Restriction map for
pFN21AE0172
(Linear) (Six-base) MAPSORT of: KIEE0172.seq Check: 9062 from: 1 to: 951
KIEE0172.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AatII G_ACGT'C
Cuts at: 0 841 951
Size: 841 110
AccI GT'mk_AC
Cuts at: 0 596 951
Size: 596 355
AflII C'TTAA_G
Cuts at: 0 435 951
Size: 435 516
AlwNI CAG_nnn'CTG
Cuts at: 0 514 951
Size: 514 437
ApaI G_GGCC'C
Cuts at: 0 124 951
Size: 124 827
ApoI r'AATT_y
Cuts at: 0 133 951
Size: 133 818
BanI G'GyrC_C
Cuts at: 0 810 951
Size: 810 141
BanII G_rGCy'C
Cuts at: 0 124 899 951
Size: 124 775 52
Fragments arranged by size:
775 124 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 291 951
Size: 291 660
BbvCI CC'TCA_GC
Cuts at: 0 416 951
Size: 416 535
Bme1580I G_kGCm'C
Cuts at: 0 124 951
Size: 124 827
BmgBI CAC'GTC
Cuts at: 0 37 951
Size: 37 914
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 501 951
Size: 501 450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 951
Size: 496 455
Bpu10I CC'TnA_GC
Cuts at: 0 416 951
Size: 416 535
BsaBI GATnn'nnATC
Cuts at: 0 559 951
Size: 559 392
BsaHI Gr'CG_yC
Cuts at: 0 838 951
Size: 838 113
BsaWI w'CCGG_w
Cuts at: 0 443 951
Size: 443 508
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 357 403 951
Size: 357 46 548
Fragments arranged by size:
548 357 46
BseYI C'CCAG_C
Cuts at: 0 917 951
Size: 917 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 951
Size: 817 134
BsiHKAI G_wGCw'C
Cuts at: 0 229 951
Size: 229 722
BsiWI C'GTAC_G
Cuts at: 0 125 951
Size: 125 826
BsmI GAATG_Cn'
Cuts at: 0 826 951
Size: 826 125
BsmBI CGTCTCn'nnnn_
Cuts at: 0 845 951
Size: 845 106
Bsp1286I G_dGCh'C
Cuts at: 0 124 229 899 951
Size: 124 105 670 52
Fragments arranged by size:
670 124 105 52
BspEI T'CCGG_A
Cuts at: 0 443 951
Size: 443 508
BssSI C'ACGA_G
Cuts at: 0 385 951
Size: 385 566
BstXI CCAn_nnnn'nTGG
Cuts at: 0 721 951
Size: 721 230
BstYI r'GATC_y
Cuts at: 0 30 330 554 951
Size: 30 300 224 397
Fragments arranged by size:
397 300 224 30
BtgI C'CryG_G
Cuts at: 0 62 951
Size: 62 889
BtsI GCAGTG_nn'
Cuts at: 0 284 951
Size: 284 667
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 23 58 951
Size: 23 35 893
Fragments arranged by size:
893 35 23
EaeI y'GGCC_r
Cuts at: 0 71 951
Size: 71 880
EarI CTCTTCn'nnn_
Cuts at: 0 103 904 951
Size: 103 801 47
Fragments arranged by size:
801 103 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 951
Size: 496 455
EcoO109I rG'GnC_Cy
Cuts at: 0 273 951
Size: 273 678
EcoRV GAT'ATC
Cuts at: 0 363 951
Size: 363 588
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 631 663 844 876 951
Size: 352 32 247 32 181 32 75
Fragments arranged by size:
352 247 181 75 32 32 32
HincII GTy'rAC
Cuts at: 0 863 951
Size: 863 88
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 703 951
Size: 329 374 248
Fragments arranged by size:
374 329 248
MscI TGG'CCA
Cuts at: 0 73 951
Size: 73 878
MslI CAynn'nnrTG
Cuts at: 0 13 61 719 951
Size: 13 48 658 232
Fragments arranged by size:
658 232 48 13
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 925 951
Size: 925 26
PspOMI G'GGCC_C
Cuts at: 0 120 951
Size: 120 831
PstI C_TGCA'G
Cuts at: 0 338 951
Size: 338 613
SapI GCTCTTCn'nnn_
Cuts at: 0 904 951
Size: 904 47
SfcI C'TryA_G
Cuts at: 0 334 951
Size: 334 617
SmlI C'TyrA_G
Cuts at: 0 435 951
Size: 435 516
StyI C'CwwG_G
Cuts at: 0 269 951
Size: 269 682
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 42 951
Size: 42 909
Tth111I GACn'n_nGTC
Cuts at: 0 601 951
Size: 601 350
XmnI GAAnn'nnTTC
Cuts at: 0 112 951
Size: 112 839
ZraI GAC'GTC
Cuts at: 0 839 951
Size: 839 112
Enzymes that do cut:
AatII AccI AflII AlwNI ApaI ApoI BanI BanII
BbsI BbvCI Bme1580I BmgBI BplI BpmI Bpu10I BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BssSI BstXI BstYI BtgI BtsI
CspCI EaeI EarI Eco57MI EcoO109I EcoRV Hin4I HincII
MmeI MscI MslI PpiI PspOMI PstI SapI SfcI
SmlI StyI TaqII Tth111I XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI BpuEI BsaI BsaAI
BsaXI BsiEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI XbaI XcmI XhoI XmaI