Restriction map for
pFN21AB7860
(Linear) (Six-base) MAPSORT of: KIBB7860.seq Check: 4847 from: 1 to: 1797
KIBB7860.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1181 1797
Size: 1181 616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 155 745 806 1310 1522 1551 1678
Size: 55 100 590 61 504 212 29 127
Cuts at: 1678 1797
Size: 119
Fragments arranged by size:
590 504 212 127 119 100 61 55
29
AleI CACnn'nnGTG
Cuts at: 0 1442 1797
Size: 1442 355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 1797
Size: 31 1766
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1099 1131 1797
Size: 1099 32 666
Fragments arranged by size:
1099 666 32
ApaLI G'TGCA_C
Cuts at: 0 1762 1797
Size: 1762 35
ApoI r'AATT_y
Cuts at: 0 441 592 619 889 1396 1797
Size: 441 151 27 270 507 401
Fragments arranged by size:
507 441 401 270 151 27
AvaI C'yCGr_G
Cuts at: 0 44 1797
Size: 44 1753
BanI G'GyrC_C
Cuts at: 0 261 1797
Size: 261 1536
BanII G_rGCy'C
Cuts at: 0 68 1797
Size: 68 1729
BbsI GAAGACnn'nnnn_
Cuts at: 0 143 456 1028 1099 1797
Size: 143 313 572 71 698
Fragments arranged by size:
698 572 313 143 71
BbvCI CC'TCA_GC
Cuts at: 0 389 1797
Size: 389 1408
BciVI GTATCCnnnnn_n'
Cuts at: 0 1233 1797
Size: 1233 564
BclI T'GATC_A
Cuts at: 0 923 987 1797
Size: 923 64 810
Fragments arranged by size:
923 810 64
BlpI GC'TnA_GC
Cuts at: 0 548 1797
Size: 548 1249
Bme1580I G_kGCm'C
Cuts at: 0 1766 1797
Size: 1766 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 619 1797
Size: 619 1178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 541 791 1631 1797
Size: 137 404 250 840 166
Fragments arranged by size:
840 404 250 166 137
Bpu10I CC'TnA_GC
Cuts at: 0 389 1797
Size: 389 1408
BsaI GGTCTCn'nnnn_
Cuts at: 0 1104 1797
Size: 1104 693
BsaAI yAC'GTr
Cuts at: 0 15 1797
Size: 15 1782
BsaWI w'CCGG_w
Cuts at: 0 1145 1430 1797
Size: 1145 285 367
Fragments arranged by size:
1145 367 285
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 13 43 1099 1129 1522 1552 1797
Size: 13 30 1056 30 393 30 245
Fragments arranged by size:
1056 393 245 30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 406 598 1120 1797
Size: 406 192 522 677
Fragments arranged by size:
677 522 406 192
BseYI C'CCAG_C
Cuts at: 0 3 1797
Size: 3 1794
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1575 1797
Size: 1575 222
BsiHKAI G_wGCw'C
Cuts at: 0 1002 1549 1595 1766 1797
Size: 1002 547 46 171 31
Fragments arranged by size:
1002 547 171 46 31
BsmI GAATG_Cn'
Cuts at: 0 134 1720 1797
Size: 134 1586 77
Fragments arranged by size:
1586 134 77
Bsp1286I G_dGCh'C
Cuts at: 0 68 1002 1549 1595 1766 1797
Size: 68 934 547 46 171 31
Fragments arranged by size:
934 547 171 68 46 31
BspEI T'CCGG_A
Cuts at: 0 1145 1430 1797
Size: 1145 285 367
Fragments arranged by size:
1145 367 285
BspHI T'CATG_A
Cuts at: 0 255 855 1269 1797
Size: 255 600 414 528
Fragments arranged by size:
600 528 414 255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1181 1797
Size: 1181 616
BsrDI GCAATG_nn'
Cuts at: 0 562 1797
Size: 562 1235
BsrGI T'GTAC_A
Cuts at: 0 203 1797
Size: 203 1594
BstAPI GCAn_nnn'nTGC
Cuts at: 0 639 1797
Size: 639 1158
BstYI r'GATC_y
Cuts at: 0 774 1797
Size: 774 1023
BtgI C'CryG_G
Cuts at: 0 226 1779 1797
Size: 226 1553 18
Fragments arranged by size:
1553 226 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1232 1797
Size: 1232 565
BtsI GCAGTG_nn'
Cuts at: 0 1212 1413 1797
Size: 1212 201 384
Fragments arranged by size:
1212 384 201
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 591 626 1029 1064 1797
Size: 591 35 403 35 733
Fragments arranged by size:
733 591 403 35 35
EaeI y'GGCC_r
Cuts at: 0 1389 1797
Size: 1389 408
EarI CTCTTCn'nnn_
Cuts at: 0 118 721 1797
Size: 118 603 1076
Fragments arranged by size:
1076 603 118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 137 155 541 745 791 806 1310
Size: 55 82 18 386 204 46 15 504
Cuts at: 1310 1522 1551 1631 1678 1797
Size: 212 29 80 47 119
Fragments arranged by size:
504 386 212 204 119 82 80 55
47 46 29 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1230 1797
Size: 1230 567
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 496 1797
Size: 496 1301
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 250 282 972 1004 1412 1444
Size: 13 32 205 32 690 32 408 32
Cuts at: 1444 1677 1709 1797
Size: 233 32 88
Fragments arranged by size:
690 408 233 205 88 32 32 32
32 32 13
HindIII A'AGCT_T
Cuts at: 0 787 1797
Size: 787 1010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1797
Size: 260 1537
MscI TGG'CCA
Cuts at: 0 1391 1797
Size: 1391 406
MslI CAynn'nnrTG
Cuts at: 0 13 1070 1187 1217 1442 1797
Size: 13 1057 117 30 225 355
Fragments arranged by size:
1057 355 225 117 30 13
MspA1I CmG'CkG
Cuts at: 0 463 710 1797
Size: 463 247 1087
Fragments arranged by size:
1087 463 247
NcoI C'CATG_G
Cuts at: 0 1779 1797
Size: 1779 18
NdeI CA'TA_TG
Cuts at: 0 920 1797
Size: 920 877
NspI r_CATG'y
Cuts at: 0 12 1797
Size: 12 1785
PflMI CCAn_nnn'nTGG
Cuts at: 0 1387 1797
Size: 1387 410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1099 1131 1797
Size: 1099 32 666
Fragments arranged by size:
1099 666 32
PvuII CAG'CTG
Cuts at: 0 710 1797
Size: 710 1087
SapI GCTCTTCn'nnn_
Cuts at: 0 721 1797
Size: 721 1076
SfcI C'TryA_G
Cuts at: 0 835 1797
Size: 835 962
SmaI CCC'GGG
Cuts at: 0 46 1797
Size: 46 1751
StuI AGG'CCT
Cuts at: 0 1312 1564 1583 1797
Size: 1312 252 19 214
Fragments arranged by size:
1312 252 214 19
StyI C'CwwG_G
Cuts at: 0 480 1779 1797
Size: 480 1299 18
Fragments arranged by size:
1299 480 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 943 985 1797
Size: 943 42 812
Fragments arranged by size:
943 812 42
TatI w'GTAC_w
Cuts at: 0 203 1797
Size: 203 1594
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 759 791 1797
Size: 759 32 1006
Fragments arranged by size:
1006 759 32
XbaI T'CTAG_A
Cuts at: 0 445 1797
Size: 445 1352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1704 1797
Size: 1704 93
XmaI C'CCGG_G
Cuts at: 0 44 1797
Size: 44 1753
XmnI GAAnn'nnTTC
Cuts at: 0 619 1797
Size: 619 1178
Enzymes that do cut:
AarI AcuI AleI AlfI AloI ApaLI ApoI AvaI
BanI BanII BbsI BbvCI BciVI BclI BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrGI BstAPI BstYI BtgI BtgZI BtsI CspCI
EaeI EarI Eco57MI EcoNI FalI Hin4I HindIII MmeI
MscI MslI MspA1I NcoI NdeI NspI PflMI PpiI
PvuII SapI SfcI SmaI StuI StyI TaqII TatI
TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BfrBI BglI BglII BmgBI BmtI
BplI BpuEI BsaBI BsaHI BsiEI BsiWI BsmBI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XhoI ZraI