Restriction map for
pFN21AE0172
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# NO CUT ENZYMES ( 139 enzymes) #
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Acc65I AciI AclI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaI BsiEI BsiYI BslI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HindIII HpaI KasI KpnI
KspI MfeI MluI MspA1I MunI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 77 enzymes) #
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AatII 837 GACGTC GACGT^C
AccI 595 GTAGAC GT^[AC][GT]AC
AccII 250 CGCG CG^CG
AccIII 443 TCCGGA T^CCGGA
AcsI 133 GAATTT [GA]^AATT[CT]
AcyI 837 GACGTC G[GA]^CG[CT]C
AfaI 126 GTAC GT^AC
AflII 435 CTTAAG C^TTAAG
AlwNI 509 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 120 GGGCCC GGGCC^C
ApoI 133 GAATTT [GA]^AATT[CT]
Asp700I 108 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 225 GTGCTC G[AT]GC[AT]^C
AspI 598 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 351 GGACC G^G[AT]CC
BalI 71 TGGCCA TGG^CCA
BanI 810 GGCACC G^G[CT][GA]CC
BbsI 298 GTCTTC GAAGAC(2/6)
BbvCI 415 CCTCAGC CCTCAGC(-5/-2)
BbvI 5 GCTGC GCAGC(8/12)
BfaI 890 CTAG C^TAG
BfrI 435 CTTAAG C^TTAAG
BpmI 475 CTGGAG CTGGAG(16/14)
BpuAI 298 GTCTTC GAAGAC(2/6)
BsaBI 555 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 837 GACGTC G[GA]^CG[CT]C
BsaWI 443 TCCGGA [AT]^CCGG[AT]
BseAI 443 TCCGGA T^CCGGA
BsiHKAI 225 GTGCTC G[AT]GC[AT]^C
BsiWI 125 CGTACG C^GTACG
BsmBI 839 CGTCTC CGTCTC(1/5)
BsmI 826 GCATTC GAATGC(1/-1)
BspEI 443 TCCGGA T^CCGGA
BsrI 218 ACTGG ACTGG(1/-1)
BssSI 385 CACGAG CACGAG(-5/-1)
BstF5I 66 GGATG GGATG(2/0)
BstUI 250 CGCG CG^CG
BstXI 714 CCATGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 885 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 249 GCGC GCG^C
DraII 272 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 62 CCGTGG C^C[GA][CT]GG
EaeI 71 TGGCCA [CT]^GGCC[GA]
EcoO109I 272 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 361 GATATC GAT^ATC
EcoT14I 269 CCAAGG C^C[AT][AT]GG
Fnu4HI 5 GCTGC GC^[ACGT]GC
FokI 66 GGATG GGATG(9/13)
HapII 444 CCGG C^CGG
HhaI 249 GCGC GCG^C
Hin1I 837 GACGTC G[GA]^CG[CT]C
HinP1I 249 GCGC G^CGC
HincII 861 GTCAAC GT[CT]^[GA]AC
HindII 861 GTCAAC GT[CT]^[GA]AC
HpaII 444 CCGG C^CGG
HphI 97 TCACC GGTGA(8/7)
ItaI 5 GCTGC GC^[ACGT]GC
MaeI 890 CTAG C^TAG
MaeIII 505 GTGAC ^GT[ACGT]AC
MamI 555 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 71 TGGCCA TGG^CCA
MroI 443 TCCGGA T^CCGGA
MscI 71 TGGCCA TGG^CCA
MspI 444 CCGG C^CGG
MvnI 250 CGCG CG^CG
PflFI 598 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 334 CTGCAG CTGCA^G
RsaI 126 GTAC GT^AC
SapI 897 GCTCTTC GCTCTTC(1/4)
SfcI 334 CTGCAG C^T[GA][CT]AG
SmlI 435 CTTAAG C^T[CT][GA]AG
StyI 269 CCAAGG C^C[AT][AT]GG
TfiI 571 GATTC G^A[AT]TC
TseI 5 GCTGC G^C[AT]GC
Tsp45I 505 GTGAC ^GT[GC]AC
Tth111I 598 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 108 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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AluI 4 AGCT AG^CT
916 AGCT
BanII 120 GGGCCC G[GA]GC[CT]^C
895 GGGCTC
BseRI 366 CTCCTC GAGGAG(10/8)
388 GAGGAG
BsgI 6 CTGCAC GTGCAG(16/14)
796 GTGCAG
BsmAI 182 GTCTC GTCTC(1/5)
840 GTCTC
BsmFI 511 GGGAC GGGAC(10/14)
737 GGGAC
EarI 898 CTCTTC CTCTTC(1/4)
108 GAAGAG
Ksp632I 898 CTCTTC CTCTTC(1/4)
108 GAAGAG
PleI 265 GACTC GAGTC(4/5)
859 GAGTC
TaqI 569 TCGA T^CGA
948 TCGA
TthHB8I 569 TCGA T^CGA
948 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AlwI BmyI BsaJI Bsp1286I BssKI
BstNI BstYI Cfr13I DdeI DpnII
EcoRII HaeIII HinfI MaeII MboI
MboII MflI MnlI MseI MslI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI Tru9I Tsp509I TspRI
XhoII