XbaI XcmI XmaI XmnI ZraI