Restriction map for
pFN21AB6458
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AviII AvrII BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 223 GTCTAC GT^[AC][GT]AC
AcsI 323 AAATTC [GA]^AATT[CT]
AfaI 269 GTAC GT^AC
AhdI 187 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 717 GGGCCC GGGCC^C
ApoI 323 AAATTC [GA]^AATT[CT]
AspEI 187 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 203 CTCGGG C^[CT]CG[GA]G
BalI 1018 TGGCCA TGG^CCA
BamHI 645 GGATCC G^GATCC
BanII 717 GGGCCC G[GA]GC[CT]^C
BbvCI 316 CCTCAGC CCTCAGC(-5/-2)
BcnI 73 CCCGG CC^[GC]GG
BfaI 26 CTAG C^TAG
BglII 258 AGATCT A^GATCT
BmyI 717 GGGCCC G[AGT]GC[ACT]^C
BpmI 736 CTGGAG CTGGAG(16/14)
BsaBI 640 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 342 GGTCTC GGTCTC(1/5)
BsoBI 203 CTCGGG C^[CT]CG[GA]G
Bsp1286I 717 GGGCCC G[AGT]GC[ACT]^C
BspDI 331 ATCGAT AT^CGAT
BspMI 58 GCAGGT ACCTGC(4/8)
BsrDI 395 CATTGC GCAATG(2/0)
BstXI 828 CCAGCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 331 ATCGAT AT^CGAT
DraIII 809 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1018 TGGCCA [CT]^GGCC[GA]
Eam1105I 187 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 843 CCATGG C^C[AT][AT]GG
FauI 33 CCCGC CCCGC(4/6)
HphI 600 GGTGA GGTGA(8/7)
MaeI 26 CTAG C^TAG
MamI 640 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1018 TGGCCA TGG^CCA
MscI 1018 TGGCCA TGG^CCA
MslI 82 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 73 CCCGG CC^[GC]GG
NcoI 843 CCATGG C^CATGG
NspI 758 GCATGC [GA]CATG^[CT]
RsaI 269 GTAC GT^AC
SmlI 448 CTCAAG C^T[CT][GA]AG
SphI 758 GCATGC GCATG^C
StyI 843 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 12 CGCG CG^CG
379 CGCG
AluI 485 AGCT AG^CT
975 AGCT
AlwI 646 GATCC GGATC(4/5)
645 GGATC
AvaII 207 GGTCC G^G[AT]CC
690 GGTCC
BbvI 7 GCAGC GCAGC(8/12)
214 GCTGC
BseRI 472 GAGGAG GAGGAG(10/8)
547 GAGGAG
BsgI 215 CTGCAC GTGCAG(16/14)
944 CTGCAC
BsmAI 101 GAGAC GTCTC(1/5)
343 GTCTC
BsmFI 847 GGGAC GGGAC(10/14)
691 GTCCC
BstUI 12 CGCG CG^CG
379 CGCG
BstYI 258 AGATCT [GA]^GATC[CT]
645 GGATCC
DsaI 338 CCACGG C^C[GA][CT]GG
843 CCATGG
EarI 240 GAAGAG CTCTTC(1/4)
582 GAAGAG
HapII 20 CCGG C^CGG
74 CCGG
HpaII 20 CCGG C^CGG
74 CCGG
Ksp632I 240 GAAGAG CTCTTC(1/4)
582 GAAGAG
MflI 258 AGATCT [GA]^GATC[CT]
645 GGATCC
MspA1I 34 CCGCTG C[AC]G^C[GT]G
777 CAGCGG
MspI 20 CCGG C^CGG
74 CCGG
MvnI 12 CGCG CG^CG
379 CGCG
PleI 785 GACTC GAGTC(4/5)
909 GACTC
PstI 683 CTGCAG CTGCA^G
956 CTGCAG
SfcI 683 CTGCAG C^T[GA][CT]AG
956 CTGCAG
TseI 7 GCAGC G^C[AT]GC
214 GCTGC
Tsp509I 111 AATT ^AATT
324 AATT
XhoII 258 AGATCT [GA]^GATC[CT]
645 GGATCC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI ItaI MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tsp45I TspRI
TthHB8I