(Circular) (Six-base) MAPSORT of: pFN21AB6582.seq Check: 5915 from: 1 to: 5917 pFN21AB6582 5917 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3260 1937 Size: 1323 4594 Acc65I G'GTAC_C Cuts at: 3238 3238 Size: 5917 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2378 3980 5364 5632 1632 Size: 746 1602 1384 268 1917 Fragments arranged by size: 1917 1602 1384 746 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 4816 5793 1203 Size: 3613 977 1327 Fragments arranged by size: 3613 1327 977 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 2239 4646 2239 Size: 2407 3510 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5917 AlwNI CAG_nnn'CTG Cuts at: 2179 3660 5232 2179 Size: 1481 1572 2864 Fragments arranged by size: 2864 1572 1481 ApaI G_GGCC'C Cuts at: 3123 3123 Size: 5917 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 2096 3226 3452 3604 5713 1493 Size: 603 1130 226 152 2109 1697 Fragments arranged by size: 2109 1697 1130 603 226 152 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 2650 3209 3242 1870 Size: 73 707 559 33 4545 Fragments arranged by size: 4545 707 559 73 33 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3238 4693 618 Size: 325 217 627 1451 1455 1842 Fragments arranged by size: 1842 1455 1451 627 325 217 BanII G_rGCy'C Cuts at: 1391 2034 3123 3236 1391 Size: 643 1089 113 4072 Fragments arranged by size: 4072 1089 643 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4946 BbvCI CC'TCA_GC Cuts at: 3052 3052 Size: 5917 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3809 5019 3809 Size: 1210 4707 BclI T'GATC_A Cuts at: 1548 2434 1548 Size: 886 5031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4528 136 Size: 107 122 71 631 605 2856 1525 Fragments arranged by size: 2856 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2844 5912 2844 Size: 3068 2849 BlpI GC'TnA_GC Cuts at: 3334 3334 Size: 5917 Bme1580I G_kGCm'C Cuts at: 1790 2784 3123 3978 5134 1790 Size: 994 339 855 1156 2573 Fragments arranged by size: 2573 1156 994 855 339 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3648 4606 442 Size: 416 53 83 399 24 2231 958 1753 Fragments arranged by size: 2231 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3172 4577 1239 Size: 1933 1405 2579 Fragments arranged by size: 2579 1933 1405 Bpu10I CC'TnA_GC Cuts at: 3052 3052 Size: 5917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2436 4042 4907 5205 2436 Size: 1606 865 298 3148 Fragments arranged by size: 3148 1606 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2306 4580 882 Size: 617 807 2274 2219 Fragments arranged by size: 2274 2219 807 617 BsaAI yAC'GTr Cuts at: 493 1204 3708 493 Size: 711 2504 2702 Fragments arranged by size: 2702 2504 711 BsaBI GATnn'nnATC Cuts at: 2346 5911 2346 Size: 3565 2352 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2223 3724 4106 4741 Size: 53 83 186 936 690 1501 382 635 Cuts at: 4741 275 Size: 1451 Fragments arranged by size: 1501 1451 936 690 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4350 4748 5022 5169 5580 4350 Size: 398 274 147 411 4687 Fragments arranged by size: 4687 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5692 Fragments arranged by size: 5692 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 1186 Size: 1075 4842 BseYI C'CCAG_C Cuts at: 2140 5120 5616 2140 Size: 2980 496 2441 Fragments arranged by size: 2980 2441 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3367 4128 5156 1540 Size: 275 186 1366 761 1028 2301 Fragments arranged by size: 2301 1366 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3978 4063 5134 3978 Size: 85 1071 4761 Fragments arranged by size: 4761 1071 85 BsmI GAATG_Cn' Cuts at: 3101 3428 3521 3101 Size: 327 93 5497 Fragments arranged by size: 5497 327 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2784 3123 3236 3978 4063 5134 Size: 399 244 750 339 113 742 85 1071 Cuts at: 5134 1391 Size: 2174 Fragments arranged by size: 2174 1071 750 742 399 339 244 113 85 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3135 3806 5536 3135 Size: 671 1730 3516 Fragments arranged by size: 3516 1730 671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3205 3272 844 Size: 2361 67 3489 Fragments arranged by size: 3489 2361 67 BsrBI CCG'CTC Cuts at: 1394 1823 2589 3804 1394 Size: 429 766 1215 3507 Fragments arranged by size: 3507 1215 766 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4412 4586 65 Size: 1183 3164 174 1396 Fragments arranged by size: 3164 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4561 4748 1700 Size: 110 2751 187 2869 Fragments arranged by size: 2869 2751 187 110 BsrGI T'GTAC_A Cuts at: 96 2640 3094 96 Size: 2544 454 2919 Fragments arranged by size: 2919 2544 454 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5917 BstBI TT'CG_AA Cuts at: 4721 4721 Size: 5917 BstEII G'GTnAC_C Cuts at: 1184 3028 1184 Size: 1844 4073 BstYI r'GATC_y Cuts at: 1972 2019 2347 2844 3196 3247 3614 3999 4016 Size: 47 328 497 352 51 367 385 17 Cuts at: 4016 5468 5554 5566 5912 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 497 385 367 352 346 328 86 51 47 17 12 BtgI C'CryG_G Cuts at: 513 1065 2611 513 Size: 552 1546 3819 Fragments arranged by size: 3819 1546 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5178 Fragments arranged by size: 5178 683 56 BtsI GCAGTG_nn' Cuts at: 2109 3433 4227 4247 2109 Size: 1324 794 20 3779 Fragments arranged by size: 3779 1324 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2944 2979 529 Size: 35 2380 35 3467 Fragments arranged by size: 3467 2380 35 35 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4924 809 Size: 507 3608 1802 Fragments arranged by size: 3608 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3364 4253 5882 Size: 54 1425 50 275 18 1534 889 1629 Cuts at: 5882 8 Size: 43 Fragments arranged by size: 1629 1534 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 1537 Size: 275 1552 4090 Fragments arranged by size: 4090 1552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2328 2741 3134 3847 1353 Size: 975 413 393 713 3423 Fragments arranged by size: 3423 975 713 413 393 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4504 4878 5024 387 Size: 1064 138 2915 374 146 1280 Fragments arranged by size: 2915 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2378 3172 3980 4577 5364 5632 1239 Size: 393 746 794 808 597 787 268 1524 Fragments arranged by size: 1524 808 794 787 746 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2126 3212 2126 Size: 1086 4831 EcoO109I rG'GnC_Cy Cuts at: 3119 3120 3119 Size: 1 5916 EcoRI G'AATT_C Cuts at: 2096 3226 3604 2096 Size: 1130 378 4409 Fragments arranged by size: 4409 1130 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2705 2705 Size: 5917 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 5064 1678 Size: 3386 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2286 2318 2829 Size: 32 135 32 653 32 29 32 511 Cuts at: 2829 2861 4558 4590 4632 4664 1373 Size: 32 1697 32 42 32 2626 Fragments arranged by size: 2626 1697 653 511 135 42 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 3261 3507 669 Size: 988 281 1323 246 3079 Fragments arranged by size: 3079 1323 988 281 246 HindIII A'AGCT_T Cuts at: 748 2400 748 Size: 1652 4265 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KpnI G_GTAC'C Cuts at: 3242 3242 Size: 5917 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2223 3046 5031 5215 1305 Size: 918 823 1985 184 2007 Fragments arranged by size: 2007 1985 918 823 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4095 Fragments arranged by size: 4095 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3875 4234 4393 518 Size: 648 2709 359 159 2042 Fragments arranged by size: 2709 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2991 3169 3333 4010 5158 5403 1606 Size: 1385 178 164 677 1148 245 2120 Fragments arranged by size: 2120 1385 1148 677 245 178 164 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5365 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 2501 3275 4820 1132 Size: 97 1272 774 1545 2229 Fragments arranged by size: 2229 1545 1272 774 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 2179 1108 Size: 1071 4846 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5917 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PspOMI G'GGCC_C Cuts at: 3119 3119 Size: 5917 PstI C_TGCA'G Cuts at: 830 2049 3269 830 Size: 1219 1220 3478 Fragments arranged by size: 3478 1220 1219 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 PvuII CAG'CTG Cuts at: 3169 3169 Size: 5917 RsrII CG'GwC_CG Cuts at: 2209 2209 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 2111 3008 4165 5877 2111 Size: 897 1157 1712 2151 Fragments arranged by size: 2151 1712 1157 897 SfcI C'TryA_G Cuts at: 826 1045 2045 3265 4400 5081 5272 826 Size: 219 1000 1220 1135 681 191 1471 Fragments arranged by size: 1471 1220 1135 1000 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 3244 1872 Size: 1372 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2451 4021 4922 5184 820 Size: 197 926 508 1570 901 262 1553 Fragments arranged by size: 1570 1553 926 901 508 262 197 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5917 StyI C'CwwG_G Cuts at: 513 1065 2351 2509 3356 513 Size: 552 1286 158 847 3074 Fragments arranged by size: 3074 1286 847 552 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3944 3961 4114 4299 4744 325 Size: 1255 2364 17 153 185 445 1498 Fragments arranged by size: 2364 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2109 2640 3006 Size: 274 80 33 51 1443 132 531 366 Cuts at: 3006 3094 4163 5875 96 Size: 88 1069 1712 138 Fragments arranged by size: 1712 1443 1069 531 366 274 138 132 88 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5877 5909 5877 Size: 32 5885 Tth111I GACn'n_nGTC Cuts at: 1316 2090 2213 1316 Size: 774 123 5020 Fragments arranged by size: 5020 774 123 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2516 3196 2516 Size: 680 5237 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 3242 1870 Size: 1372 4545 XmnI GAAnn'nnTTC Cuts at: 4046 4046 Size: 5917 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI