(Circular) (Six-base) MAPSORT of: pFN21AB7775.seq Check: 9958 from: 1 to: 6064 pFN21AB7775 6064 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3407 1937 Size: 1470 4594 Acc65I G'GTAC_C Cuts at: 2562 3385 2562 Size: 823 5241 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2220 3212 4127 5511 5779 1632 Size: 588 992 915 1384 268 1917 Fragments arranged by size: 1917 1384 992 915 588 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 4963 5940 1203 Size: 3760 977 1327 Fragments arranged by size: 3760 1327 977 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 4793 4793 Size: 6064 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AlwNI CAG_nnn'CTG Cuts at: 3807 5379 3807 Size: 1572 4492 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 2156 2466 2721 3373 3599 3751 5860 1493 Size: 663 310 255 652 226 152 2109 1697 Fragments arranged by size: 2109 1697 663 652 310 255 226 152 AseI AT'TA_AT Cuts at: 160 1032 4618 160 Size: 872 3586 1606 Fragments arranged by size: 3586 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 3389 1870 Size: 73 1446 4545 Fragments arranged by size: 4545 1446 73 BamHI G'GATC_C Cuts at: 3208 3394 3761 3208 Size: 186 367 5511 Fragments arranged by size: 5511 367 186 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2031 2562 3385 4840 618 Size: 325 217 627 244 531 823 1455 1842 Fragments arranged by size: 1842 1455 823 627 531 325 244 217 BanII G_rGCy'C Cuts at: 1391 3383 1391 Size: 1992 4072 BbeI G_GCGC'C Cuts at: 2035 2035 Size: 6064 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2923 928 Size: 971 1024 4069 Fragments arranged by size: 4069 1024 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4255 4289 1903 Size: 34 2318 34 3678 Fragments arranged by size: 3678 2318 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3074 3956 5166 3074 Size: 882 1210 3972 Fragments arranged by size: 3972 1210 882 BclI T'GATC_A Cuts at: 1548 2355 1548 Size: 807 5257 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4675 136 Size: 107 122 71 631 605 3003 1525 Fragments arranged by size: 3003 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6059 6059 Size: 6064 BlpI GC'TnA_GC Cuts at: 3481 3481 Size: 6064 Bme1580I G_kGCm'C Cuts at: 1790 4125 5281 1790 Size: 2335 1156 2573 Fragments arranged by size: 2573 2335 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3795 4753 442 Size: 416 53 83 399 24 2378 958 1753 Fragments arranged by size: 2378 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2415 4724 1239 Size: 1176 2309 2579 Fragments arranged by size: 2579 2309 1176 Bpu10I CC'TnA_GC Cuts at: 2768 2768 Size: 6064 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4189 5054 5352 4189 Size: 865 298 4901 Fragments arranged by size: 4901 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4727 882 Size: 617 3228 2219 Fragments arranged by size: 3228 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3855 493 Size: 711 2651 2702 Fragments arranged by size: 2702 2651 711 BsaBI GATnn'nnATC Cuts at: 2865 6058 2865 Size: 3193 2871 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2032 2042 3871 4253 Size: 53 83 186 936 499 10 1829 382 Cuts at: 4253 4888 275 Size: 635 1451 Fragments arranged by size: 1829 1451 936 635 499 382 186 83 53 10 BsaWI w'CCGG_w Cuts at: 4497 4895 5169 5316 5727 4497 Size: 398 274 147 411 4834 Fragments arranged by size: 4834 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3246 3276 1178 Size: 30 165 30 1843 30 3966 Fragments arranged by size: 3966 1843 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6064 BseYI C'CCAG_C Cuts at: 2552 5267 5763 2552 Size: 2715 496 2853 Fragments arranged by size: 2853 2715 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5491 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4125 4210 5281 4125 Size: 85 1071 4908 Fragments arranged by size: 4908 1071 85 BsmI GAATG_Cn' Cuts at: 3575 3668 3575 Size: 93 5971 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3383 4125 4210 5281 1391 Size: 399 1593 742 85 1071 2174 Fragments arranged by size: 2174 1593 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 2496 3953 5683 2496 Size: 1457 1730 2877 Fragments arranged by size: 2877 1730 1457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3040 3419 844 Size: 2196 379 3489 Fragments arranged by size: 3489 2196 379 BsrBI CCG'CTC Cuts at: 1394 1823 3951 1394 Size: 429 2128 3507 Fragments arranged by size: 3507 2128 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4559 4733 65 Size: 1183 3311 174 1396 Fragments arranged by size: 3311 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4708 4895 1700 Size: 110 2898 187 2869 Fragments arranged by size: 2898 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2663 96 Size: 2567 3497 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6064 BssSI C'ACGA_G Cuts at: 4124 5136 4124 Size: 1012 5052 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6064 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6064 BstYI r'GATC_y Cuts at: 1972 2695 3151 3208 3394 3761 4146 4163 5615 Size: 723 456 57 186 367 385 17 1452 Cuts at: 5615 5701 5713 6059 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 723 456 385 367 346 186 86 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3142 513 Size: 552 939 1138 3435 Fragments arranged by size: 3435 1138 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2366 487 Size: 683 56 1140 4185 Fragments arranged by size: 4185 1140 683 56 BtsI GCAGTG_nn' Cuts at: 3580 4374 4394 3580 Size: 794 20 5250 Fragments arranged by size: 5250 794 20 ClaI AT'CG_AT Cuts at: 1554 3279 1554 Size: 1725 4339 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2842 3511 4400 Size: 54 1425 50 275 18 1012 669 889 Cuts at: 4400 6029 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1012 889 669 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3231 3994 1353 Size: 1878 763 3423 Fragments arranged by size: 3423 1878 763 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4651 5025 5171 387 Size: 1064 138 3062 374 146 1280 Fragments arranged by size: 3062 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2220 2415 3212 4127 4724 5511 5779 Size: 393 588 195 797 915 597 787 268 Cuts at: 5779 1239 Size: 1524 Fragments arranged by size: 1524 915 797 787 597 588 393 268 195 EcoRI G'AATT_C Cuts at: 2156 3373 3751 2156 Size: 1217 378 4469 Fragments arranged by size: 4469 1217 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2039 2528 2701 3010 2039 Size: 489 173 309 5093 Fragments arranged by size: 5093 489 309 173 FspI TGC'GCA Cuts at: 4570 4570 Size: 6064 HaeII r_GCGC'y Cuts at: 1678 2035 5211 1678 Size: 357 3176 2531 Fragments arranged by size: 3176 2531 357 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2768 2800 4705 4737 4779 Size: 32 135 32 1196 32 1905 32 42 Cuts at: 4779 4811 1373 Size: 32 2626 Fragments arranged by size: 2626 1905 1196 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3408 3654 669 Size: 988 281 1470 246 3079 Fragments arranged by size: 3079 1470 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 3654 3654 Size: 6064 KasI G'GCGC_C Cuts at: 2031 2031 Size: 6064 KpnI G_GTAC'C Cuts at: 2566 3389 2566 Size: 823 5241 MfeI C'AATT_G Cuts at: 3663 3663 Size: 6064 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5178 5362 1305 Size: 3873 184 2007 Fragments arranged by size: 3873 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2844 10 Size: 54 1425 343 1012 3230 Fragments arranged by size: 3230 1425 1012 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4022 4381 4540 518 Size: 648 2856 359 159 2042 Fragments arranged by size: 2856 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3480 4157 5305 5550 1606 Size: 1874 677 1148 245 2120 Fragments arranged by size: 2120 1874 1148 677 245 NarI GG'CG_CC Cuts at: 2032 2032 Size: 6064 NcoI C'CATG_G Cuts at: 513 1065 2004 3142 513 Size: 552 939 1138 3435 Fragments arranged by size: 3435 1138 939 552 NdeI CA'TA_TG Cuts at: 387 2458 387 Size: 2071 3993 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2742 2881 3422 4967 1132 Size: 97 1513 139 541 1545 2229 Fragments arranged by size: 2229 1545 1513 541 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 4963 4963 Size: 6064 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5162 PfoI T'CCnGG_A Cuts at: 2949 2949 Size: 6064 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2927 1613 Size: 1314 4750 PsiI TTA'TAA Cuts at: 3634 3634 Size: 6064 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3065 3097 3065 Size: 32 6032 PstI C_TGCA'G Cuts at: 830 3037 3349 3416 830 Size: 2207 312 67 3478 Fragments arranged by size: 3478 2207 312 67 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 2849 4312 6024 2849 Size: 1463 1712 2889 Fragments arranged by size: 2889 1712 1463 SfcI C'TryA_G Cuts at: 826 1045 2216 3033 3345 3412 4547 5228 5419 Size: 219 1171 817 312 67 1135 681 191 Cuts at: 5419 826 Size: 1471 Fragments arranged by size: 1471 1171 1135 817 681 312 219 191 67 SfoI GGC'GCC Cuts at: 2033 2033 Size: 6064 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 3391 1872 Size: 1519 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4168 5069 5331 820 Size: 197 926 2225 901 262 1553 Fragments arranged by size: 2225 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 2742 2881 3422 1132 Size: 1610 139 541 3774 Fragments arranged by size: 3774 1610 541 139 SspI AAT'ATT Cuts at: 5 52 2117 3988 5 Size: 47 2065 1871 2081 Fragments arranged by size: 2081 2065 1871 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6064 StyI C'CwwG_G Cuts at: 513 1065 2004 2787 3142 3503 513 Size: 552 939 783 355 361 3074 Fragments arranged by size: 3074 939 783 552 361 355 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4091 4108 4261 4446 4891 325 Size: 1255 2511 17 153 185 445 1498 Fragments arranged by size: 2511 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2663 2847 4310 Size: 274 80 33 51 1443 686 184 1463 Cuts at: 4310 6022 96 Size: 1712 138 Fragments arranged by size: 1712 1463 1443 686 274 184 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6024 6056 6024 Size: 32 6032 Tth111I GACn'n_nGTC Cuts at: 1316 3002 1316 Size: 1686 4378 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6064 XmaI C'CCGG_G Cuts at: 1870 3389 1870 Size: 1519 4545 XmnI GAAnn'nnTTC Cuts at: 2022 2467 2681 3290 4193 2022 Size: 445 214 609 903 3893 Fragments arranged by size: 3893 903 609 445 214 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI