Restriction map for
pFN21AB7860
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BfrI BglI BglII BlnI
BsaBI BsaHI BsiEI BsiWI BsmBI
BspDI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Alw44I 1762 GTGCAC G^TGCAC
AlwI 775 GATCC GGATC(4/5)
ApaLI 1762 GTGCAC G^TGCAC
Asp700I 615 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 44 CCCGGG C^[CT]CG[GA]G
BalI 1389 TGGCCA TGG^CCA
BanI 261 GGCACC G^G[CT][GA]CC
BanII 64 GGGCTC G[GA]GC[CT]^C
BbvCI 388 GCTGAGG CCTCAGC(-5/-2)
BciVI 1239 GGATAC GTATCC(6/5)
BfaI 446 CTAG C^TAG
BlpI 547 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 547 GCTGAGC GC^T[ACGT]AGC
BsaAI 13 TACGTG [CT]AC^GT[GA]
BsaI 1110 GAGACC GGTCTC(1/5)
BsgI 1554 GTGCAG GTGCAG(16/14)
BsoBI 44 CCCGGG C^[CT]CG[GA]G
Bsp1407I 203 TGTACA T^GTACA
BspMI 1172 ACCTGC ACCTGC(4/8)
BsrDI 563 CATTGC GCAATG(2/0)
BsrGI 203 TGTACA T^GTACA
BstAPI 633 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 774 AGATCC [GA]^GATC[CT]
CelII 547 GCTGAGC GC^T[ACGT]AGC
EaeI 1389 TGGCCA [CT]^GGCC[GA]
EcoNI 1226 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 787 AAGCTT A^AGCTT
MaeI 446 CTAG C^TAG
MflI 774 AGATCC [GA]^GATC[CT]
MluNI 1389 TGGCCA TGG^CCA
MscI 1389 TGGCCA TGG^CCA
NcoI 1779 CCATGG C^CATGG
NdeI 919 CATATG CA^TATG
NspI 8 GCATGT [GA]CATG^[CT]
PflMI 1381 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1686 GACTC GAGTC(4/5)
PvuII 708 CAGCTG CAG^CTG
SapI 726 GAAGAGC GCTCTTC(1/4)
SfcI 835 CTACAG C^T[GA][CT]AG
SmaI 44 CCCGGG CCC^GGG
SspBI 203 TGTACA T^GTACA
Tsp45I 377 GTGAC ^GT[GC]AC
Van91I 1381 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 445 TCTAGA T^CTAGA
XcmI 1697 CCAATCGTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 774 AGATCC [GA]^GATC[CT]
XmaCI 44 CCCGGG C^CCGGG
XmaI 44 CCCGGG C^CCGGG
XmnI 615 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccIII 1145 TCCGGA T^CCGGA
1430 TCCGGA
AciI 78 CCGC CCGC(-3/-1)
463 GCGG
AvaII 106 GGACC G^G[AT]CC
1148 GGACC
BclI 923 TGATCA T^GATCA
987 TGATCA
BsaWI 1145 TCCGGA [AT]^CCGG[AT]
1430 TCCGGA
BseAI 1145 TCCGGA T^CCGGA
1430 TCCGGA
BsmFI 1451 GTCCC GGGAC(10/14)
1703 GTCCC
BsmI 128 GAATGC GAATGC(1/-1)
1714 GAATGC
BspEI 1145 TCCGGA T^CCGGA
1430 TCCGGA
DsaI 226 CCACGG C^C[GA][CT]GG
1779 CCATGG
EarI 123 GAAGAG CTCTTC(1/4)
726 GAAGAG
EcoT14I 480 CCTTGG C^C[AT][AT]GG
1779 CCATGG
FauI 77 CCCGC CCCGC(4/6)
463 GCGGG
FbaI 923 TGATCA T^GATCA
987 TGATCA
HgaI 1602 GACGC GACGC(5/10)
1449 GCGTC
Ksp632I 123 GAAGAG CTCTTC(1/4)
726 GAAGAG
MaeII 14 ACGT A^CGT
915 ACGT
MaeIII 377 GTGAC ^GT[ACGT]AC
1318 GTTAC
MroI 1145 TCCGGA T^CCGGA
1430 TCCGGA
MspA1I 461 CAGCGG C[AC]G^C[GT]G
708 CAGCTG
StyI 480 CCTTGG C^C[AT][AT]GG
1779 CCATGG
TaqI 520 TCGA T^CGA
1674 TCGA
TthHB8I 520 TCGA T^CGA
1674 TCGA
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI StuI
TfiI Tru9I TseI Tsp509I TspRI