(Circular) (Six-base) MAPSORT of: pFN21AB7860.seq Check: 2067 from: 1 to: 6502 pFN21AB7860 6502 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3186 3186 Size: 6502 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4312 278 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3845 1937 Size: 1908 4594 Acc65I G'GTAC_C Cuts at: 3823 3823 Size: 6502 AclI AA'CG_TT Cuts at: 1563 4629 5002 1563 Size: 3066 373 3063 Fragments arranged by size: 3066 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2060 2160 2750 2811 3315 3527 3556 3683 Size: 428 100 590 61 504 212 29 127 Cuts at: 3683 4565 5949 6217 1632 Size: 882 1384 268 1917 Fragments arranged by size: 1917 1384 882 590 504 428 268 212 127 100 61 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6305 AflIII A'CryG_T Cuts at: 1203 5401 6378 1203 Size: 4198 977 1327 Fragments arranged by size: 4198 1327 977 AgeI A'CCGG_T Cuts at: 5333 5333 Size: 6502 AhdI GACnn_n'nnGTC Cuts at: 5231 5231 Size: 6502 AleI CACnn'nnGTG Cuts at: 1166 3447 1166 Size: 2281 4221 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2036 1184 Size: 852 5650 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3104 3136 3104 Size: 32 6470 AlwNI CAG_nnn'CTG Cuts at: 4245 5817 4245 Size: 1572 4930 ApaLI G'TGCA_C Cuts at: 3767 4559 5715 3767 Size: 792 1156 4554 Fragments arranged by size: 4554 1156 792 ApoI r'AATT_y Cuts at: 1493 2446 2597 2624 2894 3401 3811 4037 4189 Size: 953 151 27 270 507 410 226 152 Cuts at: 4189 6298 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 953 507 410 270 226 152 151 27 AseI AT'TA_AT Cuts at: 160 1032 5056 160 Size: 872 4024 1606 Fragments arranged by size: 4024 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 AvaI C'yCGr_G Cuts at: 1870 1943 2049 3827 1870 Size: 73 106 1778 4545 Fragments arranged by size: 4545 1778 106 73 BamHI G'GATC_C Cuts at: 3832 4199 3832 Size: 367 6135 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 3823 5278 618 Size: 325 217 627 479 1557 1455 1842 Fragments arranged by size: 1842 1557 1455 627 479 325 217 BanII G_rGCy'C Cuts at: 1391 2073 3821 1391 Size: 682 1748 4072 Fragments arranged by size: 4072 1748 682 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2148 2461 3033 3104 928 Size: 971 249 313 572 71 4326 Fragments arranged by size: 4326 971 572 313 249 71 BbvCI CC'TCA_GC Cuts at: 2394 2394 Size: 6502 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4693 4727 1903 Size: 34 50 34 2672 34 3678 Fragments arranged by size: 3678 2672 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3238 4394 5604 3238 Size: 1156 1210 4136 Fragments arranged by size: 4136 1210 1156 BclI T'GATC_A Cuts at: 1548 2928 2992 1548 Size: 1380 64 5058 Fragments arranged by size: 5058 1380 64 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5113 136 Size: 107 122 71 631 605 3441 1525 Fragments arranged by size: 3441 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6497 6497 Size: 6502 BlpI GC'TnA_GC Cuts at: 2553 3919 2553 Size: 1366 5136 Bme1580I G_kGCm'C Cuts at: 1790 3771 4563 5719 1790 Size: 1981 792 1156 2573 Fragments arranged by size: 2573 1981 1156 792 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6502 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2624 4233 5191 Size: 416 53 83 399 24 1207 1609 958 Cuts at: 5191 442 Size: 1753 Fragments arranged by size: 1753 1609 1207 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6502 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2546 2796 3636 5162 1239 Size: 903 404 250 840 1526 2579 Fragments arranged by size: 2579 1526 903 840 404 250 Bpu10I CC'TnA_GC Cuts at: 2394 2394 Size: 6502 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4627 5492 5790 4627 Size: 865 298 5339 Fragments arranged by size: 5339 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3109 5165 882 Size: 617 1610 2056 2219 Fragments arranged by size: 2219 2056 1610 617 BsaAI yAC'GTr Cuts at: 493 1204 2020 4293 493 Size: 711 816 2273 2702 Fragments arranged by size: 2702 2273 816 711 BsaBI GATnn'nnATC Cuts at: 6496 6496 Size: 6502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4309 4691 5326 275 Size: 53 83 186 936 2776 382 635 1451 Fragments arranged by size: 2776 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3150 3435 4935 5333 5607 5754 6165 3150 Size: 285 1500 398 274 147 411 3487 Fragments arranged by size: 3487 1500 411 398 285 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2018 2048 3104 3134 3527 Size: 30 165 30 615 30 1056 30 393 Cuts at: 3527 3557 1178 Size: 30 4123 Fragments arranged by size: 4123 1056 615 393 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2411 2603 3125 1186 Size: 1225 192 522 4563 Fragments arranged by size: 4563 1225 522 192 BseYI C'CCAG_C Cuts at: 2008 5705 6201 2008 Size: 3697 496 2309 Fragments arranged by size: 3697 2309 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3580 1163 Size: 573 1844 4085 Fragments arranged by size: 4085 1844 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3952 4713 5741 1540 Size: 275 186 1951 761 1028 2301 Fragments arranged by size: 2301 1951 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3007 3554 3600 3771 4563 4648 5719 3007 Size: 547 46 171 792 85 1071 3790 Fragments arranged by size: 3790 1071 792 547 171 85 46 BsmI GAATG_Cn' Cuts at: 2139 3725 4013 4106 2139 Size: 1586 288 93 4535 Fragments arranged by size: 4535 1586 288 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 3007 3554 3600 3771 3821 4563 Size: 399 283 934 547 46 171 50 742 Cuts at: 4563 4648 5719 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 934 742 547 399 283 171 85 50 46 BspEI T'CCGG_A Cuts at: 3150 3435 6165 3150 Size: 285 2730 3487 Fragments arranged by size: 3487 2730 285 BspHI T'CATG_A Cuts at: 2260 2860 3274 4391 6121 2260 Size: 600 414 1117 1730 2641 Fragments arranged by size: 2641 1730 1117 600 414 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3186 3857 844 Size: 2342 671 3489 Fragments arranged by size: 3489 2342 671 BsrBI CCG'CTC Cuts at: 1394 1823 4389 1394 Size: 429 2566 3507 Fragments arranged by size: 3507 2566 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2567 4997 5171 65 Size: 1183 1319 2430 174 1396 Fragments arranged by size: 2430 1396 1319 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5146 5333 1700 Size: 110 3336 187 2869 Fragments arranged by size: 3336 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2208 96 Size: 2112 4390 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6502 BssSI C'ACGA_G Cuts at: 4562 5574 4562 Size: 1012 5490 BstAPI GCAn_nnn'nTGC Cuts at: 794 2644 794 Size: 1850 4652 BstBI TT'CG_AA Cuts at: 5306 5306 Size: 6502 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6502 BstYI r'GATC_y Cuts at: 1972 2779 3832 4199 4584 4601 6053 6139 6151 Size: 807 1053 367 385 17 1452 86 12 Cuts at: 6151 6497 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1053 807 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2231 3784 513 Size: 552 1166 1553 3231 Fragments arranged by size: 3231 1553 1166 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3237 487 Size: 683 56 2011 3752 Fragments arranged by size: 3752 2011 683 56 BtsI GCAGTG_nn' Cuts at: 3217 3418 4018 4812 4832 3217 Size: 201 600 794 20 4887 Fragments arranged by size: 4887 794 600 201 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6502 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2596 2631 3034 3069 529 Size: 35 2032 35 403 35 3962 Fragments arranged by size: 3962 2032 403 35 35 35 DraI TTT'AAA Cuts at: 3806 3806 Size: 6502 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5509 809 Size: 507 4193 1802 Fragments arranged by size: 4193 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3394 3949 4838 Size: 54 1425 50 275 18 1564 555 889 Cuts at: 4838 6467 8 Size: 1629 43 Fragments arranged by size: 1629 1564 1425 889 555 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3949 1537 Size: 275 2137 4090 Fragments arranged by size: 4090 2137 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2123 2726 4432 1353 Size: 770 603 1706 3423 Fragments arranged by size: 3423 1706 770 603 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5089 5463 5609 387 Size: 1064 138 3500 374 146 1280 Fragments arranged by size: 3500 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2142 2160 2546 2750 2796 2811 Size: 393 428 82 18 386 204 46 15 Cuts at: 2811 3315 3527 3556 3636 3683 4565 5162 5949 Size: 504 212 29 80 47 882 597 787 Cuts at: 5949 6217 1239 Size: 268 1524 Fragments arranged by size: 1524 882 787 597 504 428 393 386 268 212 204 82 80 47 46 29 18 15 EcoNI CCTnn'n_nnAGG Cuts at: 3235 3235 Size: 6502 EcoRI G'AATT_C Cuts at: 3811 4189 3811 Size: 378 6124 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2501 2501 Size: 6502 FspI TGC'GCA Cuts at: 5008 5008 Size: 6502 HaeII r_GCGC'y Cuts at: 1678 5649 1678 Size: 3971 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 2255 2287 2977 Size: 32 135 32 446 32 205 32 690 Cuts at: 2977 3009 3417 3449 3682 3714 5143 5175 5217 Size: 32 408 32 233 32 1429 32 42 Cuts at: 5217 5249 1373 Size: 32 2626 Fragments arranged by size: 2626 1429 690 446 408 233 205 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3846 4092 669 Size: 988 281 1908 246 3079 Fragments arranged by size: 3079 1908 988 281 246 HindIII A'AGCT_T Cuts at: 748 2792 748 Size: 2044 4458 HpaI GTT'AAC Cuts at: 4092 4092 Size: 6502 KpnI G_GTAC'C Cuts at: 3827 3827 Size: 6502 MfeI C'AATT_G Cuts at: 4101 4101 Size: 6502 MluI A'CGCG_T Cuts at: 6378 6378 Size: 6502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 5616 5800 1305 Size: 960 3351 184 2007 Fragments arranged by size: 3351 2007 960 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3396 10 Size: 54 1425 343 1564 3116 Fragments arranged by size: 3116 1564 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2018 3075 3192 3222 3447 4460 4819 Size: 648 852 1057 117 30 225 1013 359 Cuts at: 4819 4978 518 Size: 159 2042 Fragments arranged by size: 2042 1057 1013 852 648 359 225 159 117 30 MspA1I CmG'CkG Cuts at: 1606 2468 2715 3918 4595 5743 5988 1606 Size: 862 247 1203 677 1148 245 2120 Fragments arranged by size: 2120 1203 1148 862 677 247 245 NcoI C'CATG_G Cuts at: 513 1065 3784 513 Size: 552 2719 3231 Fragments arranged by size: 3231 2719 552 NdeI CA'TA_TG Cuts at: 387 2925 387 Size: 2538 3964 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6502 NotI GC'GGCC_GC Cuts at: 3949 3949 Size: 6502 NspI r_CATG'y Cuts at: 1132 1229 2017 3860 5405 1132 Size: 97 788 1843 1545 2229 Fragments arranged by size: 2229 1843 1545 788 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6502 PciI A'CATG_T Cuts at: 5401 5401 Size: 6502 PflMI CCAn_nnn'nTGG Cuts at: 1108 3392 1108 Size: 2284 4218 PmeI GTTT'AAAC Cuts at: 3806 3806 Size: 6502 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3104 3136 4571 4603 6084 6116 3104 Size: 32 1435 32 1481 32 3490 Fragments arranged by size: 3490 1481 1435 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6502 PsiI TTA'TAA Cuts at: 4072 4072 Size: 6502 PstI C_TGCA'G Cuts at: 830 3854 830 Size: 3024 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6502 PvuII CAG'CTG Cuts at: 2715 2715 Size: 6502 SalI G'TCGA_C Cuts at: 1936 3844 1936 Size: 1908 4594 SapI GCTCTTCn'nnn_ Cuts at: 2726 2726 Size: 6502 SbfI CC_TGCA'GG Cuts at: 3854 3854 Size: 6502 ScaI AGT'ACT Cuts at: 4750 6462 4750 Size: 1712 4790 SfcI C'TryA_G Cuts at: 826 1045 2840 3850 4985 5666 5857 826 Size: 219 1795 1010 1135 681 191 1471 Fragments arranged by size: 1795 1471 1135 1010 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6502 SmaI CCC'GGG Cuts at: 1872 2051 3829 1872 Size: 179 1778 4545 Fragments arranged by size: 4545 1778 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 4606 5507 5769 820 Size: 197 926 2663 901 262 1553 Fragments arranged by size: 2663 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4293 493 Size: 3800 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6502 SphI G_CATG'C Cuts at: 1132 3860 1132 Size: 2728 3774 SspI AAT'ATT Cuts at: 5 52 4426 5 Size: 47 4374 2081 Fragments arranged by size: 4374 2081 47 StuI AGG'CCT Cuts at: 1517 3317 3569 3588 1517 Size: 1800 252 19 4431 Fragments arranged by size: 4431 1800 252 19 StyI C'CwwG_G Cuts at: 513 1065 2485 3784 3941 513 Size: 552 1420 1299 157 3074 Fragments arranged by size: 3074 1420 1299 552 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2948 2990 4529 4546 4699 4884 5329 Size: 1255 1368 42 1539 17 153 185 445 Cuts at: 5329 325 Size: 1498 Fragments arranged by size: 1539 1498 1368 1255 445 185 153 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2208 4748 6460 Size: 274 80 33 51 1443 231 2540 1712 Cuts at: 6460 96 Size: 138 Fragments arranged by size: 2540 1712 1443 274 231 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2764 2796 6462 6494 2764 Size: 32 3666 32 2772 Fragments arranged by size: 3666 2772 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6502 XbaI T'CTAG_A Cuts at: 2450 3838 2450 Size: 1388 5114 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3709 3709 Size: 6502 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6502 XmaI C'CCGG_G Cuts at: 1870 2049 3827 1870 Size: 179 1778 4545 Fragments arranged by size: 4545 1778 179 XmnI GAAnn'nnTTC Cuts at: 2624 4631 2624 Size: 2007 4495 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4310 276 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI