(Circular) (Six-base) MAPSORT of: pFN21AB6458.seq Check: 4439 from: 1 to: 5758 pFN21AB6458 5758 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2229 3101 1937 Size: 292 872 4594 Fragments arranged by size: 4594 872 292 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2264 2764 2997 3821 5205 5473 1632 Size: 632 500 233 824 1384 268 1917 Fragments arranged by size: 1917 1384 824 632 500 268 233 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 2197 4487 2197 Size: 2290 3468 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2780 2790 1184 Size: 1596 10 4152 Fragments arranged by size: 4152 1596 10 AlwNI CAG_nnn'CTG Cuts at: 3501 5073 3501 Size: 1572 4186 ApaI G_GGCC'C Cuts at: 2726 2726 Size: 5758 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 2328 3067 3293 3445 5554 1493 Size: 835 739 226 152 2109 1697 Fragments arranged by size: 2109 1697 835 739 226 152 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2208 3083 1870 Size: 73 265 875 4545 Fragments arranged by size: 4545 875 265 73 BamHI G'GATC_C Cuts at: 2650 3088 3455 2650 Size: 438 367 4953 Fragments arranged by size: 4953 438 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3079 4534 618 Size: 325 217 627 1292 1455 1842 Fragments arranged by size: 1842 1455 1292 627 325 217 BanII G_rGCy'C Cuts at: 1391 2726 3077 1391 Size: 1335 351 4072 Fragments arranged by size: 4072 1335 351 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4787 BbvCI CC'TCA_GC Cuts at: 2322 2322 Size: 5758 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4369 136 Size: 107 122 71 631 605 337 2360 1525 Fragments arranged by size: 2360 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2263 5753 2263 Size: 3490 2268 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2726 3819 4975 1790 Size: 936 1093 1156 2573 Fragments arranged by size: 2573 1156 1093 936 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2474 2693 3489 Size: 416 53 83 399 24 1057 219 796 Cuts at: 3489 4447 442 Size: 958 1753 Fragments arranged by size: 1753 1057 958 796 416 399 219 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2762 4418 1239 Size: 1523 1656 2579 Fragments arranged by size: 2579 1656 1523 Bpu10I CC'TnA_GC Cuts at: 2322 2322 Size: 5758 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2438 3883 4748 5046 2438 Size: 1445 865 298 3150 Fragments arranged by size: 3150 1445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2353 4421 882 Size: 617 854 2068 2219 Fragments arranged by size: 2219 2068 854 617 BsaAI yAC'GTr Cuts at: 493 1204 3549 493 Size: 711 2345 2702 Fragments arranged by size: 2702 2345 711 BsaBI GATnn'nnATC Cuts at: 2649 5752 2649 Size: 3103 2655 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3565 3947 4582 275 Size: 53 83 186 936 2032 382 635 1451 Fragments arranged by size: 2032 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4191 4589 4863 5010 5421 4191 Size: 398 274 147 411 4528 Fragments arranged by size: 4528 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5533 Fragments arranged by size: 5533 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2492 2567 1186 Size: 1306 75 4377 Fragments arranged by size: 4377 1306 75 BseYI C'CCAG_C Cuts at: 2832 4961 5457 2832 Size: 2129 496 3133 Fragments arranged by size: 3133 2129 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2205 2934 1163 Size: 573 469 729 3987 Fragments arranged by size: 3987 729 573 469 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3208 3969 4997 1540 Size: 275 186 1207 761 1028 2301 Fragments arranged by size: 2301 1207 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3819 3904 4975 3819 Size: 85 1071 4602 Fragments arranged by size: 4602 1071 85 BsmI GAATG_Cn' Cuts at: 3269 3362 3269 Size: 93 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2726 3077 3819 3904 4975 1391 Size: 399 936 351 742 85 1071 2174 Fragments arranged by size: 2174 1071 936 742 399 351 85 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 3113 844 Size: 1210 1059 3489 Fragments arranged by size: 3489 1210 1059 BsrBI CCG'CTC Cuts at: 1394 1823 3645 1394 Size: 429 1822 3507 Fragments arranged by size: 3507 1822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2399 4253 4427 65 Size: 1183 1151 1854 174 1396 Fragments arranged by size: 1854 1396 1183 1151 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4402 4589 1700 Size: 110 2592 187 2869 Fragments arranged by size: 2869 2592 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5758 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstXI CCAn_nnnn'nTGG Cuts at: 2840 2840 Size: 5758 BstYI r'GATC_y Cuts at: 1972 2263 2650 3088 3455 3840 3857 5309 5395 Size: 291 387 438 367 385 17 1452 86 Cuts at: 5395 5407 5753 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 438 387 385 367 346 291 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2343 2848 513 Size: 552 939 339 505 3423 Fragments arranged by size: 3423 939 552 505 339 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5019 Fragments arranged by size: 5019 683 56 BtsI GCAGTG_nn' Cuts at: 2514 2697 2940 3274 4068 4088 2514 Size: 183 243 334 794 20 4184 Fragments arranged by size: 4184 794 334 243 183 20 ClaI AT'CG_AT Cuts at: 1554 2337 1554 Size: 783 4975 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2183 2218 529 Size: 35 1619 35 4069 Fragments arranged by size: 4069 1619 35 35 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DraIII CAC_nnn'GTG Cuts at: 2819 2819 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3023 3205 4094 Size: 54 1425 50 275 18 1193 182 889 Cuts at: 4094 5723 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1193 889 275 182 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3205 1537 Size: 275 1393 4090 Fragments arranged by size: 4090 1393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2240 2582 3688 1353 Size: 887 342 1106 3423 Fragments arranged by size: 3423 1106 887 342 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4345 4719 4865 387 Size: 1064 138 2756 374 146 1280 Fragments arranged by size: 2756 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 2762 2764 2997 3821 4418 5205 Size: 393 632 498 2 233 824 597 787 Cuts at: 5205 5473 1239 Size: 268 1524 Fragments arranged by size: 1524 824 787 632 597 498 393 268 233 2 EcoO109I rG'GnC_Cy Cuts at: 2722 2723 2800 2722 Size: 1 77 5680 Fragments arranged by size: 5680 77 1 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2167 2167 Size: 5758 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 4905 1678 Size: 3227 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2183 2215 4399 4431 4473 Size: 32 135 32 611 32 2184 32 42 Cuts at: 4473 4505 1373 Size: 32 2626 Fragments arranged by size: 2626 2184 611 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3102 3348 669 Size: 988 281 1164 246 3079 Fragments arranged by size: 3079 1164 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2286 4872 5056 1305 Size: 981 2586 184 2007 Fragments arranged by size: 2586 2007 981 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3025 10 Size: 54 1425 343 1193 2743 Fragments arranged by size: 2743 1425 1193 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2091 3716 4075 4234 518 Size: 648 925 1625 359 159 2042 Fragments arranged by size: 2042 1625 925 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2784 3174 3851 4999 5244 1606 Size: 435 743 390 677 1148 245 2120 Fragments arranged by size: 2120 1148 743 677 435 390 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2848 513 Size: 552 939 844 3423 Fragments arranged by size: 3423 939 844 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 2767 3116 4661 1132 Size: 97 1538 349 1545 2229 Fragments arranged by size: 2229 1545 1538 349 97 PasI CC'CwG_GG Cuts at: 1348 2734 1348 Size: 1386 4372 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2012 2044 3827 3859 5340 5372 2012 Size: 32 1783 32 1481 32 2398 Fragments arranged by size: 2398 1783 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PspOMI G'GGCC_C Cuts at: 2722 2722 Size: 5758 PstI C_TGCA'G Cuts at: 830 2692 2965 3110 830 Size: 1862 273 145 3478 Fragments arranged by size: 3478 1862 273 145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SfcI C'TryA_G Cuts at: 826 1045 2688 2961 3106 4241 4922 5113 826 Size: 219 1643 273 145 1135 681 191 1471 Fragments arranged by size: 1643 1471 1135 681 273 219 191 145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2453 3862 4763 5025 820 Size: 197 926 510 1409 901 262 1553 Fragments arranged by size: 1553 1409 926 901 510 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 2767 3116 1132 Size: 1635 349 3774 Fragments arranged by size: 3774 1635 349 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5758 StyI C'CwwG_G Cuts at: 513 1065 2004 2848 3197 513 Size: 552 939 844 349 3074 Fragments arranged by size: 3074 939 844 552 349 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2423 3785 3802 3955 4140 4585 325 Size: 1255 843 1362 17 153 185 445 1498 Fragments arranged by size: 1498 1362 1255 843 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2273 4004 5716 Size: 274 80 33 51 1443 296 1731 1712 Cuts at: 5716 96 Size: 138 Fragments arranged by size: 1731 1712 1443 296 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2829 2861 5718 5750 2829 Size: 32 2857 32 2837 Fragments arranged by size: 2857 2837 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI