Restriction map for
pFN21AB7775
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspHI AvaI AvaII AviII AvrII
BanII BbrPI BbvCI BcgI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspEI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FseI FspI HincII HindII HindIII
HpaI KspI MfeI MluI MroI
MslI MspA1I MunI MvnI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SexAI
SfaNI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I StuI
SwaI Tsp45I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 557 GGTACC G^GTACC
Asp718I 557 GGTACC G^GTACC
AspI 994 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 837 TGGCCA TGG^CCA
BamHI 1203 GGATCC G^GATCC
BbeI 26 GGCGCC GGCGC^C
BbsI 925 GTCTTC GAAGAC(2/6)
BciVI 1075 GGATAC GTATCC(6/5)
BclI 350 TGATCA T^GATCA
BcnI 170 CCCGG CC^[GC]GG
BpmI 389 CTGGAG CTGGAG(16/14)
BpuAI 925 GTCTTC GAAGAC(2/6)
BsaBI 856 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 658 TGTACA T^GTACA
BspDI 1273 ATCGAT AT^CGAT
BspHI 491 TCATGA T^CATGA
BspMI 1026 ACCTGC ACCTGC(4/8)
BsrGI 658 TGTACA T^GTACA
BstNI 945 CCAGG CC^[AT]GG
CfoI 27 GCGC GCG^C
Cfr13I 1112 GGGCC G^G[ACGT]CC
ClaI 1273 ATCGAT AT^CGAT
DsaI 1137 CCATGG C^C[GA][CT]GG
EaeI 837 TGGCCA [CT]^GGCC[GA]
EarI 1220 CTCTTC CTCTTC(1/4)
EcoRI 151 GAATTC G^AATTC
EcoRII 945 CCAGG ^CC[AT]GG
FbaI 350 TGATCA T^GATCA
HaeII 26 GGCGCC [GA]GCGC^[CT]
HapII 171 CCGG C^CGG
HgaI 36 GACGC GACGC(5/10)
HhaI 27 GCGC GCG^C
HinP1I 27 GCGC G^CGC
HpaII 171 CCGG C^CGG
HphI 379 GGTGA GGTGA(8/7)
KasI 26 GGCGCC G^GCGCC
KpnI 557 GGTACC GGTAC^C
Ksp632I 1220 CTCTTC CTCTTC(1/4)
MaeII 607 ACGT A^CGT
MaeIII 1258 GTAAC ^GT[ACGT]AC
MamI 856 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 837 TGGCCA TGG^CCA
MscI 837 TGGCCA TGG^CCA
MspI 171 CCGG C^CGG
MvaI 945 CCAGG CC^[AT]GG
NarI 26 GGCGCC GG^CGCC
NciI 170 CCCGG CC^[GC]GG
NcoI 1137 CCATGG C^CATGG
NdeI 452 CATATG CA^TATG
PflFI 994 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 918 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 491 TCATGA T^CATGA
Sau96I 1112 GGGCC G^G[ACGT]CC
ScaI 842 AGTACT AGT^ACT
SfoI 26 GGCGCC GGC^GCC
SspBI 658 TGTACA T^GTACA
SspI 110 AATATT AAT^ATT
TaqI 1274 TCGA T^CGA
TspRI 1329 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 994 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1274 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 26 GGCGCC G[GA]^CG[CT]C
36 GACGCC
BanI 26 GGCGCC G^G[CT][GA]CC
557 GGTACC
BfaI 31 CTAG C^TAG
745 CTAG
BsaHI 26 GGCGCC G[GA]^CG[CT]C
36 GACGCC
BsiYI 164 CCATTCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
328 CCTATGATTGG
BslI 164 CCATTCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
328 CCTATGATTGG
BsmAI 1250 GAGAC GTCTC(1/5)
953 GTCTC
BsrI 5 ACTGG ACTGG(1/-1)
840 CCAGT
BssKI 170 CCCGG ^CC[ACGT]GG
945 CCAGG
BstF5I 768 GGATG GGATG(2/0)
1173 GGATG
EcoT14I 782 CCAAGG C^C[AT][AT]GG
1137 CCATGG
FokI 768 GGATG GGATG(9/13)
1173 GGATG
HaeIII 838 GGCC GG^CC
1113 GGCC
Hin1I 26 GGCGCC G[GA]^CG[CT]C
36 GACGCC
MaeI 31 CTAG C^TAG
745 CTAG
MwoI 814 GCTCCTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1312 GCTCTTGTTGC
NspI 733 GCATGC [GA]CATG^[CT]
872 GCATGC
PleI 527 GACTC GAGTC(4/5)
789 GAGTC
PstI 1028 CTGCAG CTGCA^G
1340 CTGCAG
ScrFI 170 CCCGG CC^[ACGT]GG
945 CCAGG
SphI 733 GCATGC GCATG^C
872 GCATGC
StyI 782 CCAAGG C^C[AT][AT]GG
1137 CCATGG
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbvI BsaJI BstYI Cac8I
DdeI DpnII Fnu4HI HinfI ItaI
MboI MboII MflI MnlI MseI
NdeII NlaIII NlaIV RsaI Sau3AI
SfcI TfiI Tru9I TseI Tsp509I
XhoII XmnI