(Circular) (Six-base) MAPSORT of: pFN21AB6411.seq Check: 9389 from: 1 to: 5626 pFN21AB6411 5626 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2220 3436 278 Size: 53 83 186 936 684 1216 2468 Fragments arranged by size: 2468 1216 936 684 186 83 53 AccI GT'mk_AC Cuts at: 1937 2969 1937 Size: 1032 4594 Acc65I G'GTAC_C Cuts at: 2947 2947 Size: 5626 AclI AA'CG_TT Cuts at: 1563 2282 3753 4126 1563 Size: 719 1471 373 3063 Fragments arranged by size: 3063 1471 719 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3689 5073 5341 1632 Size: 2057 1384 268 1917 Fragments arranged by size: 2057 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2303 820 Size: 197 1286 4143 Fragments arranged by size: 4143 1286 197 AflIII A'CryG_T Cuts at: 1203 4525 5502 1203 Size: 3322 977 1327 Fragments arranged by size: 3322 1327 977 AgeI A'CCGG_T Cuts at: 4457 4457 Size: 5626 AhdI GACnn_n'nnGTC Cuts at: 4355 4355 Size: 5626 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5626 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5626 AlwNI CAG_nnn'CTG Cuts at: 2524 3369 4941 2524 Size: 845 1572 3209 Fragments arranged by size: 3209 1572 845 ApaI G_GGCC'C Cuts at: 2577 2577 Size: 5626 ApaLI G'TGCA_C Cuts at: 2492 3683 4839 2492 Size: 1191 1156 3279 Fragments arranged by size: 3279 1191 1156 ApoI r'AATT_y Cuts at: 1493 2935 3161 3313 5422 1493 Size: 1442 226 152 2109 1697 Fragments arranged by size: 2109 1697 1442 226 152 AseI AT'TA_AT Cuts at: 160 1032 4180 160 Size: 872 3148 1606 Fragments arranged by size: 3148 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 AvaI C'yCGr_G Cuts at: 1870 1943 2634 2951 1870 Size: 73 691 317 4545 Fragments arranged by size: 4545 691 317 73 BamHI G'GATC_C Cuts at: 2956 3323 2956 Size: 367 5259 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2560 2779 2947 4402 618 Size: 325 217 627 773 219 168 1455 1842 Fragments arranged by size: 1842 1455 773 627 325 219 217 168 BanII G_rGCy'C Cuts at: 1391 2061 2577 2633 2945 1391 Size: 670 516 56 312 4072 Fragments arranged by size: 4072 670 516 312 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2613 928 Size: 971 714 3941 Fragments arranged by size: 3941 971 714 BbvCI CC'TCA_GC Cuts at: 2453 2453 Size: 5626 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2030 2064 3817 3851 1903 Size: 34 93 34 1753 34 3678 Fragments arranged by size: 3678 1753 93 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3518 4728 3518 Size: 1210 4416 BclI T'GATC_A Cuts at: 1548 1548 Size: 5626 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4237 136 Size: 107 122 71 631 605 2565 1525 Fragments arranged by size: 2565 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2106 2439 2481 5621 2106 Size: 333 42 3140 2111 Fragments arranged by size: 3140 2111 333 42 BlpI GC'TnA_GC Cuts at: 3043 3043 Size: 5626 Bme1580I G_kGCm'C Cuts at: 1790 2496 2577 2716 3687 4843 1790 Size: 706 81 139 971 1156 2573 Fragments arranged by size: 2573 1156 971 706 139 81 BmgBI CAC'GTC Cuts at: 1318 2665 1318 Size: 1347 4279 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2668 3357 4315 Size: 416 53 83 399 24 1251 689 958 Cuts at: 4315 442 Size: 1753 Fragments arranged by size: 1753 1251 958 689 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5626 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2825 4286 1239 Size: 1586 1461 2579 Fragments arranged by size: 2579 1586 1461 Bpu10I CC'TnA_GC Cuts at: 2094 2453 2094 Size: 359 5267 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3751 4616 4914 3751 Size: 865 298 4463 Fragments arranged by size: 4463 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2219 4289 882 Size: 617 720 2070 2219 Fragments arranged by size: 2219 2070 720 617 BsaAI yAC'GTr Cuts at: 493 1204 3417 493 Size: 711 2213 2702 Fragments arranged by size: 2702 2213 711 BsaBI GATnn'nnATC Cuts at: 5620 5620 Size: 5626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2217 3433 3815 4450 Size: 53 83 186 936 684 1216 382 635 Cuts at: 4450 275 Size: 1451 Fragments arranged by size: 1451 1216 936 684 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4059 4457 4731 4878 5289 4059 Size: 398 274 147 411 4396 Fragments arranged by size: 4396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5401 Fragments arranged by size: 5401 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2579 2652 1186 Size: 946 447 73 4160 Fragments arranged by size: 4160 946 447 73 BseYI C'CCAG_C Cuts at: 2619 4829 5325 2619 Size: 2210 496 2920 Fragments arranged by size: 2920 2210 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2416 1163 Size: 573 414 266 4373 Fragments arranged by size: 4373 573 414 266 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2548 3076 3837 4865 1540 Size: 275 186 547 528 761 1028 2301 Fragments arranged by size: 2301 1028 761 547 528 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 2496 2766 3687 3772 4843 2061 Size: 435 270 921 85 1071 2844 Fragments arranged by size: 2844 1071 921 435 270 85 BsmI GAATG_Cn' Cuts at: 3137 3230 3137 Size: 93 5533 BsmBI CGTCTCn'nnnn_ Cuts at: 2750 2750 Size: 5626 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2496 2577 2633 2716 2766 2945 Size: 399 271 435 81 56 83 50 179 Cuts at: 2945 3687 3772 4843 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 271 179 85 83 81 56 50 BspEI T'CCGG_A Cuts at: 5289 5289 Size: 5626 BspHI T'CATG_A Cuts at: 2566 3515 5245 2566 Size: 949 1730 2947 Fragments arranged by size: 2947 1730 949 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2359 2981 844 Size: 1515 622 3489 Fragments arranged by size: 3489 1515 622 BsrBI CCG'CTC Cuts at: 1394 1823 3513 1394 Size: 429 1690 3507 Fragments arranged by size: 3507 1690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4121 4295 65 Size: 1183 2873 174 1396 Fragments arranged by size: 2873 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2028 2642 4270 4457 1700 Size: 110 218 614 1628 187 2869 Fragments arranged by size: 2869 1628 614 218 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5626 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5626 BssSI C'ACGA_G Cuts at: 3686 4698 3686 Size: 1012 4614 BstAPI GCAn_nnn'nTGC Cuts at: 794 2560 794 Size: 1766 3860 BstBI TT'CG_AA Cuts at: 4430 4430 Size: 5626 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5626 BstYI r'GATC_y Cuts at: 1972 2106 2439 2481 2956 3323 3708 3725 5177 Size: 134 333 42 475 367 385 17 1452 Cuts at: 5177 5263 5275 5621 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 475 385 367 346 333 134 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2170 2260 2907 513 Size: 552 939 166 90 647 3232 Fragments arranged by size: 3232 939 647 552 166 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4887 Fragments arranged by size: 4887 683 56 BtsI GCAGTG_nn' Cuts at: 2665 3142 3936 3956 2665 Size: 477 794 20 4335 Fragments arranged by size: 4335 794 477 20 ClaI AT'CG_AT Cuts at: 1554 2472 1554 Size: 918 4708 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5591 DraI TTT'AAA Cuts at: 2930 2930 Size: 5626 DraIII CAC_nnn'GTG Cuts at: 2157 2772 2157 Size: 615 5011 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2147 4633 809 Size: 507 831 2486 1802 Fragments arranged by size: 2486 1802 831 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2910 3073 3962 Size: 54 1425 50 275 18 1080 163 889 Cuts at: 3962 5591 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1080 889 275 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3073 1537 Size: 275 1261 4090 Fragments arranged by size: 4090 1261 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2243 3556 1353 Size: 890 1313 3423 Fragments arranged by size: 3423 1313 890 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4213 4587 4733 387 Size: 1064 138 435 2189 374 146 1280 Fragments arranged by size: 2189 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2059 2059 Size: 5626 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2825 3689 4286 5073 5341 1239 Size: 393 1193 864 597 787 268 1524 Fragments arranged by size: 1524 1193 864 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2083 2500 2083 Size: 417 5209 EcoRI G'AATT_C Cuts at: 2935 3313 2935 Size: 378 5248 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2764 2764 Size: 5626 FspI TGC'GCA Cuts at: 2399 4132 2399 Size: 1733 3893 HaeII r_GCGC'y Cuts at: 1678 2428 4773 1678 Size: 750 2345 2531 Fragments arranged by size: 2531 2345 750 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2512 2544 4267 4299 4341 Size: 32 135 32 940 32 1723 32 42 Cuts at: 4341 4373 1373 Size: 32 2626 Fragments arranged by size: 2626 1723 940 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2970 3216 669 Size: 988 281 1032 246 3079 Fragments arranged by size: 3079 1032 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5626 HpaI GTT'AAC Cuts at: 3216 3216 Size: 5626 KpnI G_GTAC'C Cuts at: 2951 2951 Size: 5626 MfeI C'AATT_G Cuts at: 3225 3225 Size: 5626 MluI A'CGCG_T Cuts at: 5502 5502 Size: 5626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4740 4924 1305 Size: 3435 184 2007 Fragments arranged by size: 3435 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3804 Fragments arranged by size: 3804 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2699 2751 3584 3943 4102 518 Size: 648 1533 52 833 359 159 2042 Fragments arranged by size: 2042 1533 833 648 359 159 52 MspA1I CmG'CkG Cuts at: 1606 2044 2799 3042 3719 4867 5112 1606 Size: 438 755 243 677 1148 245 2120 Fragments arranged by size: 2120 1148 755 677 438 245 243 NaeI GCC'GGC Cuts at: 2644 2644 Size: 5626 NcoI C'CATG_G Cuts at: 513 1065 2004 2170 513 Size: 552 939 166 3969 Fragments arranged by size: 3969 939 552 166 NdeI CA'TA_TG Cuts at: 387 387 Size: 5626 NgoMIV G'CCGG_C Cuts at: 2642 2642 Size: 5626 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5626 NotI GC'GGCC_GC Cuts at: 3073 3073 Size: 5626 NspI r_CATG'y Cuts at: 1132 1229 2984 4529 1132 Size: 97 1755 1545 2229 Fragments arranged by size: 2229 1755 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5626 PciI A'CATG_T Cuts at: 4525 4525 Size: 5626 PflMI CCAn_nnn'nTGG Cuts at: 1108 2524 2619 2722 1108 Size: 1416 95 103 4012 Fragments arranged by size: 4012 1416 103 95 PmeI GTTT'AAAC Cuts at: 2930 2930 Size: 5626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3695 3727 5208 5240 3695 Size: 32 1481 32 4081 Fragments arranged by size: 4081 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5626 PsiI TTA'TAA Cuts at: 3196 3196 Size: 5626 PspOMI G'GGCC_C Cuts at: 2573 2573 Size: 5626 PstI C_TGCA'G Cuts at: 830 2978 830 Size: 2148 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5626 PvuII CAG'CTG Cuts at: 2044 2044 Size: 5626 SacI G_AGCT'C Cuts at: 2061 2061 Size: 5626 SalI G'TCGA_C Cuts at: 1936 2968 1936 Size: 1032 4594 SbfI CC_TGCA'GG Cuts at: 2978 2978 Size: 5626 ScaI AGT'ACT Cuts at: 3874 5586 3874 Size: 1712 3914 SfcI C'TryA_G Cuts at: 826 1045 2269 2974 4109 4790 4981 826 Size: 219 1224 705 1135 681 191 1471 Fragments arranged by size: 1471 1224 1135 705 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5626 SmaI CCC'GGG Cuts at: 1872 2953 1872 Size: 1081 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2303 3730 4631 4893 820 Size: 197 926 360 1427 901 262 1553 Fragments arranged by size: 1553 1427 926 901 360 262 197 SnaBI TAC'GTA Cuts at: 493 3417 493 Size: 2924 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5626 SphI G_CATG'C Cuts at: 1132 2984 1132 Size: 1852 3774 SspI AAT'ATT Cuts at: 5 52 3550 5 Size: 47 3498 2081 Fragments arranged by size: 3498 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5626 StyI C'CwwG_G Cuts at: 513 1065 2004 2170 3065 513 Size: 552 939 166 895 3074 Fragments arranged by size: 3074 939 895 552 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2562 2797 3653 3670 3823 4008 4453 Size: 1255 982 235 856 17 153 185 445 Cuts at: 4453 325 Size: 1498 Fragments arranged by size: 1498 1255 982 856 445 235 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3872 5584 96 Size: 274 80 33 51 1443 1895 1712 138 Fragments arranged by size: 1895 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2874 2906 5586 5618 2874 Size: 32 2680 32 2882 Fragments arranged by size: 2882 2680 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5626 XbaI T'CTAG_A Cuts at: 2962 2962 Size: 5626 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2107 2521 2824 2905 2107 Size: 414 303 81 4828 Fragments arranged by size: 4828 414 303 81 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5626 XmaI C'CCGG_G Cuts at: 1870 2951 1870 Size: 1081 4545 XmnI GAAnn'nnTTC Cuts at: 2849 3755 2849 Size: 906 4720 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2218 3434 276 Size: 53 83 186 936 684 1216 2468 Fragments arranged by size: 2468 1216 936 684 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI