Restriction map for
pFN21AB6411
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# NO CUT ENZYMES ( 129 enzymes) #
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Acc65I AccI AccII AccIII AcsI
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BalI BamHI
BbeI BbrPI BciVI BclI BfaI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 211 GACGTC GACGT^C
AclI 276 AACGTT AA^CGTT
AcyI 211 GACGTC G[GA]^CG[CT]C
AfaI 399 GTAC GT^AC
AflII 298 CTTAAG C^TTAAG
Alw44I 487 GTGCAC G^TGCAC
AlwNI 514 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 568 GGGCCC GGGCC^C
ApaLI 487 GTGCAC G^TGCAC
Asp700I 840 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 629 CCCGAG C^[CT]CG[GA]G
AviII 392 TGCGCA TGC^GCA
BbsI 601 GAAGAC GAAGAC(2/6)
BbvCI 447 CCTCAGC CCTCAGC(-5/-2)
BcgI 36 GCAGCTGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 333 CCGGG CC^[GC]GG
BfrI 298 CTTAAG C^TTAAG
BpmI 835 CTCCAG CTGGAG(16/14)
BpuAI 601 GAAGAC GAAGAC(2/6)
BsaHI 211 GACGTC G[GA]^CG[CT]C
BsaI 220 GAGACC GGTCTC(1/5)
BsiEI 540 CGACCG CG[GA][CT]^CG
BsmBI 751 GAGACG CGTCTC(1/5)
BsoBI 629 CCCGAG C^[CT]CG[GA]G
BspDI 466 ATCGAT AT^CGAT
BspHI 561 TCATGA T^CATGA
BspMI 363 GCAGGT ACCTGC(4/8)
BstAPI 549 GCAAGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 466 ATCGAT AT^CGAT
DrdI 136 GACTCGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 905 TGGCCG [CT]^GGCC[GA]
EarI 232 CTCTTC CTCTTC(1/4)
EcoT14I 165 CCATGG C^C[AT][AT]GG
FspI 392 TGCGCA TGC^GCA
HaeII 419 GGCGCT [GA]GCGC^[CT]
Hin1I 211 GACGTC G[GA]^CG[CT]C
Ksp632I 232 CTCTTC CTCTTC(1/4)
MseI 299 TTAA T^TAA
NaeI 637 GCCGGC GCC^GGC
NciI 333 CCGGG CC^[GC]GG
NcoI 165 CCATGG C^CATGG
NgoMIV 637 GCCGGC G^CCGGC
Psp1406I 276 AACGTT AA^CGTT
PvuII 37 CAGCTG CAG^CTG
RcaI 561 TCATGA T^CATGA
RsaI 399 GTAC GT^AC
SacI 52 GAGCTC GAGCT^C
SfcI 264 CTATAG C^T[GA][CT]AG
SmlI 298 CTTAAG C^T[CT][GA]AG
StyI 165 CCATGG C^C[AT][AT]GG
TfiI 528 GATTC G^A[AT]TC
Tru9I 299 TTAA T^TAA
Tsp509I 173 AATT ^AATT
XmnI 840 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 330 GATCC GGATC(4/5)
522 GGATC
AvaII 21 GGACC G^G[AT]CC
252 GGACC
BanI 555 GGTGCC G^G[CT][GA]CC
774 GGCACC
BsgI 426 CTGCAC GTGCAG(16/14)
124 GTGCAG
BsmFI 242 GGGAC GGGAC(10/14)
214 GTCCC
BsrFI 23 ACCGGC [GA]^CCGG[CT]
637 GCCGGC
BstNI 18 CCTGG CC^[AT]GG
519 CCTGG
Cfr10I 23 ACCGGC [GA]^CCGG[CT]
637 GCCGGC
DraII 77 AGGCCCT [GA]G^G[ACGT]CC[CT]
494 GGGGCCT
DraIII 147 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
762 CACTGTGTG
EcoO109I 77 AGGCCCT [GA]G^G[ACGT]CC[CT]
494 GGGGCCT
EcoRII 18 CCTGG ^CC[AT]GG
519 CCTGG
HgaI 42 GCGTC GACGC(5/10)
269 GCGTC
HphI 408 GGTGA GGTGA(8/7)
720 GGTGA
MslI 690 CATTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
742 CACTTCAATG
MspA1I 37 CAGCTG C[AC]G^C[GT]G
792 CCGCTG
MvaI 18 CCTGG CC^[AT]GG
519 CCTGG
PleI 136 GACTC GAGTC(4/5)
348 GAGTC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AspHI BanII BbvI
BglII BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboI MboII MflI MnlI MspI
MwoI NdeII NlaIII NlaIV PflMI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII