(Circular) (Six-base) MAPSORT of: pFN21AB7064.seq Check: 4800 from: 1 to: 6727 pFN21AB7064 6727 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4537 278 Size: 53 83 186 936 3001 2468 Fragments arranged by size: 3001 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4070 1937 Size: 2133 4594 Acc65I G'GTAC_C Cuts at: 4048 4048 Size: 6727 AclI AA'CG_TT Cuts at: 1563 4854 5227 1563 Size: 3291 373 3063 Fragments arranged by size: 3291 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2262 2274 2340 2532 2745 3041 3424 4790 Size: 630 12 66 192 213 296 383 1366 Cuts at: 4790 6174 6442 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1366 630 383 296 268 213 192 66 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6530 AflIII A'CryG_T Cuts at: 1203 5626 6603 1203 Size: 4423 977 1327 Fragments arranged by size: 4423 1327 977 AgeI A'CCGG_T Cuts at: 5558 5558 Size: 6727 AhdI GACnn_n'nnGTC Cuts at: 2776 5456 2776 Size: 2680 4047 AleI CACnn'nnGTG Cuts at: 1166 2663 1166 Size: 1497 5230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6727 AlwNI CAG_nnn'CTG Cuts at: 2781 3447 3849 3998 4470 6042 2781 Size: 666 402 149 472 1572 3466 Fragments arranged by size: 3466 1572 666 472 402 149 ApaI G_GGCC'C Cuts at: 3436 3746 3436 Size: 310 6417 ApaLI G'TGCA_C Cuts at: 4784 5940 4784 Size: 1156 5571 ApoI r'AATT_y Cuts at: 1493 4036 4262 4414 6523 1493 Size: 2543 226 152 2109 1697 Fragments arranged by size: 2543 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5281 160 Size: 872 4249 1606 Fragments arranged by size: 4249 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6727 AvaI C'yCGr_G Cuts at: 1870 1943 3762 3837 4052 1870 Size: 73 1819 75 215 4545 Fragments arranged by size: 4545 1819 215 75 73 AvrII C'CTAG_G Cuts at: 3578 3578 Size: 6727 BamHI G'GATC_C Cuts at: 2645 4057 4424 2645 Size: 1412 367 4948 Fragments arranged by size: 4948 1412 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2913 4048 5503 618 Size: 325 217 627 1126 1135 1455 1842 Fragments arranged by size: 1842 1455 1135 1126 627 325 217 BanII G_rGCy'C Cuts at: 1391 2136 3436 3516 3574 3746 3818 4046 1391 Size: 745 1300 80 58 172 72 228 4072 Fragments arranged by size: 4072 1300 745 228 172 80 72 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2262 2744 3148 928 Size: 971 363 482 404 4507 Fragments arranged by size: 4507 971 482 404 363 BbvCI CC'TCA_GC Cuts at: 2090 2875 3123 3492 2090 Size: 785 248 369 5325 Fragments arranged by size: 5325 785 369 248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3743 3760 3777 3794 4918 4952 1903 Size: 34 1806 17 17 17 1124 34 3678 Fragments arranged by size: 3678 1806 1124 34 34 17 17 17 BciVI GTATCCnnnnn_n' Cuts at: 4619 5829 4619 Size: 1210 5517 BclI T'GATC_A Cuts at: 1548 1548 Size: 6727 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2115 5338 136 Size: 107 122 71 631 605 443 3223 1525 Fragments arranged by size: 3223 1525 631 605 443 122 107 71 BglII A'GATC_T Cuts at: 6722 6722 Size: 6727 BlpI GC'TnA_GC Cuts at: 3510 3812 4144 3510 Size: 302 332 6093 Fragments arranged by size: 6093 332 302 Bme1580I G_kGCm'C Cuts at: 1790 3436 3746 3856 4788 5944 1790 Size: 1646 310 110 932 1156 2573 Fragments arranged by size: 2573 1646 1156 932 310 110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6727 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2069 2300 2850 Size: 416 53 83 399 24 652 231 550 Cuts at: 2850 2916 3508 3926 4458 5416 442 Size: 66 592 418 532 958 1753 Fragments arranged by size: 1753 958 652 592 550 532 418 416 399 231 83 66 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6727 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3148 3148 Size: 6727 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2122 2204 2292 2625 3567 5387 1239 Size: 883 82 88 333 942 1820 2579 Fragments arranged by size: 2579 1820 942 883 333 88 82 Bpu10I CC'TnA_GC Cuts at: 2090 2875 3123 3492 2090 Size: 785 248 369 5325 Fragments arranged by size: 5325 785 369 248 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2885 3593 3970 4852 5717 6015 2885 Size: 708 377 882 865 298 3597 Fragments arranged by size: 3597 882 865 708 377 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5390 882 Size: 617 3891 2219 Fragments arranged by size: 3891 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4518 493 Size: 711 3314 2702 Fragments arranged by size: 3314 2702 711 BsaBI GATnn'nnATC Cuts at: 6721 6721 Size: 6727 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2236 2804 4534 4916 Size: 53 83 186 936 703 568 1730 382 Cuts at: 4916 5551 275 Size: 635 1451 Fragments arranged by size: 1730 1451 936 703 635 568 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3637 5160 5558 5832 5979 6390 3637 Size: 1523 398 274 147 411 3974 Fragments arranged by size: 3974 1523 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6502 Fragments arranged by size: 6502 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2193 2261 2498 2531 2591 2594 2825 Size: 892 115 68 237 33 60 3 231 Cuts at: 2825 2948 3212 3215 3341 3344 3564 3821 3904 Size: 123 264 3 126 3 220 257 83 Cuts at: 3904 1186 Size: 4009 Fragments arranged by size: 4009 892 264 257 237 231 220 126 123 115 83 68 60 33 3 3 3 BseYI C'CCAG_C Cuts at: 2435 2654 2881 2895 3447 5930 6426 2435 Size: 219 227 14 552 2483 496 2736 Fragments arranged by size: 2736 2483 552 496 227 219 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6154 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4177 4938 5966 1540 Size: 275 186 2176 761 1028 2301 Fragments arranged by size: 2301 2176 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2425 4788 4873 5944 2425 Size: 2363 85 1071 3208 Fragments arranged by size: 3208 2363 1071 85 BsmI GAATG_Cn' Cuts at: 4238 4331 4238 Size: 93 6634 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2425 3436 3516 3574 3746 3818 Size: 399 346 289 1011 80 58 172 72 Cuts at: 3818 3856 4046 4788 4873 5944 1391 Size: 38 190 742 85 1071 2174 Fragments arranged by size: 2174 1071 1011 742 399 346 289 190 172 85 80 72 58 38 BspEI T'CCGG_A Cuts at: 3637 6390 3637 Size: 2753 3974 BspHI T'CATG_A Cuts at: 3631 4616 6346 3631 Size: 985 1730 4012 Fragments arranged by size: 4012 1730 985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4082 844 Size: 3238 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3978 4614 1394 Size: 429 2155 636 3507 Fragments arranged by size: 3507 2155 636 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5222 5396 65 Size: 1183 3974 174 1396 Fragments arranged by size: 3974 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3721 5371 5558 1700 Size: 110 1911 1650 187 2869 Fragments arranged by size: 2869 1911 1650 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6727 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6727 BssSI C'ACGA_G Cuts at: 4787 5799 4787 Size: 1012 5715 BstAPI GCAn_nnn'nTGC Cuts at: 794 3998 794 Size: 3204 3523 BstBI TT'CG_AA Cuts at: 5531 5531 Size: 6727 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6727 BstYI r'GATC_y Cuts at: 1972 2645 4057 4424 4809 4826 6278 6364 6376 Size: 673 1412 367 385 17 1452 86 12 Cuts at: 6376 6722 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1412 673 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2111 3860 2111 Size: 1749 4978 BtgI C'CryG_G Cuts at: 513 1065 2004 2039 2571 2697 513 Size: 552 939 35 532 126 4543 Fragments arranged by size: 4543 939 552 532 126 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5988 Fragments arranged by size: 5988 683 56 BtsI GCAGTG_nn' Cuts at: 2453 4243 5037 5057 2453 Size: 1790 794 20 4123 Fragments arranged by size: 4123 1790 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6727 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6692 DraI TTT'AAA Cuts at: 4031 4031 Size: 6727 DraIII CAC_nnn'GTG Cuts at: 3011 3011 Size: 6727 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2746 5734 809 Size: 507 1430 2988 1802 Fragments arranged by size: 2988 1802 1430 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2413 3038 3308 Size: 54 1425 50 275 18 583 625 270 Cuts at: 3308 3531 3963 4174 5063 6692 8 Size: 223 432 211 889 1629 43 Fragments arranged by size: 1629 1425 889 625 583 432 275 270 223 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4174 1537 Size: 275 2362 4090 Fragments arranged by size: 4090 2362 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2238 2493 2508 2679 2925 3054 3105 3139 Size: 885 255 15 171 246 129 51 34 Cuts at: 3139 3474 4657 1353 Size: 335 1183 3423 Fragments arranged by size: 3423 1183 885 335 255 246 171 129 51 34 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5314 5688 5834 387 Size: 1064 138 3725 374 146 1280 Fragments arranged by size: 3725 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 2204 2262 2274 2292 2340 2532 Size: 393 490 82 58 12 18 48 192 Cuts at: 2532 2625 2745 3041 3424 3567 4790 5387 6174 Size: 93 120 296 383 143 1223 597 787 Cuts at: 6174 6442 1239 Size: 268 1524 Fragments arranged by size: 1524 1223 787 597 490 393 383 296 268 192 143 120 93 82 58 48 18 12 EcoNI CCTnn'n_nnAGG Cuts at: 3147 3147 Size: 6727 EcoO109I rG'GnC_Cy Cuts at: 2116 2123 2267 2778 2892 3432 3742 3743 2116 Size: 7 144 511 114 540 310 1 5100 Fragments arranged by size: 5100 540 511 310 144 114 7 1 EcoRI G'AATT_C Cuts at: 4036 4414 4036 Size: 378 6349 EcoRV GAT'ATC Cuts at: 3455 3455 Size: 6727 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3691 3691 Size: 6727 FseI GG_CCGG'CC Cuts at: 3725 3725 Size: 6727 FspI TGC'GCA Cuts at: 5233 5233 Size: 6727 HaeII r_GCGC'y Cuts at: 1678 3616 5874 1678 Size: 1938 2258 2531 Fragments arranged by size: 2531 2258 1938 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2762 2794 3077 3083 3109 Size: 32 135 32 1190 32 283 6 26 Cuts at: 3109 3115 3212 3244 3519 3551 5368 5400 5442 Size: 6 97 32 275 32 1817 32 42 Cuts at: 5442 5474 1373 Size: 32 2626 Fragments arranged by size: 2626 1817 1190 283 275 135 97 42 32 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 2527 4071 4317 669 Size: 988 281 589 1544 246 3079 Fragments arranged by size: 3079 1544 988 589 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6727 HpaI GTT'AAC Cuts at: 2527 4317 2527 Size: 1790 4937 KpnI G_GTAC'C Cuts at: 4052 4052 Size: 6727 MfeI C'AATT_G Cuts at: 4326 4326 Size: 6727 MluI A'CGCG_T Cuts at: 6603 6603 Size: 6727 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2793 3616 5841 6025 1305 Size: 1488 823 2225 184 2007 Fragments arranged by size: 2225 2007 1488 823 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2415 3040 3533 3965 10 Size: 54 1425 343 583 625 493 432 2772 Fragments arranged by size: 2772 1425 625 583 493 432 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2663 4685 5044 5203 518 Size: 648 1497 2022 359 159 2042 Fragments arranged by size: 2042 2022 1497 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2963 3995 4143 4820 5968 6213 1606 Size: 1357 1032 148 677 1148 245 2120 Fragments arranged by size: 2120 1357 1148 1032 677 245 148 NaeI GCC'GGC Cuts at: 3723 3723 Size: 6727 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5236 Fragments arranged by size: 5236 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6727 NgoMIV G'CCGG_C Cuts at: 3721 3721 Size: 6727 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6727 NotI GC'GGCC_GC Cuts at: 4174 4174 Size: 6727 NspI r_CATG'y Cuts at: 1132 1229 2356 4085 5630 1132 Size: 97 1127 1729 1545 2229 Fragments arranged by size: 2229 1729 1545 1127 97 PasI CC'CwG_GG Cuts at: 1348 2477 2675 2861 3567 3747 1348 Size: 1129 198 186 706 180 4328 Fragments arranged by size: 4328 1129 706 198 186 180 PciI A'CATG_T Cuts at: 5626 5626 Size: 6727 PflMI CCAn_nnn'nTGG Cuts at: 1108 2661 2781 3703 3849 3961 1108 Size: 1553 120 922 146 112 3874 Fragments arranged by size: 3874 1553 922 146 120 112 PfoI T'CCnGG_A Cuts at: 2641 2641 Size: 6727 PmeI GTTT'AAAC Cuts at: 4031 4031 Size: 6727 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4796 4828 6309 6341 4796 Size: 32 1481 32 5182 Fragments arranged by size: 5182 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2123 2778 2123 Size: 655 6072 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6727 PsiI TTA'TAA Cuts at: 4297 4297 Size: 6727 PspOMI G'GGCC_C Cuts at: 3432 3742 3432 Size: 310 6417 PstI C_TGCA'G Cuts at: 830 3994 4079 830 Size: 3164 85 3478 Fragments arranged by size: 3478 3164 85 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6727 PvuII CAG'CTG Cuts at: 2963 2963 Size: 6727 SalI G'TCGA_C Cuts at: 1936 4069 1936 Size: 2133 4594 SbfI CC_TGCA'GG Cuts at: 4079 4079 Size: 6727 ScaI AGT'ACT Cuts at: 4975 6687 4975 Size: 1712 5015 SfcI C'TryA_G Cuts at: 826 1045 3147 3990 4075 5210 5891 6082 826 Size: 219 2102 843 85 1135 681 191 1471 Fragments arranged by size: 2102 1471 1135 843 681 219 191 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6727 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6727 SmaI CCC'GGG Cuts at: 1872 3839 4054 1872 Size: 1967 215 4545 Fragments arranged by size: 4545 1967 215 SmlI C'TyrA_G Cuts at: 820 1017 1943 2900 3608 3949 4831 5732 5994 Size: 197 926 957 708 341 882 901 262 Cuts at: 5994 820 Size: 1553 Fragments arranged by size: 1553 957 926 901 882 708 341 262 197 SnaBI TAC'GTA Cuts at: 493 4518 493 Size: 4025 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6727 SphI G_CATG'C Cuts at: 1132 4085 1132 Size: 2953 3774 SrfI GCCC'GGGC Cuts at: 3839 3839 Size: 6727 SspI AAT'ATT Cuts at: 5 52 4651 5 Size: 47 4599 2081 Fragments arranged by size: 4599 2081 47 StuI AGG'CCT Cuts at: 1517 3026 3296 1517 Size: 1509 270 4948 Fragments arranged by size: 4948 1509 270 StyI C'CwwG_G Cuts at: 513 1065 2004 2225 3384 3578 4166 513 Size: 552 939 221 1159 194 588 3074 Fragments arranged by size: 3074 1159 939 588 552 221 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2653 3019 3289 3809 3911 4754 4771 Size: 1255 1073 366 270 520 102 843 17 Cuts at: 4771 4924 5109 5554 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1073 843 520 445 366 270 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4973 6685 96 Size: 274 80 33 51 1443 2996 1712 138 Fragments arranged by size: 2996 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6687 6719 6687 Size: 32 6695 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6727 XbaI T'CTAG_A Cuts at: 2128 4063 2128 Size: 1935 4792 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3564 3564 Size: 6727 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6727 XmaI C'CCGG_G Cuts at: 1870 3837 4052 1870 Size: 1967 215 4545 Fragments arranged by size: 4545 1967 215 XmnI GAAnn'nnTTC Cuts at: 4856 4856 Size: 6727 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4535 276 Size: 53 83 186 936 3001 2468 Fragments arranged by size: 3001 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI